, Volume 181, Issue 3–4, pp 235–240 | Cite as

In Vitro Activities of Five Antifungal Drugs Against Opportunistic Agents of Aspergillus Nigri Complex

  • Hamid Badali
  • Hamed Fakhim
  • Fereshteh Zarei
  • Mojtaba Nabili
  • Afsane Vaezi
  • Nafiseh Poorzad
  • Somayeh Dolatabadi
  • Hossein Mirhendi


Black aspergilli, particularly Aspergillus niger and A. tubingensis, are the most common etiological agents of otomycosis followed by onychomycosis, pulmonary aspergillosis and aspergilloma. However, so far there is no systematic study on their antifungal susceptibility profiles. A collection of 124 clinical and environmental species of black aspergilli consisted of A. niger, A. tubingensis, A. uvarum. A. acidus and A. sydowii were verified by DNA sequencing of the partial β-tubulin gene. MICs of amphotericin B, itraconazole, voriconazole, posaconazole, and MECs of caspofungin were performed based on CLSI M38-A2. Posaconazole and caspofungin had the lowest MIC range (0.016–0.125 µg/ml and 0.008–0.031 µg/ml, respectively), followed by amphotericin B (0.25–4 µg/ml), voriconazole (0.125–16 µg/ml) and itraconazole (0.25 to >16) in an increasing order. Some strains of A. niger showed high MIC value for itraconazole and voriconazole (>16 µg/ml), in contrast only environmental isolates of A. tubingensis had high itraconazole MICs (>16 µg/ml). These results confirm that posaconazole and caspofungin are potential drugs for treatment of aspergillosis due to opportunistic agents of Aspergillus Nigri complex. However, in vivo efficacy remains to be determined.


Aspergillus Nigri complex In vitro susceptibility Partial β-tubulin (BTU) 



This study was financially supported by a grant from the School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran (no. 1106) which we gratefully acknowledge. We are grateful to Iman Haghani for excellent technical assistance and help with antifungal susceptibility testing.

Compliance with Ethical Standards

Conflict of interest

The authors report no conflicts of interest.


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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Hamid Badali
    • 1
  • Hamed Fakhim
    • 2
  • Fereshteh Zarei
    • 3
  • Mojtaba Nabili
    • 2
  • Afsane Vaezi
    • 2
  • Nafiseh Poorzad
    • 2
  • Somayeh Dolatabadi
    • 4
    • 5
  • Hossein Mirhendi
    • 6
  1. 1.Department of Medical Mycology and Parasitology, School of Medicine/Invasive Fungi Research CenterMazandaran University of Medical SciencesSariIran
  2. 2.Student Research CommitteeMazandaran University of Medical SciencesSariIran
  3. 3.Department of Medical Mycology and Parasitology, School of Public HealthTehran University of Medical SciencesTehranIran
  4. 4.CBS-KNAW Fungal Biodiversity CentreUtrechtThe Netherlands
  5. 5.Cellular and Molecular Research CenterSabzevar University of Medical SciencesSabzevarIran
  6. 6.Department of Medical Parasitology and Mycology, School of MedicineIsfahan University of Medical SciencesIsfahanIran

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