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Mycopathologia

, Volume 181, Issue 1–2, pp 107–113 | Cite as

Dermatophytosis due to Microsporum incurvatum: Notification and Identification of a Neglected Pathogenic Species

  • Ali Rezaei-Matehkolaei
  • Koichi Makimura
  • Yvonne Graser
  • Seyedmojtaba Seyedmousavi
  • Mahdi Abastabar
  • Abdollah Rafiei
  • Ping Zhan
  • Ali Ronagh
  • Sima Jafarpour
Article

Abstract

A 4-year-old Iranian boy developed erythematous, itchy and annular lesion on his face. Microscopic examination of the scraped samples with 10 % potassium hydroxide (KOH) revealed fungal septate hyphae and arthroconidia. The etiological agent was found to be Microsporum gypseum in mycological examinations. Amplification and restriction digestion of the internal transcribed spacers (ITS) of rDNA was not helpful for identification, but in ITS sequencing the isolate showed 98 % homology to Microsporum incurvatum strain CBS 172.64. Empirical treatment of the patient with griseofulvin for 4 weeks was successful. Other than our isolate, the ITS1 sequences of 38 strains from related species were retrieved from GenBank and phylogenetic tree using maximum likelihood method was constructed. The case isolate clustered apart from other strains of M. incurvatum. Pairwise comparison of ITS1 showed intraspecies variations of 0–13 nucleotides among M. incurvatum strains and an extensive interspecies variation of 33–80 bp and remarkable interspecies size polymorphism between the three sister species in the M. gypseum complex. The high level of ITS1 intraspecific variation is suitable for species identification rather than phylogeographic analysis of M. gypseum complex.

Keywords

Microsporum incurvatum Microsporum gypseum ITS Phylogeny 

Notes

Acknowledgments

This work was financially supported by grant number 91116 from Vice-Chancellor for Research Affairs of Ahvaz Jundishapur University of Medical Sciences.

Compliance with ethical standards

Conflict of interest

None.

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Ali Rezaei-Matehkolaei
    • 1
    • 2
  • Koichi Makimura
    • 3
    • 4
    • 5
  • Yvonne Graser
    • 6
  • Seyedmojtaba Seyedmousavi
    • 7
    • 8
    • 9
  • Mahdi Abastabar
    • 10
  • Abdollah Rafiei
    • 11
  • Ping Zhan
    • 12
  • Ali Ronagh
    • 13
  • Sima Jafarpour
    • 1
    • 2
  1. 1.Department of Medical Mycology, School of MedicineAhvaz Jundishapur University of Medical SciencesAhvazIslamic Republic of Iran
  2. 2.Health Research Institute, Infectious and Tropical Diseases Research CenterAhvaz Jundishapur University of Medical SciencesAhvazIslamic Republic of Iran
  3. 3.Laboratory of Space and Environmental Medicine, Graduate School of MedicineTeikyo UniversityTokyoJapan
  4. 4.Asia International Institute of Infectious Diseases ControlTeikyo UniversityTokyoJapan
  5. 5.Teikyo University Institute of Medical MycologyTokyoJapan
  6. 6.Consiliary Laboratory for Dermatophytes, Institute of MicrobiologyCharité-Universitätsmedizin BerlinBerlinGermany
  7. 7.Department of Medical MicrobiologyRadboud UMCNijmegenThe Netherlands
  8. 8.Department of Medical Microbiology and Infectious DiseasesErasmusMCRotterdamThe Netherlands
  9. 9.Invasive Fungi Research CenterMazandaran University of Medical SciencesSariIslamic Republic of Iran
  10. 10.Department of Medical Mycology and Parasitology, Invasive Fungi Research Center, School of MedicineMazandaran University of Medical SciencesSariIslamic Republic of Iran
  11. 11.Department of Medical Laboratory Sciences, Paramedical FacultyAhvaz Jundishapur University of Medical SciencesAhvazIslamic Republic of Iran
  12. 12.Dermatology Hospital of Jiangxi Province and Jiangxi Provincial Institute of DermatologyNanchangChina
  13. 13.Khuzestan Administration of Environmental ProtectionAhvazIslamic Republic of Iran

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