Sequence-Based Identification of a Zoophilic Strain of Trichophyton interdigitale in a Rare Case of Tinea Blepharo-Ciliaris Associated with Tinea Barbae
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Impaired hair at blepharo-ciliaris area by dermatophytes is a rare clinical entity. This infection is often misdiagnosed or underdiagnosed, being mistakenly referred to as an infection of bacterial origin. Herein, we present a rare case of tinea blepharo-ciliaris associated with tinea barbae in an adult male. Considering the two lesions of the patient, mycological examination was performed by phenotypic methods, including environmental electronic scanning microscopy. Trichophyton interdigitale zoophilic strain was identified as the etiological agent by direct examination of the hair, primary culture analysis of the developed colonies and PCR sequencing of the ITS1 region of the rDNA gene. Homology search showed 100 % similarity with T. interdigitale (GenBank accession number: KC595993), Arthroderma vanbreuseghemii (GenBank accession number: JQ407190) and zoophilic strain of T. interdigitale (GenBank accession number: AY062119.1.). Four weeks of oral and local treatment with itraconazole (100 mg twice a day) and fluconazole 0.3 % (eyedrops) induced complete remission. To our knowledge, this is the first report of tinea blepharo-ciliaris associated with tinea barbae in Romania.
KeywordsTinea blepharo-ciliaris Tinea barbae Trichophyton interdigitale (zoophilic) Sequencing
We are grateful to Dr. Orban Francisc for sending us this case. We would also like to thank Prof. Aurel Ardelean for his support. Written consent was obtained from the patient.
Compliance with Ethical Standards
Conflict of interest
The authors declare that they have no conflict of interest.
All procedures performed in this study involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.
Informed consent was obtained from the patient who participated in this study.
- 1.Hay RJ, Ashbee HR. Mycology. In: Burns T, Breathnach S, Cox N, Griffiths C, editors. Rook’s textbook of dermatology. 8th ed, vol 36. Oxford: Blackwell; 2010. p. 1–93.Google Scholar
- 2.Baran W, Szepietowski JC, Schwartz RA. Tinea barbae. Acta Dermatoven APA. 2004;13(3):91–4.Google Scholar
- 4.Sabouraud R. Les teignes. Paris: Masson; 1910.Google Scholar
- 7.Heidemann S, Monod M, Gräser Y. Signature polymorphisms in the internal transcribed spacer region relevant for the differentiation of zoophilic and anthropophilic strains of Trichophyton interdigitale and other species of T. mentagrophytes sensu lato. Br J Dermatol. 2010;162:282–95.CrossRefPubMedGoogle Scholar
- 9.Oyeka CA. Trichophyton mentagrophytes a keratinophilic fungus. Revista Iberoamericana de Micología Apdo. 2000:699, E-48080 Bilbao.Google Scholar
- 11.Kane J, Summerbell R, Sigler L, Krajden S, Land G. Laboratory handbook of dermatophytes: a clinical guide and laboratory manual of dermatophytes and other filamentous fungi from skin, hair and nails. Belmont: Star Publishing Company; 1997.Google Scholar