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Mycopathologia

, Volume 179, Issue 1–2, pp 53–62 | Cite as

Isolation and Drug Susceptibility of Candida parapsilosis Sensu Lato and other Species of C. parapsilosis Complex from Patients with Blood Stream Infections and Proposal of a Novel LAMP Identification Method for the Species

  • Plinio Trabasso
  • Tetsuhiro Matsuzawa
  • Renata Fagnani
  • Yasunori Muraosa
  • Kenichiro Tominaga
  • Mariangela Ribeiro Resende
  • Katsuhiko Kamei
  • Yuzuru Mikami
  • Angelica Zaninelli Schreiber
  • Maria Luiza Moretti
Article

Abstract

Candida parapsilosis complex (CPC) is the third Candida species isolated in blood cultures of patients from our Hospital, following C. albicans and C. tropicalis. From 2006 to 2010, the median annual distribution of CPC was 8 cases/year. Records of 36 patients were reviewed. CPC were 31 (86.1 %) C. parapsilosis; 4 (11.1 %) C. orthopsilosis; and 1 (2.8 %) C. metapsilosis. Clinical characteristics were central venous catheter, 34 (94.4 %); parental nutrition, 25 (70 %); surgery, 27 (57.9 %); prior bacteremia, 20 (51.3 %); malignancy, 18 (50 %). General mortality was 47.2 %. Death was higher in immunosuppressed patients (17 vs. 11; p = 0.003). Three out four (75 %) patients with C. orthopsilosis and 14 out 31 (45.2 %) with C. parapsilosis died (p = 0.558). Thirty-nine individual isolates were tested for susceptibility to seven antifungal drugs, with MICs values showing susceptibility to all of them. Two isolates, one C. orthopsilosis and one C. parapsilosis, had fluconazole MIC = 4 μg/mL. Differentiation among CPC has implication in caring for patients with invasive candidiasis since there are differences in virulence, pathogenicity and drug susceptibility. A method targeting the topoisomerase II gene based on loop-mediated isothermal amplification (LAMP) was developed. LAMP emerges as a promising tool for the identification of fungal species due to the high sensitivity and specificity. LAMP can be performed at the point-of-care, being no necessary the use of expensive equipment. In our study, the method was successful comparing to the DNA sequencing and proved to be a reliable and fast assay to distinguish the three species of CPC.

Keywords

Candida parapsilosis Candidemia Mycoses Antifungal susceptibility Molecular diagnostic techniques LAMP 

Notes

Acknowledgments

This project was approved on February 22, 2011 (No. 039/2011) by the Ethical Committee of the School of Medical Sciences of the University of Campinas. This study was partly supported by JICA (Japan International Cooperation Agency) and SATREPS (Science and Technology Research Partnership for Sustainable Development) Grant No. 02P-29548-09.

Conflict of interest

All authors declare no conflict of interests.

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Plinio Trabasso
    • 1
  • Tetsuhiro Matsuzawa
    • 2
  • Renata Fagnani
    • 1
  • Yasunori Muraosa
    • 3
  • Kenichiro Tominaga
    • 3
  • Mariangela Ribeiro Resende
    • 1
  • Katsuhiko Kamei
    • 3
  • Yuzuru Mikami
    • 3
  • Angelica Zaninelli Schreiber
    • 4
  • Maria Luiza Moretti
    • 1
  1. 1.Infectious Diseases Division, School of Medical SciencesUniversity of CampinasCampinasBrazil
  2. 2.University of NagasakiNagasakiJapan
  3. 3.Medical Mycology Research CenterChiba UniversityChuokuJapan
  4. 4.Department of Clinical Pathology, School of Medical SciencesUniversity of CampinasCampinasBrazil

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