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Molecular Genotyping of Candida parapsilosis Species Complex

  • Sourour Neji
  • Ines Hadrich
  • Amine Ilahi
  • Houaida Trabelsi
  • Hedi Chelly
  • Nadia Mahfoudh
  • Fatma Cheikhrouhou
  • Hayet Sellami
  • Fattouma Makni
  • Ali Ayadi
Original Article

Abstract

Background

The Candida parapsilosis complex species has emerged as an important cause of human disease. The molecular identification of C. parapsilosis isolates at the species level can be helpful for epidemiological studies and then for the establishment of appropriate therapies and prophylactic measures.

Methods

The present study was undertaken to analyze 13 short tandem repeat (STR) markers (7 minisatellites and 6 microsatellites) in a global set of 182 C. parapsilosis complex isolates from different origins including invasive and superficial clinical sites.

Results

Upon the analysis of 182 strains of C. parapsilosis complex species, 10–17 haplotypes were detected for each minisatellite marker. The combination of 7 minisatellite markers yielded 121 different genotypes with a 0.995 D value. Upon the analysis of 114 isolates (68 from invasive infections and 46 from superficial infections), 21–32 genotypes were detected for each microsatellite marker. The combination of all 13 markers yielded 96 different genotypes among 114 isolates with a high degree of discrimination (0.997 D value).

The same multilocus genotype was shared by isolates recovered from some patients and from the hand of theirs correspondent healthcare worker. For another patient, the same multilocus genotype of C. metapsilosis was detected in blood and skin confirming that candidemia usually arises as an endogenous infection following prior colonization.

Conclusions

These STR markers are a valuable tool for the differentiation of C. parapsilosis complex strains, to support epidemiological investigations especially studies of strain relatedness and pathways of transmission.

Keywords

Candida parapsilosis Candida orthopsilosis Candida metapsilosis STR Genetic diversity Genotyping 

Notes

Acknowledgements

We thank Dr Stephane Ranque for Candida parapsilosis reference isolates.

Compliance with ethical standards

Conflict of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

References

  1. 1.
    Trofa D, Gacser A, Nosanchuk JD. Candida parapsilosis, an emerging fungal pathogen. Clin Microbiol Rev. 2008;21(4):606–25.CrossRefPubMedPubMedCentralGoogle Scholar
  2. 2.
    van Asbeck EC, Clemons KV, Stevens DA. Candida parapsilosis: a review of its epidemiology, pathogenesis, clinical aspects, typing and antimicrobial susceptibility. Crit Rev Microbiol. 2009;35(4):283–309.CrossRefPubMedGoogle Scholar
  3. 3.
    Tavanti A, Davidson AD, Gow NA, Maiden MC, Odds FC. Candida orthopsilosis and Candida metapsilosis spp. nov. to replace Candida parapsilosis groups II and III. J Clin Microbiol. 2005;43(1):284–92.CrossRefPubMedPubMedCentralGoogle Scholar
  4. 4.
    Sabino R, Sampaio P, Rosado L, Stevens DA, Clemons KV, Pais C. New polymorphic microsatellite markers able to distinguish among Candida parapsilosis sensu stricto isolates. J Clin Microbiol. 2010;48(5):1677–82.CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    Garcia-Effron G, Canton E, Peman J, Dilger A, Roma E, Perlin DS. Assessment of two new molecular methods for identification of Candida parapsilosis sensu lato species. J Clin Microbiol. 2011;49(9):3257–61.CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Diab-Elschahawi M, Forstner C, Hagen F, Meis JF, Lassnig AM, Presterl E, Klaassen CH. Microsatellite genotyping clarified conspicuous accumulation of Candida parapsilosis at a cardiothoracic surgery intensive care unit. J Clin Microbiol. 2012;50(11):3422–6.CrossRefPubMedPubMedCentralGoogle Scholar
  7. 7.
    Reiss E, Lasker BA, Lott TJ, Bendel CM, Kaufman DA, Hazen KC, Wade KC, McGowan KL, Lockhart SR. Genotyping of Candida parapsilosis from three neonatal intensive care units (NICUs) using a panel of five multilocus microsatellite markers: broad genetic diversity and a cluster of related strains in one NICU. Infect Genet Evol. 2012;12(8):1654–60.CrossRefPubMedGoogle Scholar
  8. 8.
    Lasker BA, Butler G, Lott TJ. Molecular genotyping of Candida parapsilosis group I clinical isolates by analysis of polymorphic microsatellite markers. J Clin Microbiol. 2006;44(3):750–9.CrossRefPubMedPubMedCentralGoogle Scholar
  9. 9.
    Brillowska-Dabrowska A, Schon T, Pannanusorn S, Lonnbro N, Bernhoff L, Bonnedal J, Haggstrom J, Wistedt A, Fernandez V, Arendrup MC. A nosocomial outbreak of Candida parapsilosis in southern Sweden verified by genotyping. Scand J Infect Dis. 2009;41(2):135–42.CrossRefPubMedGoogle Scholar
  10. 10.
    White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocols Guide Methods Appl. 1990;18:315–22.Google Scholar
  11. 11.
    Rousset F. genepop’007: a complete re-implementation of the genepop software for Windows and Linux. Mol Ecol Resour. 2008;8(1):103–6.CrossRefPubMedGoogle Scholar
  12. 12.
    Hunter PR, Gaston MA. Numerical index of the discriminatory ability of typing systems: an application of Simpson’s index of diversity. J Clin Microbiol. 1988;26(11):2465–6.PubMedPubMedCentralGoogle Scholar
  13. 13.
    Maiden M, Bygraves J, Feil E, Morelli G, Russell J, Urwin R, Zhang Q, Zhou J, Zurth K, Caugant D, Feavers I, Achtman M, Spratt B. Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms. Proc Natl Acad Sci U S A. 1998;95(6):3140–5.CrossRefPubMedPubMedCentralGoogle Scholar
  14. 14.
    Polzin TDSV. On Steiner trees and minimum spanning trees in hypergraphs. Oper Res Lett. 2003;31:12–20.CrossRefGoogle Scholar
  15. 15.
    Vaz C, Sampaio P, Clemons KV, Huang YC, Stevens DA, Pais C. Microsatellite multilocus genotyping clarifies the relationship of Candida parapsilosis strains involved in a neonatal intensive care unit outbreak. Diagn Microbiol Infect Dis. 2011;71(2):159–62.CrossRefPubMedGoogle Scholar
  16. 16.
    Shin JH, Shin DH, Song JW, Kee SJ, Suh SP, Ryang DW. Electrophoretic karyotype analysis of sequential Candida parapsilosis isolates from patients with persistent or pecurrent fungemia. J Clin Microbiol. 2001;39(4):1258–63.CrossRefPubMedPubMedCentralGoogle Scholar
  17. 17.
    Enger L, Joly S, Pujol C, Simonson P, Pfaller M, Soll DR. Cloning and characterization of a complex DNA fingerprinting probe for Candida parapsilosis. J Clin Microbiol. 2001;39(2):658–69.CrossRefPubMedPubMedCentralGoogle Scholar
  18. 18.
    Zancope-Oliveira RM, James MJ, Derossi AP, Sampaio JL, Muniz MM, Li RK, Nascimento AS, Peralta JM, Reiss E. Strain characterization of Candida parapsilosis fungemia by molecular typing methods. Eur J Clin Microbiol Infect Dis. 2000;19(7):514–20.CrossRefPubMedGoogle Scholar
  19. 19.
    Van Asbeck EC, Clemons KV, Markham AN, Stevens DA. Candida parapsilosis global epidemiology G. Molecular epidemiology of the global and temporal diversity of Candida parapsilosis. Scand J Infect Dis. 2008;40(10):827–34.CrossRefPubMedGoogle Scholar
  20. 20.
    Borman AM, Linton CJ, Oliver D, Palmer MD, Szekely A, Odds FC, Johnson EM. Pyrosequencing analysis of 20 nucleotides of internal transcribed spacer 2 discriminates Candida parapsilosis, Candida metapsilosis, and Candida orthopsilosis. J Clin Microbiol. 2009;47(7):2307–10.CrossRefPubMedPubMedCentralGoogle Scholar
  21. 21.
    Romeo O, Delfino D, Cascio A, Lo Passo C, Amorini M, Romeo D, Pernice I. Microsatellite-based genotyping of Candida parapsilosis sensu stricto isolates reveals dominance and persistence of a particular epidemiological clone among neonatal intensive care unit patients. Infect Genet Evol. 2013;13:105–8.CrossRefPubMedGoogle Scholar
  22. 22.
    Sabino R, Sampaio P, Rosado L, Videira Z, Grenouillet F, Pais C. Analysis of clinical and environmental Candida parapsilosis isolates by microsatellite genotyping—a tool for hospital infection surveillance. Clin Microbiol Infect. 2015;21(10):954-e1–958.CrossRefGoogle Scholar
  23. 23.
    da Silva Ruiz L, Montelli AC, Sugizaki Mde F, Da Silva EG, De Batista GC, Moreira D, Paula CR. Outbreak of fungemia caused by Candida parapsilosis in a neonatal intensive care unit: molecular investigation through microsatellite analysis. Rev Iberoam Micol. 2013;30(2):112–5.CrossRefPubMedGoogle Scholar
  24. 24.
    Delfino D, Scordino F, Pernice I, Lo Passo C, Galbo R, David A, Barberi I, Criseo G, Cascio A, Romeo O. Potential association of specific Candida parapsilosis genotypes, bloodstream infections and colonization of health workers’ hands. Clin Microbiol Infect. 2014;20(11):O946–51.CrossRefPubMedGoogle Scholar
  25. 25.
    Chillemi V, Lo Passo C, van Diepeningen AD, Rharmitt S, Delfino D, Cascio A, Nnadi NE, Cilo BD, Sampaio P, Tietz HJ, Peman J, Criseo G, Romeo O, Scordino F. Multilocus microsatellite analysis of European and African Candida glabrata isolates. Eur J Clin Microbiol Infect Dis. 2016;35(6):885–92.CrossRefPubMedGoogle Scholar
  26. 26.
    Pulcrano G, Roscetto E, Iula VD, Panellis D, Rossano F, Catania MR. MALDI-TOF mass spectrometry and microsatellite markers to evaluate Candida parapsilosis transmission in neonatal intensive care units. Eur J Clin Microbiol Infect Dis. 2012;31(11):2919–28.CrossRefPubMedGoogle Scholar

Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Sourour Neji
    • 1
  • Ines Hadrich
    • 1
  • Amine Ilahi
    • 1
  • Houaida Trabelsi
    • 1
  • Hedi Chelly
    • 1
  • Nadia Mahfoudh
    • 2
  • Fatma Cheikhrouhou
    • 1
  • Hayet Sellami
    • 1
  • Fattouma Makni
    • 1
  • Ali Ayadi
    • 1
    • 3
  1. 1.Fungi and Parasitic Molecular Biology Laboratory, School of MedicineUniversity of SfaxSfaxTunisia
  2. 2.Laboratory of ImmunologyUH Hedi ChakerSfaxTunisia
  3. 3.Laboratoire de Biologie Moléculaire Parasitaire et FongiqueFaculté de Médecine de SfaxSfaxTunisia

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