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Mycopathologia

, Volume 175, Issue 3–4, pp 281–286 | Cite as

Antifungal Activity of Nanocapsule Suspensions Containing Tea Tree Oil on the Growth of Trichophyton rubrum

  • F. C. Flores
  • J. A. de Lima
  • R. F. Ribeiro
  • S. H. Alves
  • C. M. B. Rolim
  • R. C. R. Beck
  • Cristiane Bona da Silva
Article

Abstract

The aim of this study was to evaluate, for the first time, the antifungal efficacy of nanocapsules and nanoemulsions containing Melaleuca alternifolia essential oil (tea tree oil) in an onychomycosis model. The antifungal activity of nanostructured formulations was evaluated against Trichophyton rubrum in two different in vitro models of dermatophyte nail infection. First, nail powder was infected with T. rubrum in a 96-well plate and then treated with the formulations. After 7 and 14 days, cell viability was verified. The plate counts for the samples were 2.37, 1.45 and 1.0 log CFU mL−1 (emulsion, nanoemulsion containing tea tree oil and nanocapsules containing tea tree oil, respectively). A second model employed nails fragments which were infected with the microorganism and treated with the formulations. The diameter of fungal colony was measured. The areas obtained were 2.88 ± 2.08 mm2, 14.59 ± 2.01 mm2, 40.98 ± 2.76 mm2 and 38.72 ± 1.22 mm2 for the nanocapsules containing tea tree oil, nanoemulsion containing tea tree oil, emulsion and untreated nail, respectively. Nail infection models demonstrated the ability of the formulations to reduce T. rubrum growth, with the inclusion of oil in nanocapsules being most efficient.

Keywords

Nanoemulsions Nanocapsules Tea tree oil Trichophyton rubrum Onychomycosis 

Notes

Acknowledgments

The authors thank to CNPq-Brasília/Brazil for financial support.

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • F. C. Flores
    • 1
  • J. A. de Lima
    • 2
  • R. F. Ribeiro
    • 1
  • S. H. Alves
    • 1
  • C. M. B. Rolim
    • 1
  • R. C. R. Beck
    • 3
  • Cristiane Bona da Silva
    • 1
    • 2
  1. 1.Programa de Pós-Graduação em Ciências FarmacêuticasUniversidade Federal de Santa MariaSanta MariaBrazil
  2. 2.Departamento de Farmácia Industrial, Curso de Farmácia, Centro de Ciências da SaúdeUniversidade Federal de Santa MariaSanta MariaBrazil
  3. 3.Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de FarmáciaUniversidade Federal do Rio Grande do SulPorto AlegreBrazil

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