Mycopathologia

, Volume 173, Issue 1, pp 63–68 | Cite as

Serological Survey of Paracoccidioidomycosis in Sheep

  • Gabriela G. Oliveira
  • Italmar T. Navarro
  • Roberta L. Freire
  • Donizeti R. Belitardo
  • Luciane H. Silveira
  • Zoilo P. Camargo
  • Eiko N. Itano
  • Mario A. Ono
Article

Abstract

The objective of this study was to evaluate the prevalence of antibodies against Paracoccidioides brasiliensis in sheep from Guarapuava, Paraná State, Brazil. The seroepidemiological study was carried out in 262 sheep. The samples were analyzed by ELISA and immunodiffusion test using P. brasiliensis gp43 and exoantigen as antigens, respectively. Initially, two sheep were immunized with P. brasiliensis to evaluate whether contact with the fungal cells could induce a humoral immune response against gp43 and exoantigen from P. brasiliensis. Both animals produced antibodies against gp43 and exoantigen, the main antigens used for diagnosis and seroepidemiology of paracoccidioidomycosis. A reactivity of 37% was observed to the P. brasiliensis gp43 antigen by ELISA although no reactivity had been observed by the immunodiffusion test. Sheep under extensive grazing system showed higher frequency of positivity to P. brasiliensis (P ≤ 0.05) than those under intensive and semi-intensive systems. These data suggest that sheep may be a useful epidemiological marker of P. brasiliensis presence in the environment and reinforce that contact with soil is an important risk factor for infection.

Keywords

Paracoccidioides brasiliensis Epidemiology Seroepidemiology 

Notes

Acknowledgments

The authors thank the CNPq and CAPES for financial support and CNPq for the productivity fellowship granted to MA Ono, EN Itano, IT Navarro and ZP Camargo.

References

  1. 1.
    Lutz A. Uma micose pseudo-coccidica localizada na boca e observada no Brasil: contribuição ao conhecimento das hypho-blastomycoses americanas. Bras Med. 1908;22:141–4.Google Scholar
  2. 2.
    Brummer E, Castañeda E, Restrepo A. Paracoccidioidomycosis: an update. Clin Microbiol Rev. 1993;6:89–117.PubMedGoogle Scholar
  3. 3.
    Restrepo A, McEwen JG, Castañeda E. The habitat of Paracoccidioides brasiliensis: how far from solving the riddle? Med Mycol. 2001;39:233–41.PubMedGoogle Scholar
  4. 4.
    Negroni P. El Paracoccidioides brasiliensis vive saprofiticamente en el suelo argentino. Prensa Méd Argent. 1966;53:2831–2.Google Scholar
  5. 5.
    Albornoz MB. Isolation of Paracoccidioides brasiliensis from rural soil in Venezuela. Sabouraudia. 1971;9:248–53.PubMedCrossRefGoogle Scholar
  6. 6.
    Silva-Vergara ML, Martinez R, Chadu A, Madeira M, Freitas-Silva G, Maffei CML. Isolation of Paracoccidioides brasiliensis strain from the soil of a coffee plantation in Ibiá, State of Minas Gerais, Brazil. Med Mycol. 1998;36:37–42.PubMedCrossRefGoogle Scholar
  7. 7.
    Conti-Diaz IA, Rilla FD. Hipótesis sobre el nicho ecológico de Paracoccidioides brasiliensis. Rev Méd Urug. 1989;5:97–103.Google Scholar
  8. 8.
    McEwen JG, Garcia AM, Ortiz BL, Botero S, Restrepo A. In search of the natural habitat of Paracoccidioides brasiliensis. Arch Med Res. 1995;26:305–6.PubMedGoogle Scholar
  9. 9.
    Terçarioli GR, Bagagli E, Reis GM, Theodoro RC, Bosco SMG, Macoris SAG, Pereira VBR. Ecological study of Paracoccidioides brasiliensis in soil: growth ability, conidia production and molecular detection. BMC Microbiol. 2007;7:92.PubMedCrossRefGoogle Scholar
  10. 10.
    Restrepo A. The ecology of Paracoccidioides brasiliensis: a puzzle still unsolved. Sabouraudia. 1985;23:323–34.PubMedCrossRefGoogle Scholar
  11. 11.
    Ono MA, Itano EN, Mizuno LT, Mizuno EH, Camargo ZP. Inhibition of Paracoccidioides brasiliensis by pesticides: is this a partial explanation for the difficulty in isolating this fungus from the soil? Med Mycol. 2002;40:493–9.PubMedGoogle Scholar
  12. 12.
    Mós EN, Fava-Netto C. Contribuição ao estudo da paracoccidoidomicose I—Possível papel dos cães: Estudo sorológico e anatomo-patológico. Rev Inst Med Trop São Paulo. 1974;16:154–9.Google Scholar
  13. 13.
    Ono MA, Bracarense APFRL, Morais HSA, Trapp SM, Belitardo DR, Camargo ZP. Canine paracoccidioidomycosis: a seroepidemiologic study. Med Mycol. 2001;39:277–82.PubMedGoogle Scholar
  14. 14.
    Silveira LH, Domingos IH, Kouchi K, Itano EN, Silva EA, Landgraf VO, Werneck SM, Camargo ZP, Ono MA. Serological detection of antibodies against Paracoccidioides brasiliensis in dogs with leishmaniasis. Mycopathologia. 2006;162:325–9.PubMedCrossRefGoogle Scholar
  15. 15.
    Fagundes RQ, Araújo Jr JP, Modolo R, Bagagli E. Serological detection of Paracoccidioidomycosis in dogs from the endemic area of Botucatu-SP, Brazil. ARBS, Pirenópolis-GO, Brasil, 2002.Google Scholar
  16. 16.
    Canteros CE, Madariaga MJ, Lee W, Rivas MC, Davel G, Iachini R. Endemic fungal pathogens in a rural setting of Argentina: seroepidemiological study in dogs. Rev Iberoam Micol. 2010;27(1):14–9.PubMedCrossRefGoogle Scholar
  17. 17.
    Fontana FF, Santos CTB, Esteves FM, Rocha A, Fernandes GF, Amaral CC, Domingues MA, Camargo ZP, Silva-Vergara ML. Seroepidemiological survey of paracoccidioidomycosis infection among urban and rural dogs from Uberaba, Minas Gerais, Brazil. Mycopathologia. 2010;169:159–65.PubMedCrossRefGoogle Scholar
  18. 18.
    Conti-Diaz IA, Alvarez BJ, Gezuele E, Gonzalez MH, Duarte J, Falcon J. Intradermal reaction survey with paracoccidioidin and histoplasmin in horses. Rev Inst Med Trop São Paulo. 1972;14:372–6.PubMedGoogle Scholar
  19. 19.
    Corte AC, Itano EN, Freire RL, Camargo ZP, Ono MA. Detection of antibodies to Paracoccidioides brasiliensis in horses from northern Region of Paraná State. Semina-Ciências Agrárias. 2009;30:441–6.Google Scholar
  20. 20.
    Costa EO, Fava-Netto C. Contribution to the epidemiology of paracoccidioidomycosis and histoplasmosis in the State of São Paulo, Brazil. Paracoccidioidin and histoplasmin intradermic tests in domestic animals. Sabouraudia. 1978;16:93–101.PubMedCrossRefGoogle Scholar
  21. 21.
    Costa EO, Diniz LS, Netto F, Arruda C, Dagli ML. Delayed hypersensitivity test with paracoccidioidin in captive Latin American wild mammals. J Med Vet Mycol. 1995;33:39–42.PubMedCrossRefGoogle Scholar
  22. 22.
    Gutierrez AH, Ceballos G, Ferrer HI, Rangel O. Encuesta sobre tuberculosis, histoplasmosis y paracoccidioidomicosis en ganado lechero del Valle del Aburra. Antioquia Medica. 1974;24:339–58.Google Scholar
  23. 23.
    Silveira LH, Paes RCS, Medeiros EV, Itano EM, Camargo ZP, Ono MA. Occurrence of antibodies to Paracoccidioides brasiliensis in dairy cattle from Mato Grosso do Sul, Brazil. Mycopathologia. 2008;165:367–71.PubMedCrossRefGoogle Scholar
  24. 24.
    Oliveira GG, Silveira, LH, Itano EN, Soares RM, Freire RF, Watanabe MAE, Ono MA. Serological Evidence of Paracoccidioides brasiliensis in Chicken from Paraná and Mato Grosso do Sul States, Brazil. Mycopathologia 2011;171:197–202.Google Scholar
  25. 25.
    Fernandes GF, Deps P, Tomimori-Yamashita J, Camargo ZP. IgM and IgG antibody response to Paracoccidioides brasiliensis in naturally infected wild armadillos (Dasypus novemcinctus). Med Mycol. 2004;42:363–8.PubMedCrossRefGoogle Scholar
  26. 26.
    Corte AC, Svoboda WK, Navarro IT, Freire RL, Malanski LS, Shiozawa MM, Ludwig G, Aguiar LM, Passos FC, Maron A, Camargo ZP, Itano EN, Ono MA. Paracoccidioidomycosis in wild monkeys from Paraná State, Brazil. Mycopathologia. 2007;164:225–8.PubMedCrossRefGoogle Scholar
  27. 27.
    Richini-Pereira VB, Bosco SM, Griese J, Theodoro RC, Macoris SA, Silva RJ, Barrozo L, Tavares PM, Zancopé-Oliveira RM, Bagagli E. Molecular detection of Paracoccidioides brasiliensis in road-killed wild animals. Med Mycol. 2008;46:35–40.PubMedCrossRefGoogle Scholar
  28. 28.
    Naiff RD, Ferreira LCL, Barret TV, Naif MF, Arias JR. Paracoccidioidomicose enzoótica em tatus (Dasypus novemcinctus) no estado do Pará. Rev Inst Med Trop São Paulo. 1986;28:19–27.PubMedGoogle Scholar
  29. 29.
    Bagagli E, Sano A, Coelho KI, Alquati S, Miyaji M, Camargo ZP, Gomes GM, Franco M, Montenegro MR. Isolation of Paracoccidioides brasiliensis from armadillos (Dasypus novemcinctus) captured in an area of paracoccidioidomycosis. Am J Trop Med Hyg. 1998;58:505–12.PubMedGoogle Scholar
  30. 30.
    Corredor GG, Castaño JH, Peralta LA, Díez S, Arango M, Mcwen J, Restrepo A. Isolation of Paracoccidioides brasiliensis from the nine-banded armadillo Dasypus novemcinctus, in an endemic area for paracoccidioidomycosis in Colombia. Rev Iberoam Micol. 1999;16:216–20.PubMedGoogle Scholar
  31. 31.
    Gezuele E. Aislamiento de Paracoccidioides brasiliensis de heces de pinguino de la Antardida. IV Encuentro Internacional sobre Paracoccidioidomicosis, Caracas. Venezuela, Instituto Venezuelano de Investigaciones Cientificas (IVIC): 1989 (Abstract B-2).Google Scholar
  32. 32.
    Farias MR, Condas LAZ, Ribeiro MG, Bosco SMG, Muro MD, Werner J, Theodoro RC, Bagagli E, Marques SA, Franco M. Paracoccidioidomycosis in a dog: case report of generalized lymphadenomegaly. Mycopathologia. 2011;172:147–52.PubMedCrossRefGoogle Scholar
  33. 33.
    Gonzales JF, Montiel NA, Maass RL. First report on the diagnosis of encephalic and urinary paracoccidioidomycosis in a cat. J Fel Med Surg. 2010;12:659–62.CrossRefGoogle Scholar
  34. 34.
    Trejo-Chávez A, Ramírez-Romero R, Ancer-Rodríguez J, Nevárez-Garza AM, Rodríguez-Tovar E. Disseminated Paracoccidioidomycosis in a Southern Two-Toed Sloth (Choloepus didactylus). J Comp Pathol. 2011;144:231–4.PubMedCrossRefGoogle Scholar
  35. 35.
    Bittencourt JI, de Oliveira RM, Coutinho ZF. Paracoccidioidomycosis mortality in the State of Paraná, Brazil, 1980/1998. Cad Saude Publica. 2005;21:1856–64.PubMedCrossRefGoogle Scholar
  36. 36.
    Camargo ZP, Unterkircher CS, Campoy SP, Travassos LR. Production of Paracoccidioides brasiliensis exoantigens for immunodiffusion tests. J Clin Microbiol. 1988;26:2147–51.PubMedGoogle Scholar
  37. 37.
    Puccia R, Travassos LR. The 43-kDa glycoprotein from the human pathogen Paracoccidioides brasiliensis and its deglycosylated form: excretion and susceptibility to proteolysis. Arch Biochem Biophys. 1991;289:298–302.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Gabriela G. Oliveira
    • 1
  • Italmar T. Navarro
    • 2
  • Roberta L. Freire
    • 2
  • Donizeti R. Belitardo
    • 1
  • Luciane H. Silveira
    • 3
  • Zoilo P. Camargo
    • 4
  • Eiko N. Itano
    • 1
  • Mario A. Ono
    • 1
  1. 1.Departamento de Ciências Patológicas, Centro de Ciências BiológicasUniversidade Estadual de LondrinaLondrinaBrazil
  2. 2.Departamento de Medicina Veterinária PreventivaUniversidade Estadual de LondrinaLondrinaBrazil
  3. 3.Departamento de Patologia Geral, Faculdades Luiz MeneghelBandeirantesParanáBrazil
  4. 4.Escola Paulista de MedicinaUniversidade Federal de São Paulo, UNIFESPSão PauloBrazil

Personalised recommendations