Microsporum fulvum, an Ignored Pathogenic Dermatophyte: A New Clinical Isolation from Iran
- 307 Downloads
Misidentifying with Microsporum gypseum has for a long time been accounted for less prevalence of the geophilic species, Microsporum fulvum in human dermatophytosis. We describe a new case of infection with the species in an Iranian young man. Direct examination of skin scrapings revealed a tinea corporis, and morphological study of the recovered isolate from the culture resulted in the identification of M. gypseum. However, PCR amplification of ITS1-5.8S rDNA-ITS2 region and subsequent ITS-RFLP and sequencing were indicative of M. fulvum as the true causative agent. To recognize M. fulvum in human infections and to validate the morphologically distinguished isolates of M. gypseum, the genetic-based identification is strongly recommended.
KeywordsMicrosporum fulvum Dermatophytosis Tinea corporis ITS
- 1.Ellis D, Davis S, Alexious H, Handke R, Bartely R. Descriptions of medical fungi. 2nd ed. North Adelaide: Mycology Unit, Women’s and Children’s Hospital; 2007. p. 92–4.Google Scholar
- 6.De HooG GS, Guarro J, Gene J, Figueras MJ. Atlas of clinical fungi. 2nd ed. Utrecht: Centraalbureau voor Schimmelcultures; 2000.Google Scholar
- 11.White TJ, Bruns T, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, editor. PCR protocols: a guide to methods and applications. London: Academic Press; 1990. p. 315–22.Google Scholar
- 12.Rezaei-Matehkolaei A, Makimura K, Shidfar MR, Zaini F, Eshraghian MR, Jalalizand N, Nouripour-Sisakht S, Mirhendi H. Use of single-enzyme PCR-restriction digestion barcode targeting the internal transcribed spacers (ITS rDNA) to identify dermatophyte species. Iran J Public Health. 2012;41(3):82–94.PubMedGoogle Scholar