, Volume 176, Issue 1–2, pp 157–160 | Cite as

Microsporum fulvum, an Ignored Pathogenic Dermatophyte: A New Clinical Isolation from Iran

  • Sadegh Nouripour-Sisakht
  • Ali Rezaei-MatehkolaeiEmail author
  • Mahdi Abastabar
  • Mohammad Javad Najafzadeh
  • Kazuo Satoh
  • Bahram Ahmadi
  • Leila Hosseinpour


Misidentifying with Microsporum gypseum has for a long time been accounted for less prevalence of the geophilic species, Microsporum fulvum in human dermatophytosis. We describe a new case of infection with the species in an Iranian young man. Direct examination of skin scrapings revealed a tinea corporis, and morphological study of the recovered isolate from the culture resulted in the identification of M. gypseum. However, PCR amplification of ITS1-5.8S rDNA-ITS2 region and subsequent ITS-RFLP and sequencing were indicative of M. fulvum as the true causative agent. To recognize M. fulvum in human infections and to validate the morphologically distinguished isolates of M. gypseum, the genetic-based identification is strongly recommended.


Microsporum fulvum Dermatophytosis Tinea corporis ITS 


  1. 1.
    Ellis D, Davis S, Alexious H, Handke R, Bartely R. Descriptions of medical fungi. 2nd ed. North Adelaide: Mycology Unit, Women’s and Children’s Hospital; 2007. p. 92–4.Google Scholar
  2. 2.
    Seyfarth F, Goetze S, Erhard M, Burmester A, Elsner P, Hipler UC. Infection with a rare geophilic dermatophyte. Hautarzt. 2010;61:694–9.PubMedCrossRefGoogle Scholar
  3. 3.
    Herpay Z, Pintye I, Rieth H. Favus-like fungal disease of the scalp caused by Microsporum fulvum, Uriburu 1909 (Nannizzia fulva, Stockdale 1963), in a 3-year-old girl. Mykosen. 1973;16:333–4.PubMedCrossRefGoogle Scholar
  4. 4.
    Elewski BE. Tinea capitis: a current perspective. J Am Acad Dermatol. 2000;42:1–20.PubMedCrossRefGoogle Scholar
  5. 5.
    Del Boz J, Crespo V, Rivas-Ruiz F, De Troya M. Tinea incognito in children: 54 cases. Mycoses. 2011;54:254–8.PubMedCrossRefGoogle Scholar
  6. 6.
    De HooG GS, Guarro J, Gene J, Figueras MJ. Atlas of clinical fungi. 2nd ed. Utrecht: Centraalbureau voor Schimmelcultures; 2000.Google Scholar
  7. 7.
    Rezaei-Matehkolaei A, Makimura K, de Hoog GS, Shidfar MR, Satoh K, Najafzadeh MJ, Mirhendi H. Multilocus differentiation of the related dermatophytes Microsporum canis, Microsporum ferrugineum and Microsporum audouinii. J Med Microbiol. 2012;61:57–63.PubMedCrossRefGoogle Scholar
  8. 8.
    Rezaei-Matehkolaei A, Makimura K, De Hoog GS, Shidfar MR, Satoh K, Najafzadeh MJ, Mirhendi H. Discrimination of Trichophyton tonsurans and Trichophyton equinum by PCR-RFLP and by β-tubulin and Translation Elongation Factor 1-α sequencing. Med Mycol. 2012;50:760–4.PubMedCrossRefGoogle Scholar
  9. 9.
    Rezaei-Matehkolaei A, Makimura K, de Hoog S, Shidfar MR, Zaini F, Eshraghian M, Adimi Naghan P, Mirhendi H. Molecular epidemiology of dermatophytosis in Tehran, Iran, a clinical and microbial survey. Med Mycol. 2013;51:203–7.PubMedCrossRefGoogle Scholar
  10. 10.
    Weitzman I, Padhye AA. Dermatophytes: gross and microscopic. Dermatol Clin. 1996;14:9–22.PubMedCrossRefGoogle Scholar
  11. 11.
    White TJ, Bruns T, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, editor. PCR protocols: a guide to methods and applications. London: Academic Press; 1990. p. 315–22.Google Scholar
  12. 12.
    Rezaei-Matehkolaei A, Makimura K, Shidfar MR, Zaini F, Eshraghian MR, Jalalizand N, Nouripour-Sisakht S, Mirhendi H. Use of single-enzyme PCR-restriction digestion barcode targeting the internal transcribed spacers (ITS rDNA) to identify dermatophyte species. Iran J Public Health. 2012;41(3):82–94.PubMedGoogle Scholar
  13. 13.
    Farag A, Taha M, Halim S. One-week therapy with oral terbinafine in cases of tinea cruris/corporis. Br J Dermatol. 1994;131:684–6.PubMedCrossRefGoogle Scholar
  14. 14.
    Nenoff P, Erhard M, Simon JC, Muylowa GK, Herrmann J, Rataj W, Graser Y. MALDI-TOF mass spectrometry-a rapid method for the identification of dermatophyte species. Med Mycol. 2013;51:17–24.PubMedCrossRefGoogle Scholar
  15. 15.
    Weitzman I, Summerbell RC. The dermatophytes. Clin Microbiol Rev. 1995;8:240–59.PubMedGoogle Scholar
  16. 16.
    Li HC, Bouchara JP, Hsu MML, Barton R, Chang TC. Identification of dermatophytes by an oligonucleotide array. J Clin Microbiol. 2007;45:3160–6.PubMedCrossRefGoogle Scholar
  17. 17.
    Kawasaki M, Ishizaki H, Aoki M, Watanabe S. Phylogeny of Nannizzia incurvata, N. gypsea, N. fulva and N. otae by restriction enzyme analysis of mitochondrial DNA. Mycopathologia. 1990;112:173–7.PubMedCrossRefGoogle Scholar
  18. 18.
    Sinski JT, Flouras K. A survey of dermatophytes isolated from human patients in the United States from 1979 to 1981 with chronological listings of worldwide incidence of five dermatophytes often isolated in the United States. Mycopathologia. 1984;85:97–120.PubMedCrossRefGoogle Scholar
  19. 19.
    Demange C, Cotet-Audonneau N, Kombila M, Miegeville M, Berthonneau M, De Vroey C, Percebois G. Microsporum gypseum complex in man and animals. Med Mycol. 1992;30:301–38.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Sadegh Nouripour-Sisakht
    • 1
  • Ali Rezaei-Matehkolaei
    • 2
    Email author
  • Mahdi Abastabar
    • 1
  • Mohammad Javad Najafzadeh
    • 3
  • Kazuo Satoh
    • 4
  • Bahram Ahmadi
    • 1
  • Leila Hosseinpour
    • 1
  1. 1.Department of Medical Parasitology and Mycology, School of Public HealthTehran University of Medical SciencesTehranIran
  2. 2.Department of Medical Mycology, School of MedicineAhvaz Jundishapur University of Medical SciencesAhvazIran
  3. 3.Department of Parasitology and Mycology, Ghaem Hospital, School of MedicineMashhad University of Medical SciencesMashhadIran
  4. 4.Teikyo University Institute of Medical MycologyHachiojiJapan

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