Advertisement

Mycopathologia

, Volume 172, Issue 2, pp 147–152 | Cite as

Paracoccidioidomycosis in a Dog: Case Report of Generalized Lymphadenomegaly

  • Marconi Rodrigues de Farias
  • Larissa Anuska Zeni Condas
  • Márcio Garcia Ribeiro
  • Sandra de Moraes Gimenes BoscoEmail author
  • Marisol Dominguez Muro
  • Juliana Werner
  • Raquel Cordeiro Theodoro
  • Eduardo Bagagli
  • Sílvio Alencar Marques
  • Marcello Franco
Article

Abstract

Paracoccidioidomycosis (PCM) is a severe systemic mycosis, endemic in Latin America and highly prevalent in Brazil, where it ranks eighth as a mortality cause among infectious and parasitic diseases in humans. The disease in animals has been little explored. It is observed that armadillos can harbor the fungus at high frequencies, although the active disease has not been well documented in this wild mammal. Dogs are susceptible to experimental infection, and the naturally acquired PCM-disease was reported only recently in a dog from Brazil. The present work reports the second case of naturally acquired PCM in a 6-year-old female dog that presented emaciation, lymphadenomegaly, and hepatosplenomegaly. Biochemical and pulmonary radiographic evaluation did not reveal any abnormalities. PCM was diagnosed by clinical findings, culturing, immunohistochemistry, and histopathology of popliteal lymph node. The fungus was recovered from popliteal lymph node, and the molecular analysis showed respective sequencing similarities of 99 and 100% for 803 nucleotides of the Gp43 gene and 592 nucleotides from the ITS-5.8S region of Paracoccidioides brasiliensis. Immunohistochemistry revealed severe lymphadenitis and presented numerous yeasts, which reacted against the gp43 antibody. Histopathology revealed a severe granulomatous lymphadenitis associated with numerous single or multiple budding yeasts. After diagnosis, the dog was successfully treated with itraconazol for 2 years. Veterinarians should be aware of the importance of considering PCM for differential diagnosis, especially in dogs from PCM-endemic areas, whose monophagocytic system involvement is evident.

Keywords

Paracoccidioides brasiliensis Dog Lymphadenomegaly Granulomatous disease 

References

  1. 1.
    Untereiner WA, Scott JA, Naveau FA, et al. The Ajellomycetaceae, a new family of vertebrate-associated Onygenales. Mycologia. 2004;96:812–21.PubMedCrossRefGoogle Scholar
  2. 2.
    Wanke B, Londero AT. Epidemiology and paracoccidioidomycosis infection. In: Franco M, Lacaz CS, Restrepo A, Del Negro G, editors. Paracoccidioidomycosis. Boca Raton: CRS Press; 1994. p. 109–20.Google Scholar
  3. 3.
    Coutinho ZF, Silva D, Lazera M, et al. Paracoccidioidomycosis mortality in Brazil (1980–1995). Cad Saude Publica. 2002;18:1441–54.PubMedCrossRefGoogle Scholar
  4. 4.
    Franco M, Montenegro M, Mendes RP. Paracoccidioidomycosis: a recently proposed classification of its clinical forms. Rev Soc Bras Med Trop. 1987;20:129–32.PubMedGoogle Scholar
  5. 5.
    Shikanai-Yasuda MA, Telles Filho FQ, Mendes RP, et al. Consenso em Paracoccidioidomicose. Rev Soc Bras Med Trop. 2006;39:297–310.PubMedCrossRefGoogle Scholar
  6. 6.
    Costa EO, Diniz LSM, Fava-Netto C, et al. Delayed hypersensitivity test with paracoccidioidin in captive Latin American wild mammals. J Med Vet Mycol. 1995;33:39–42.PubMedCrossRefGoogle Scholar
  7. 7.
    Costa EO, Diniz LSM, Fava-Netto C. The prevalence of positive intradermal reactions to paracoccidioidin in domestic and wild animals in São Paulo. Brazil Vet Res Commun. 1995;19:127–30.CrossRefGoogle Scholar
  8. 8.
    Costa EO, Fava-Netto C. Contribution to the epidemiology of paracoccidioidomycosis and histoplasmosis in the State of São Paulo, Brazil. Paracoccidioidin and histoplasmin intradermic tests in domestic animals. Sabouraudia. 1978;16:93–101.PubMedCrossRefGoogle Scholar
  9. 9.
    Ono MA, Bracarense AP, Morais HS, Trapp SM, Belitardo DR, Camargo ZP. Canine paracoccidioidomycosis: a seroepidemiologic study. Med Mycol. 2001;39:277–82.PubMedGoogle Scholar
  10. 10.
    Bagagli E, Franco M, Bosco SMG, et al. High frequency of Paracoccidioides brasiliensis infection in armadillos (Dasypus novemcinctus): an ecology study. Med Mycol. 2003;41:217–23.PubMedCrossRefGoogle Scholar
  11. 11.
    Ricci G, Mota FT, Wakamatsu A, Sakamatsu A, Serafim RC, Franco M. Canine paracoccidioidomycosis. Med Mycol. 2004;42:379–83.PubMedCrossRefGoogle Scholar
  12. 12.
    Bosco SMG, Theodoro RC, Macoris SAG, et al. Morphological and molecular characterization of the first isolate of Paracoccidioides brasiliensis from dog (Canis familiaris) naturally infected. Rev Inst Med Trop São Paulo. 2005;47(suppl 14):62–3.Google Scholar
  13. 13.
    Eisele RC, Juliani RC, Belitardo DR, Itano EN, Estevão D, Bracarense APFRL, Camargo ZP, Ono MA. Immune response in dogs experimentally infected with Paracoccidioides brasiliensis. Med Mycol. 2004;42:549–53.PubMedCrossRefGoogle Scholar
  14. 14.
    Franco M, Bagagli E, Scapolio S, Lacaz CS. A critical analysis of isolation of Paracoccidioides brasiliensi from soil. Med Mycol. 2000;38:185–91.PubMedGoogle Scholar
  15. 15.
    Centers for Disease Control and Prevention (CDC). Outbreak of histoplasmosis among travelers returning from El Salvador-Pennsylvania and Virginia. Morb Mortal Wkly Rep. 2008;57:1349–1353.Google Scholar
  16. 16.
    Taboada J. Micoses Sistêmicas. In: Ettinger SJ, Feldman EC, editors. Tratado de medicina interna veterinária: doenças do cão e do gato. Rio de Janeiro: Guanabara Koogan; 2004. p. 478–503.Google Scholar
  17. 17.
    Morais FV, Barros TF, Kukada MK, Cisalpino PS, Puccia R. Polymorphism in the gene coding for the immunodominant antigen gp43 from the pathogenic fungus Paracoccidioides brasiliensis. J Clin Microbiol. 2000;38(11):3960–6.Google Scholar
  18. 18.
    Matute DR, Quesada-Ocampo LM, Rauscher JT, McEwen JG. Evidence for positive selection in putative virulence factors within the Paracoccidioides brasiliensis species complex. PLoS Negl Trop Dis. 2008;172(9):e296.Google Scholar
  19. 19.
    Marques SA. Paracoccidioidomicose: atualização epidemiológica, clínica e terapêutica. An Bras Dermtol. 2003;78:135–50.Google Scholar
  20. 20.
    Valle ACF, Wanke B, Wanke N, et al. Tratamento da paracoccidioidomicose: estudo retrospectivo de 500 casos. II–Avaliação dos resultados terapêuticos com sulfanamídicos, anfotericina B, associação sulfometoxazol/trimetropim, cetoconazol e miconazol. An Bras Dermatol. 1993;68:65–70.Google Scholar
  21. 21.
    Ono MA, Kishima MO, Itano EN, Bracarense APFRL, Camargo ZP. Experimental paracoccidiomycosis in dogs. Med Mycol. 2003;41:265–8.PubMedCrossRefGoogle Scholar
  22. 22.
    Fontana FF, Santos CTB, Esteves FM, Rocha A, Fernandes GF, Amaral CC, Domingues MA, Camargo ZP, Silva-Vergara, ML. Seroepidemiological survey of paracoccidioidomycosis infection among urban and rural dogs from Uberaba, Minas Gerais, Brazil. Mycopathologia. 2009; 169(3): 159–155 (Epub ahead of print).Google Scholar
  23. 23.
    Mós EN, Fava-Netto C. Contribuição ao estudo da paracoccidioidomicose I. Possível papel epidemiológico dos cães. Estudo sorológico e anátomo-patológico. Rev Inst Med Trop São Paulo. 1974;16:154–9.Google Scholar
  24. 24.
    Baumgardener DJ, Paretsky DP, Yopp AC. The epidemiology of blastomycosis in dogs: north central Wisconsin, USA. J Med Vet Mycol. 1995;33(3):171–6.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Marconi Rodrigues de Farias
    • 1
  • Larissa Anuska Zeni Condas
    • 2
  • Márcio Garcia Ribeiro
    • 2
  • Sandra de Moraes Gimenes Bosco
    • 3
    Email author
  • Marisol Dominguez Muro
    • 4
  • Juliana Werner
    • 5
  • Raquel Cordeiro Theodoro
    • 3
  • Eduardo Bagagli
    • 3
  • Sílvio Alencar Marques
    • 6
  • Marcello Franco
    • 7
  1. 1.Department of Clinical Veterinary Medicine for Companion AnimalsPUCPRSão José dos PinhaisBrazil
  2. 2.Department of Veterinary Hygiene and Public HealthFMVZ – UNESP – Univ Estadual PaulistaBotucatuBrazil
  3. 3.Department of Microbiology and ImmunologyIBB – UNESP – Univ Estadual PaulistaBotucatuBrazil
  4. 4.Department of MycologyParana University HospitalCuritibaBrazil
  5. 5.Werner & Werner Pathology LaboratoryCuritibaBrazil
  6. 6.Department of Dermatology and RadiotherapyFMB – UNESP – Univ Estadual PaulistaBotucatuBrazil
  7. 7.Department of PathologyFederal University of São Paulo, UNIFESPSão PauloBrazil

Personalised recommendations