Mycopathologia

, Volume 176, Issue 5–6, pp 319–328 | Cite as

Siderophore Production by Pathogenic Mucorales and Uptake of Deferoxamine B

  • Gérald Larcher
  • Marylène Dias
  • Bienvenue Razafimandimby
  • Danielle Bomal
  • Jean-Philippe Bouchara
Article

Abstract

Clinical reports have established that mucormycosis, mainly caused by Rhizopus spp., frequently occurs in patients treated with deferoxamine B (DFO, Desferal®) which is misappropriated by these fungi. Siderophore production by twenty mucoralean isolates was therefore investigated using a commercial iron-depleted culture medium. Siderophore production was detected for most of the isolates. Our experiments confirmed that feroxamine B (iron chelate of DFO) promoted in vitro growth of Rhizopus arrhizus. Electrophoretic analysis of somatic extracts revealed iron-regulated proteins of 60 and 32 kDa which were lacking in iron-depleted culture conditions. Using a fluorescent derivative of deferoxamine B, we showed by fluorescence microscopy the entry of the siderophore within the fungal cells, thus suggesting a shuttle mechanism encompassing the uptake of the entire siderophore-ion complex into the cell. This useful tool renders possible a better understanding of iron metabolism in Mucorales which could lead to the development of new diagnostic method or new antifungal therapy using siderophores as imaging contrast agents or active drug vectors.

Keywords

Mucorales Rhizopus arrhizus Siderophore Fluorescent derivative of deferoxamine B 

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Gérald Larcher
    • 1
  • Marylène Dias
    • 2
  • Bienvenue Razafimandimby
    • 1
  • Danielle Bomal
    • 1
  • Jean-Philippe Bouchara
    • 1
    • 3
  1. 1.Groupe d’Etude des Interactions Hôte-Pathogène, UPRES-EA 3142, Institut de Biologie en Santé, IRIS, Centre Hospitalier UniversitaireL’UNAM Université, Université d’AngersAngers CédexFrance
  2. 2.Laboratoire MOLTECH-Anjou, UMR CNRS 6200L’UNAM Université, Université d’AngersAngersFrance
  3. 3.Laboratoire de Parasitologie-Mycologie, Institut de Biologie en Santé, PBHCentre Hospitalier UniversitaireAngersFrance

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