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Mycopathologia

, Volume 180, Issue 5–6, pp 403–406 | Cite as

Sequence-Based Identification of a Zoophilic Strain of Trichophyton interdigitale in a Rare Case of Tinea Blepharo-Ciliaris Associated with Tinea Barbae

  • Adrian M. Buruiana
  • Ciprian V. Mihali
  • Cristina PopescuEmail author
Article

Abstract

Impaired hair at blepharo-ciliaris area by dermatophytes is a rare clinical entity. This infection is often misdiagnosed or underdiagnosed, being mistakenly referred to as an infection of bacterial origin. Herein, we present a rare case of tinea blepharo-ciliaris associated with tinea barbae in an adult male. Considering the two lesions of the patient, mycological examination was performed by phenotypic methods, including environmental electronic scanning microscopy. Trichophyton interdigitale zoophilic strain was identified as the etiological agent by direct examination of the hair, primary culture analysis of the developed colonies and PCR sequencing of the ITS1 region of the rDNA gene. Homology search showed 100 % similarity with T. interdigitale (GenBank accession number: KC595993), Arthroderma vanbreuseghemii (GenBank accession number: JQ407190) and zoophilic strain of T. interdigitale (GenBank accession number: AY062119.1.). Four weeks of oral and local treatment with itraconazole (100 mg twice a day) and fluconazole 0.3 % (eyedrops) induced complete remission. To our knowledge, this is the first report of tinea blepharo-ciliaris associated with tinea barbae in Romania.

Keywords

Tinea blepharo-ciliaris Tinea barbae Trichophyton interdigitale (zoophilic) Sequencing 

Notes

Acknowledgments

We are grateful to Dr. Orban Francisc for sending us this case. We would also like to thank Prof. Aurel Ardelean for his support. Written consent was obtained from the patient.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in this study involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from the patient who participated in this study.

Supplementary material

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Adrian M. Buruiana
    • 1
  • Ciprian V. Mihali
    • 2
    • 3
  • Cristina Popescu
    • 2
    • 3
    Email author
  1. 1.Faculty of Natural Sciences, Engineering and Computer SciencesVasile Goldis Western University of AradAradRomania
  2. 2.Institute of Life SciencesVasile Goldis Western University of AradAradRomania
  3. 3.Faculty of Medicine, Pharmacy and Dental MedicineVasile Goldis Western University of AradAradRomania

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