Mycopathologia

, 172:331

Transcriptomic and Proteomic Profile of Aspergillus fumigatus on Exposure to Artemisinin

  • Poonam Gautam
  • Santosh Kumar Upadhyay
  • Wazid Hassan
  • Taruna Madan
  • Ravi Sirdeshmukh
  • Curam Sreenivasacharlu Sundaram
  • Wasudev Namdeo Gade
  • Seemi Farhat Basir
  • Yogendra Singh
  • Puranam Usha Sarma
Article

DOI: 10.1007/s11046-011-9445-3

Cite this article as:
Gautam, P., Upadhyay, S.K., Hassan, W. et al. Mycopathologia (2011) 172: 331. doi:10.1007/s11046-011-9445-3

Abstract

Artemisinin, an antimalarial drug, and its derivatives are reported to have antifungal activity against some fungi. We report its antifungal activity against Aspergillus fumigatus (A. fumigatus), a pathogenic filamentous fungus responsible for allergic and invasive aspergillosis in humans, and its synergistic effect in combination with itraconazole (ITC), an available antifungal drug. In order to identify its molecular targets, we further analyzed transcript and proteomic profiles of the fungus on exposure to the artemisinin. In transcriptomic analysis, a total of 745 genes were observed to be modulated on exposure to artemisinin, and some of them were confirmed by real-time polymerase chain reaction analysis. Proteomic profiles of A. fumigatus treated with artemisinin showed modulation of 175 proteins (66 upregulated and 109 downregulated) as compared to the control. Peptide mass fingerprinting led to the identification of 85 proteins—29 upregulated and 56 downregulated, 65 of which were unique proteins. Consistent with earlier reports of molecular mechanisms of artemisinin and that of other antifungal drugs, we believe that oxidative phosphorylation pathway (64 kDa mitochondrial NADH dehydrogenase), cell wall-associated proteins and enzymes (conidial hydrophobin B protein, cell wall phiA protein, extracellular thaumatin domain protein, 1,3-beta-glucanosyltransferase Gel2) and genes involved in ergosterol biosynthesis (ERG6 and coproporphyrinogen III oxidase, HEM13) are potential targets of artemisinin for further investigations.

Keywords

Aspergillus fumigatus Artemisinin Microarray Proteomics 

Supplementary material

11046_2011_9445_MOESM1_ESM.doc (36 kb)
Supplementary material 1 (DOC 36 kb)
11046_2011_9445_MOESM2_ESM.xls (126 kb)
Supplementary material 2 (XLS 126 kb)
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Supplementary material 3 (XLS 48 kb)
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Supplementary material 4 (XLS 62 kb)
11046_2011_9445_MOESM5_ESM.doc (82 kb)
Supplementary material 5 (DOC 82 kb)
11046_2011_9445_MOESM6_ESM.ppt (152 kb)
Supplementary material 6 (PPT 152 kb)

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Poonam Gautam
    • 1
    • 2
    • 4
  • Santosh Kumar Upadhyay
    • 1
    • 5
  • Wazid Hassan
    • 1
  • Taruna Madan
    • 1
    • 3
  • Ravi Sirdeshmukh
    • 2
  • Curam Sreenivasacharlu Sundaram
    • 2
  • Wasudev Namdeo Gade
    • 4
  • Seemi Farhat Basir
    • 5
  • Yogendra Singh
    • 1
  • Puranam Usha Sarma
    • 1
    • 6
  1. 1.Institute for Genomics and Integrative BiologyDelhiIndia
  2. 2.Centre for Cellular and Molecular BiologyHyderabadIndia
  3. 3.Innate ImmunityNational Institute for Research in Reproductive Health (NIRRH)MumbaiIndia
  4. 4.Department of BiotechnologyUniversity of PunePuneIndia
  5. 5.Department of BiosciencesJamia Milia IslamiaDelhiIndia
  6. 6.Department of Plant PathologyIndian Agricultural Research InstituteDelhiIndia

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