, Volume 174, Issue 3, pp 215–221 | Cite as

Effects of Antifungal Agents Alone and in Combination Against Candida glabrata Strains Susceptible or Resistant to Fluconazole

  • Izabel Almeida Alves
  • Laíssa Arévalo Bandeira
  • Débora Alves Nunes MarioEmail author
  • Laura Bedin Denardi
  • Louise Vignoles Neves
  • Janio Morais Santurio
  • Sydney Hartz Alves


The rise of Candida spp. resistant to classic triazole antifungal agents has led to a search for new therapeutic options. Here, we evaluated combinations of antifungals in a checkerboard assay against two groups of Candida glabrata strains: one containing fluconazole-susceptible clinical isolates (FS) and another containing fluconazole-resistant laboratory derivative (FR). The most synergistic combination observed was amphotericin B + flucytosine (synergistic for 61.77 % of FS strains and 76.47 % of FR strains). The most antagonistic combination observed was ketoconazole + flucytosine (FS 61.77 % and FR 55.88 %). Surprisingly, most combinations evidenced indifferent interactions, and the best synergism appeared when amphotericin B and flucytosine were combined against both groups of isolates.


Candida glabrata Resistance Antifungal drug combination 


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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Izabel Almeida Alves
    • 1
  • Laíssa Arévalo Bandeira
    • 1
  • Débora Alves Nunes Mario
    • 1
    Email author
  • Laura Bedin Denardi
    • 1
  • Louise Vignoles Neves
    • 1
  • Janio Morais Santurio
    • 1
  • Sydney Hartz Alves
    • 1
  1. 1.Departamento de Microbiologia e ParasitologiaUniversidade Federal de Santa MariaSanta MariaBrazil

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