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Mycopathologia

, Volume 181, Issue 7–8, pp 547–553 | Cite as

Epidemiological Aspects of Dermatophytosis in Khuzestan, southwestern Iran, an Update

  • Ali Rezaei-Matehkolaei
  • Abdollah Rafiei
  • Koichi Makimura
  • Yvonne Gräser
  • Maral Gharghani
  • Batool Sadeghi-Nejad
Article

Abstract

Dermatophytosis is among the most common superficial mycoses in Iran. The purpose of this report was to update the clinical and mycological features of human dermatophytosis in the Khuzestan, southwestern Iran. In the framework of a one-year survey, a total of 4120 skin, hair and nail samples obtained from the outpatients with symptoms suggestive of tinea were analyzed by using direct microscopy, culture and molecular identification methods. Strains isolated from cultures were subjected to amplification of the nuclear rDNA ITS regions in a PCR assay followed by an early established RFLP analysis. For confirmation of species identification, 100 isolates as representatives of all presumable species were subjected to ITS sequencing. Infection was confirmed in 1123 individuals (27.25 %) in the age range of 1–89 years by direct microscopy and/or culture including 603 males versus 520 females. Frequencies of infections were the highest and the lowest in age groups of 21–30 and 11–20 years, respectively. Tinea corporis was the most prevalent clinical manifestation followed by tinea cruris, tinea capitis, tinea manuum, tinea pedis, tinea unguium, tinea faciei and tinea barbae. Trichophyton interdigitale (58.7 %) was the most dominant isolate followed by Epidermophyton floccosum (35.4 %), Microsporum canis (3 %), T. rubrum (1.5 %), T. species of Arthroderma benhamiae (0.5 %), T. tonsurans (0.3 %) and T. violaceum (0.3 %). Other species included M. gypseum, M. fulvum and T. verrucosum (each one 0.1 %). Such a high occurrence of infection with T. interdigitale, which has not been reported from Iran, is due to the use of accurate molecular methods based on new species concept in dermatophytes. The prevalence of dermatophytoses caused by zoophilic species remarkably increased and Trichophyton species of A. benhamiae has emerged as a new agent of dermatophytosis in southwestern Iran, while infections due to anthropophilic species, except E. floccosum, took a decreasing trend.

Keywords

Tinea Dermatophyte Epidemiology ITS Khuzestan 

Notes

Acknowledgments

This work was financially supported by Grant Number 91116 from Vice-Chancellor for Research Affairs of Ahvaz Jundishapur University of Medical Sciences.

Compliance with Ethical Standards

Conflict of interest

None.

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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Ali Rezaei-Matehkolaei
    • 1
    • 2
  • Abdollah Rafiei
    • 1
    • 3
  • Koichi Makimura
    • 4
  • Yvonne Gräser
    • 5
  • Maral Gharghani
    • 2
  • Batool Sadeghi-Nejad
    • 6
  1. 1.Health Research Institute, Infectious and Tropical Diseases Research CenterAhvaz Jundishapur University of Medical SciencesAhvazIran
  2. 2.Department of Medical Mycology, School of MedicineAhvaz Jundishapur University of Medical SciencesAhvazIran
  3. 3.Department of Medical Parasitology, School of MedicineAhvaz Jundishapur University of Medical SciencesAhvazIran
  4. 4.Laboratory of Space and Environmental Medicine, Graduate School of MedicineTeikyo UniversityTokyoJapan
  5. 5.Consiliary Laboratory for Dermatophytes, Institute of MicrobiologyCharité-Universitätsmedizin BerlinBerlinGermany
  6. 6.Abadan School of Medical sciencesAbadanIran

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