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Mycopathologia

, Volume 181, Issue 1–2, pp 29–39 | Cite as

Combination of Estrogen and Immunosuppressive Agents to Establish a Mouse Model of Candidiasis with Concurrent Oral and Vaginal Mucosal Infection

  • Le Wang
  • Chong Wang
  • Huan Mei
  • Yongnian Shen
  • Guixia Lv
  • Rong Zeng
  • Ping Zhan
  • Dongmei LiEmail author
  • Weida LiuEmail author
Article

Abstract

Mouse model is an appropriate tool for pathogenic determination and study of host defenses during the fungal infection. Here, we established a mouse model of candidiasis with concurrent oral and vaginal mucosal infection. Two C. albicans strains sourced from clinical candidemia (SC5314) and mucosal infection (ATCC62342) were tested in ICR mice. The different combinational panels covering estrogen and immunosuppressive agents, cortisone, prednisolone and cyclophosphamide were used for concurrent oral and vaginal candidiasis establishment. Prednisolone in combination with estrogen proved an optimal mode for concurrent mucosal infection establishment. The model maintained for 1 week with fungal burden reached at least 105 cfu/g of tissue. This mouse model was evaluated by in vivo pharmacodynamics of fluconazole and host mucosal immunity of IL-17 and IL-23. Mice infected by SC5314 were cured by fluconazole. An increase in IL-23 in both oral and vaginal homogenates was observed after infection, while IL-17 only had a prominent elevation in oral tissue. This model could properly mimic complicated clinical conditions and provides a valuable means for antifungal assay in vivo and may also provide a useful method for the evaluation of host–fungal interactions.

Keywords

Candida albicans Mucosal candidiasis Mouse model Oral Vaginal 

Notes

Acknowledgments

We are grateful to Dr. Hu Bin for histopathological examination. This work was supported by National Key Basic Research Program of China (2013CB531605), National Natural Science Foundation of China (NO. 81071332) and Jiangsu Provincial Special Program of Medical Science (BL2012003).

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Le Wang
    • 1
  • Chong Wang
    • 1
  • Huan Mei
    • 1
  • Yongnian Shen
    • 1
  • Guixia Lv
    • 1
  • Rong Zeng
    • 1
  • Ping Zhan
    • 1
  • Dongmei Li
    • 2
    Email author
  • Weida Liu
    • 1
    Email author
  1. 1.Department of Mycology, Institute of Dermatology, Chinese Academy of Medical Science (CAMS)Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIsNanjingPeople’s Republic of China
  2. 2.Department of Microbiology and ImmunologyGeorgetown University Medical CenterWashingtonUSA

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