In Vitro Activities of Five Antifungal Drugs Against Opportunistic Agents of Aspergillus Nigri Complex
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Black aspergilli, particularly Aspergillus niger and A. tubingensis, are the most common etiological agents of otomycosis followed by onychomycosis, pulmonary aspergillosis and aspergilloma. However, so far there is no systematic study on their antifungal susceptibility profiles. A collection of 124 clinical and environmental species of black aspergilli consisted of A. niger, A. tubingensis, A. uvarum. A. acidus and A. sydowii were verified by DNA sequencing of the partial β-tubulin gene. MICs of amphotericin B, itraconazole, voriconazole, posaconazole, and MECs of caspofungin were performed based on CLSI M38-A2. Posaconazole and caspofungin had the lowest MIC range (0.016–0.125 µg/ml and 0.008–0.031 µg/ml, respectively), followed by amphotericin B (0.25–4 µg/ml), voriconazole (0.125–16 µg/ml) and itraconazole (0.25 to >16) in an increasing order. Some strains of A. niger showed high MIC value for itraconazole and voriconazole (>16 µg/ml), in contrast only environmental isolates of A. tubingensis had high itraconazole MICs (>16 µg/ml). These results confirm that posaconazole and caspofungin are potential drugs for treatment of aspergillosis due to opportunistic agents of Aspergillus Nigri complex. However, in vivo efficacy remains to be determined.
KeywordsAspergillus Nigri complex In vitro susceptibility Partial β-tubulin (BTU)
This study was financially supported by a grant from the School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran (no. 1106) which we gratefully acknowledge. We are grateful to Iman Haghani for excellent technical assistance and help with antifungal susceptibility testing.
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Conflict of interest
The authors report no conflicts of interest.
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