Targeted destruction of murine macrophage cells with bioconjugated gold nanorods
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Gold nanorods manifest a readily tunable longitudinal plasmon resonance with light and consequently have potential for use in photothermal therapeutics. Recent work by others has shown how gold nanoshells and rods can be used to target cancer cells, which can then be destroyed using relatively high power laser radiation (∼1×105 to 1×1010 W/m2). Here we extend this concept to demonstrate how gold nanorods can be modified to bind to target macrophage cells, and show that high intensity laser radiation is not necessary, with even 5×102 W/m2 being sufficient, provided that a total fluence of ∼30 J/cm2 is delivered. We used the murine cell line RAW 264.7 and the monoclonal antibody CD11b, raised against murine macrophages, as our model system and a 5 mW solid state diode laser as our energy source. Exposure of the cells labeled with gold nanorods to a laser fluence of 30 J/cm2 resulted in 81% cell death compared to only 0.9% in the control, non-labeled cells.
Keywordshyperthermal therapy plasmon resonance plasmonic heating active and passive targeting biomedicine nanobiotechnology
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The authors thank R. Wuhrer, K. McBean and G.␣Stockton of the University of Technology Sydney for technical assistance received.
- 1.Alves-Rosa F., C. Stanganelli, J. Cabrera, N. van Rooijen, M.S. Palermo, M.A. Isturiz, 2000. Treatment with liposome-encapsulated clodronate as a new strategic approach in the management of immune thrombocytopenic purpura in a mouse model. Blood. 96, 2834–2840Google Scholar
- 2.Behnke O., T. Ammitzboll, H. Jessen, M. Klokker, K. Nilausen, J. Tranum-Jensen, L. Olsson, 1986. Non-specific binding of protein-stabilized gold sols as a source of error in immunocytochemistry. Eur J Cell Biol. 41, 326–338Google Scholar
- 6.Chang J.-Y., Wu, H., Chen, H., Lingb, Y.-C., Tan, W., 2005. Oriented assembly of Au nanorods using biorecognition system. Chem. Comm. 1092–1094Google Scholar
- 12.Draine B.T., P.J. Flatau, 1994. Discrete-dipole approximation for scattering calculations. J. Opt. Soc. Am. A. 11, 1491–1499Google Scholar
- 13.Draine, B. T., Flatau, P. J., 2004. User Guide for the Discrete Dipole Approximation Code DDSCAT 6.1, http://arxiv.org/abs/astro-ph/0309069, accessed January 2005Google Scholar
- 21.Hayat M.A., 1989. Colloidal Gold: Principles, Methods, and Applications. Academic Press, San Diego, CAGoogle Scholar
- 35.Link, S., El-Sayed, M. A., 2005. Simulation of the optical absorption spectra of gold nanorods as a function of their aspect ratio and the effect of the medium dielectric constant (103B correction). J. Phys. Chem. B 109, 20, 10531Google Scholar
- 36.Loo C., A. Lin, L. Hirsch, M.H. Lee, J. Barton, N. Halas, J. West, R. Drezek, 2004. Nanoshell-enabled photonics-based imaging and therapy of cancer. Technol. in Cancer Res., Treatment. 3, 33–40Google Scholar
- 46.Prasad V., A. Mikhailovsky, J.A. Zasadzinski, 2005. Langmuir 21, 7528–7532Google Scholar
- 48.Raub C.B., E.J. Orwin, R. Haskell, 2004. Immunogold labeling to enhance contrast in optical coherence microscopy of tissue engineered corneal constructs. 26th Annual Conference of the Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society, San Francisco, CAGoogle Scholar
- 49.Salata, O., 2004. Applications of nanoparticles in biology and medicine. J. Nanobiotechnology 2, Paper 3Google Scholar
- 52.Slot J.W., H.J. Geuze, 1985. A new method of preparing gold probes for multiple-labelling cytochemistry. Eur. J. Cell Biol. 38, 87–93Google Scholar
- 53.Takahashi, H., Niidome, Y., Yamada, S., 2005. Controlled release of plasmid DNA from gold nanorods induced by pulsed near-infrared light. Chem. Comm. 2247–2249Google Scholar