, Volume 183, Issue 5, pp 765–775 | Cite as

Molecular Genotyping of Candida parapsilosis Species Complex

  • Sourour Neji
  • Ines Hadrich
  • Amine Ilahi
  • Houaida Trabelsi
  • Hedi Chelly
  • Nadia Mahfoudh
  • Fatma Cheikhrouhou
  • Hayet Sellami
  • Fattouma Makni
  • Ali Ayadi
Original Article



The Candida parapsilosis complex species has emerged as an important cause of human disease. The molecular identification of C. parapsilosis isolates at the species level can be helpful for epidemiological studies and then for the establishment of appropriate therapies and prophylactic measures.


The present study was undertaken to analyze 13 short tandem repeat (STR) markers (7 minisatellites and 6 microsatellites) in a global set of 182 C. parapsilosis complex isolates from different origins including invasive and superficial clinical sites.


Upon the analysis of 182 strains of C. parapsilosis complex species, 10–17 haplotypes were detected for each minisatellite marker. The combination of 7 minisatellite markers yielded 121 different genotypes with a 0.995 D value. Upon the analysis of 114 isolates (68 from invasive infections and 46 from superficial infections), 21–32 genotypes were detected for each microsatellite marker. The combination of all 13 markers yielded 96 different genotypes among 114 isolates with a high degree of discrimination (0.997 D value).

The same multilocus genotype was shared by isolates recovered from some patients and from the hand of theirs correspondent healthcare worker. For another patient, the same multilocus genotype of C. metapsilosis was detected in blood and skin confirming that candidemia usually arises as an endogenous infection following prior colonization.


These STR markers are a valuable tool for the differentiation of C. parapsilosis complex strains, to support epidemiological investigations especially studies of strain relatedness and pathways of transmission.


Candida parapsilosis Candida orthopsilosis Candida metapsilosis STR Genetic diversity Genotyping 



We thank Dr Stephane Ranque for Candida parapsilosis reference isolates.

Compliance with ethical standards

Conflict of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.


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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Sourour Neji
    • 1
  • Ines Hadrich
    • 1
  • Amine Ilahi
    • 1
  • Houaida Trabelsi
    • 1
  • Hedi Chelly
    • 1
  • Nadia Mahfoudh
    • 2
  • Fatma Cheikhrouhou
    • 1
  • Hayet Sellami
    • 1
  • Fattouma Makni
    • 1
  • Ali Ayadi
    • 1
    • 3
  1. 1.Fungi and Parasitic Molecular Biology Laboratory, School of MedicineUniversity of SfaxSfaxTunisia
  2. 2.Laboratory of ImmunologyUH Hedi ChakerSfaxTunisia
  3. 3.Laboratoire de Biologie Moléculaire Parasitaire et FongiqueFaculté de Médecine de SfaxSfaxTunisia

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