Skip to main content
SpringerLink
Log in

Xanthium italicum, Xanthium strumarium and Arctium lappa as new hosts for Diaporthe helianthi

  • Published:
Mycopathologia Aims and scope Submit manuscript

Abstract

Sunflower (Helianthus annuus) stem canker caused by Diaporthe helianthi is one of the most important sunflower diseases in Croatia. Until recently, sunflower was the only known host for D. helianthi. In our research carried out in the area of Eastern Croatia, isolates of Diaporthe/Phomospis were collected from Xanthium italicum, X. strumarium and Arctium lappa. Using morphological, cultural and molecular ITS rDNA data, isolates from these weeds were identified as D. helianthi. The following isolates were used in the pathogenicity test: one isolate originated from sunflower (Su5/04), three from X. italicum (Xa2, Xa3 and Xa5), two from X. strumarium (Xa9 and Xa12), one from Xanthium sp. (Xa13) and one from A. lappa (Ar3). According to the results, it was determined that isolate Xa5 (originated from X. italicum) was the most pathogenic to sunflower stems. The average length of the lesion was 11.3 cm. The lowest level of pathogenicity was found in Xa9 (isolated from X. strumarium). The length of the lesion was 0.1 cm.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  1. Uecker FA. A world list of Phomopsis names with notes on nomenclature, morphology and biology. Mycol Mem. 1988;13:1–323.

    Google Scholar 

  2. Rehner SA, Uecker FA. Nuclear ribosomal internal transcribed spacer phylogeny and host diversity in the coelomycete Phomopsis. Can J Bot. 1994;72:1666–74.

    Article  CAS  Google Scholar 

  3. Farr FD, Castlebury AL, Pardo-Schultheiss AR. Phomopsis amygdali causes peach shoot blight of cultivated peach trees in the southeastern United States. Mycologia. 1999;91:1008–15.

    Article  Google Scholar 

  4. Murali TS, Suryanarayanan TS, Geeta R. Endophytic Phomopsis species: host range and implications for diversity estimates. Can J Microbiol. 2006;52:673–80.

    Article  CAS  PubMed  Google Scholar 

  5. Van Rensburg JCJ, Lamprecht SC, Groenewald JZ, Castlebury LA, Crous PW. Characterisation of Phomopsis spp. associated with die-back of rooibos (Aspalathus linearis) in South Africa. Stud Mycol. 2006;55:65–74.

    Article  PubMed  Google Scholar 

  6. Mihaljcevic M, Petrov M, Muntanola-Cvetkovic M. Phomopsis sp., a new sunflower parasite in Yugoslavia. Savremena Poljoprivreda. 1980;28:531–9.

    Google Scholar 

  7. Muntanola-Cvetkovic M, Mihaljcevic M, Petrov M. On the identity of the causative agent of a serious Phomopsis—Diaporthe disease in sunflower plants. Nova Hedwigia. 1981;34:417–35.

    Google Scholar 

  8. Homecken M, Franca Neto JB. Phomopsis sp. a new pathogen of sunflower. In: Resultados de Pesquisa de Girassol, Empresa Brasileira de Pesquisa Agropecuaria, Londrina (BR) 1982. p. 665.

  9. Herr LJ, Lipps PE, Watters BL. Diaporthe stem canker of sunflower. Plant Dis. 1983;67:911–3.

    Article  Google Scholar 

  10. Vőrős J, Leranth J, Vajna I. Overwintering of Diaporthe helianthi a new destructive pathogen of sunflower in Hungary. Acta Phytopathol Acad Sci Hung. 1983;18:303–5.

    Google Scholar 

  11. Iliescu H, Jinga V, Ciurea A, Ionita A. Investigations related to the prognosis of sunflower stem canker (Diaporthe helianthi). Helia. 1985;8:51–6.

    Google Scholar 

  12. Regnault Y. Preliminary observations on Phomopsis of sunflower. Bull CETIOM. 1985;92:13–20.

    Google Scholar 

  13. Zazzerini A, Tosi L, Losavio N. Rilievi fitopatologici su varietà di girasole a confronto nel 1987. L’Informatore Agrario. 1988;13:85–8.

    Google Scholar 

  14. Madjidich-Ghassemi. A new sunflower disease in Iran caused by Phomopsis helianthi. Proceedings of the 12th International Sunflower Conference, Novi Sad, Yugoslavia 1988. p. 108–9.

  15. Rauf-Butta A, Rehbar MH, Sayed Irfan A, Iftikhar A. Prevalence and incidence of sunflower diseases in Pakistan. Helia. 1993;16:93–8.

    Google Scholar 

  16. Degener J, Melchinger AE, Hahn V. Interspecific hybrids as source of resistance to Sclerotinia and Phomopsis in sunflower breeding. Helia. 1999;22:49–60.

    Google Scholar 

  17. Demazure B. Phomopsis: Du progres dans les varietés. Grande Cult Infos. 1995;41:39–41.

    Google Scholar 

  18. Franco DA, Morales O. Influence of sunflower stem canker (Diaporthe helianthi) on seed quality and yield during seed development. Helia. 1997;20:57–62.

    Google Scholar 

  19. Laville J. Cahier technique tournesol: maladie. Paris: CETIOM; 1986.

    Google Scholar 

  20. Says-Lesage V, Roeckel-Drevet P, Viguié A, Tourvielle J, Nicolas P, Tourvieille de Labrouhe D. Molecular variability within Diaporthe/Phomopsis helianthi from France. Phytopathology. 2002;92:308–13.

    Article  PubMed  Google Scholar 

  21. Pecchia S, Mercatelli E, Vannacci G. Intraspecific diversity within Diaporthe helianthi: evidence from rDNA intergenic spacer (IGS) sequence analysis. Mycopathologia. 2004;157:317–26.

    Article  CAS  PubMed  Google Scholar 

  22. Rekab D, Del Sorbo G, Reggio C, Zoina A, Firrao G. Polymorphisms in nuclear rDNA and mtDNA reveal the polyphyletic nature of isolates of Phomopsis pathogenic to sunflower and a tight monophyletic clade of defined geographic origin. Mycol Res. 2004;108:393–402.

    Article  CAS  PubMed  Google Scholar 

  23. Vergara M, Cristani C, Regis C, Vannacci G. A coding region in Diaporthe helianthi reveals genetic variability among isolates of different geographic origin. Mycopathologia. 2004;158:123–30.

    Article  CAS  PubMed  Google Scholar 

  24. Dinoor A. Role of wild and cultivated plants in the epidemiology of plant diseases in Israel. Annu Rev Phytopathol. 1974;12:413–36.

    Article  Google Scholar 

  25. Hepperly PR, Kirkpatrick BL, Sinclair JB. Abutilon theophrasti: wild host for three fungal parasites of soybean. Phytopathology. 1980;70:307–10.

    Article  Google Scholar 

  26. Roy KW, Miller WA, McLean KS. Survey of pathogenic genera of fungi on foliage of weeds in Mississippi. Can J Plant Pathol. 1994;16:25–9.

    Google Scholar 

  27. Roy KW, Ratnayake S, McLean K. Colonization of weeds by Phomopsis longicolla. Can J Plant Pathol. 1997;19:193–6.

    Google Scholar 

  28. Mengistu A, Reddy N. Detection of Phomopsis longicolla T. W. Hobbs and its pathogenicity on Glycine max (L.) Merr. and weed hosts. Seed Technol. 2005;27:97–100.

    Google Scholar 

  29. Vrandecic K, Cosic J, Riccioni L, Duvnjak T, Jurkovic D. Isolation of Diaporthe phaseolorum var. caulivora from Abutilon theophrasti in Croatia. Plant Pathol. 2005;54(4):576.

    Article  Google Scholar 

  30. Vrandecic K, Jurkovic D, Cosic J. Effect of Diaporthe/Phomopsis species isolated from soybean and Abutilon theophrasti on soybean seed germination. J Phytopathol. 2006;154:725–8.

    Article  Google Scholar 

  31. Piven’ VT, Dolzhenko EG, Slysar’ eL. Cyclahaena xanthifolia—a potential alternative host of Phomopsis species. Zashchita i Karantin Rastenii. 2000;5:28–9.

    Google Scholar 

  32. Van Niekerk JM, Groenewald JZ, Farr DF, Fourie PH, Halleen F, Crous PW. Reassessment of Phomopsis species on grapevines. Aust Plant Pathol. 2005;34:1–13.

    Article  Google Scholar 

  33. Tranel PJ, Jeschke MR, Wassom JJ, Maxwell DJ, Wax LM. Variation in soybean (Glycine max (L.) Merr.) interference among common cocklebur (Xanthium strumarium L.) accessions. Crop Prot. 2003;22:375–80.

    Article  Google Scholar 

  34. Pál R. Invasive plants threaten Segetal weed vegetation of south Hungary. Weed Technol. 2004;18:1314–8.

    Article  Google Scholar 

  35. Nikandrow A, Weidemann GJ, Auld BA. Incidence and pathogenicity of Colletotrichum orbiculare and a Phomopsis sp. on Xanthium spp. Plant Dis. 1990;74:796–9.

    Article  Google Scholar 

  36. Carriere JB, Petrov M. Diaporthe (Phomopsis) sp., a new pathogen of cocklebur (Xanthium italicum Moretti) and of sunflower (Helianthus annuus L.). Helia. 1990;13:93–106.

    Google Scholar 

  37. Yang SM, Gulya TJ. Groups of Diaporthe/Phomopsis isolates obtained from cultivated sunflower. Phytopathology. 1984;74:7.

    Article  Google Scholar 

  38. Muntanola-Cvetkovic M, Vukojevic J, Mihaljcevic M. Cultural growth patterns and incompatibility reactions in Diaporthe and Phomopis populations. J Phytopathol. 1996;144:285–95.

    Article  Google Scholar 

  39. Jurkovic D, Vrandecic K, Cosic J, Riccioni L, Duvnjak T. Morphological identification of Diaporthe/Phomopsis sp. isolated from Xanthium italicum. Poljoprivreda. 2007;13:10–4.

    Google Scholar 

  40. Wehmeyer LE. The genus Diaporthe Nitschke and its segregates. University of Michigan Studies Sci Ser 9:1933.

  41. Cenis JL. Rapid extraction of fungal DNA for PCR amplification. Nucleic Acids Res. 1992;20:2380.

    Article  CAS  PubMed  Google Scholar 

  42. White TJ, Bruns T, Lee S, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR protocols: a guide to methods and applications. New York: Academic Press; 1990. p. 315–22.

    Google Scholar 

  43. Tamura K, Dudley J, Nei M, Kumar S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol. 2007;24:1596–9.

    Article  CAS  PubMed  Google Scholar 

  44. Nei M, Kumar S. Molecular evolution and phylogenetics. New York: Oxford University Press; 2000.

    Google Scholar 

  45. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution. 1985;39:783–91.

    Article  Google Scholar 

  46. Schneiter AA, Miller JF. Description of sunflower growth stages. Crop Sci. 1981;2:901–3.

    Article  Google Scholar 

  47. Tourvielle D, Vear F, Pelletier C. Use of two mycelium tests in breading sunflower resistant to Phomopsis. Proceedings of 12th Sunflower conference, Novi Sad, Yugoslavia 1988:2: p. 110–4.

  48. Duncan DB. Multiple ranges and multiple F-test. Biometrics. 1955;11:1–42.

    Article  Google Scholar 

  49. SAS/STAT. User’s guide, version 8. Cary: SAS Institute; 1999.

    Google Scholar 

  50. Montuschi C, Collina M, Antoniacci L, Cicognani E, Rimondi S, Trapella R, et al. Preliminary studies on biology and epidemiology of Valsa ceratosperma (Cytospora vitis), the causal agent of bark canker on pear in Italy. IOBC WPRS BULL. 2006;29(1):183–94.

    Google Scholar 

  51. Singh MP, Janso JE, Brady SF. Cytoskyrins and cytosporones produced by Cytospora sp. CR200: taxonomy, fermentation and biological activities. Mar Drugs. 2007;5(3):71–84.

    CAS  PubMed  Google Scholar 

  52. Castlebury LA, Farr DF, Rossman AY, Jaklitsch W. Diaporthe angelicae comb. nov., a modern description and placement of Diaporthopsis in Diaporthe. Mycoscience. 2003;44:203–8.

    Google Scholar 

  53. Santos JM, Phillips AJL. Resolving the complex of Diaporthe (Phomopsis) species occurring on Foeniculum vulgare in Portugal. Fungal Divers. 2009;34:109–23.

    Google Scholar 

  54. Kuleci E, Tunali B, Berner D, Cavin C, Castlebury L. First report of leaf anthracnose caused by Phomopsis convolvuli on field bindweed in Turkey. Plant Dis. 2009;93:847.

    Article  Google Scholar 

  55. Castlebury LA, Rossman AY, Jaklitsch WJ, Vasilyeva LN. A preliminary overview of the Diaporthales based on large subunit nuclear ribosomal DNA sequences. Mycologia. 2002;94:1017–31.

    Article  CAS  Google Scholar 

  56. Muntanola-Cvetkovic M, Mihaljcevic M, Vukojevic J. Dosadašnji rezultati ispitivanja Phomopsis/Diaporthe helianthi na suncokretu, I—mikološki aspekti. Zaštita bilja. 1988;39:469–78.

    Google Scholar 

  57. Jurkovic D, Culek M. Abutilon theophrasti Medik—a new host for Sclerotinia sclerotiorum (Lib.) de Bary in Croatia. Acta Phytopathol et Entomol Hung. 1997;32:307–12.

    Google Scholar 

  58. Viguie A, Masirevic S, Vear F, Grezes-Besset B, Tourvieille de Labrouhe D. Comparison of aggressive isolates of Phomopsis/Diaporthe helianthi (which causes phomopsis in sunflowers) of French and Yugoslav origin. Oléagineux, Corps Gras, Lipides. 1999;6:267–72.

    Google Scholar 

  59. Degener J, Melchinger AE, Hahn V. Resistance in the leaf and stem of sunflower after infection with two isolates of Phomopsis. Plant Breed. 1999;118:405–10.

    Article  Google Scholar 

  60. Mihaljcevic M, Vukojevic J. Diaporthe (Phomopsis) spp. on weeds as possible causative agents of sunflower stem canker in the Vojvodina province. Helia. 1994;17:39–48.

    Google Scholar 

  61. Brayford D. Variation in Phomopsis isolates from Ulmus species in the British Isles and Italy. Mycol Res. 1990;94:691–7.

    Article  Google Scholar 

  62. Janse van Rensburg JC, Lamprecht SC, Groenewald JZ, Castlebury LA, Crous PW. Characterisation of Phomopsis spp. associated with die-back of rooibos (Aspalathus linearis) in South Africa. Stud Mycol. 2006;55:65–74.

    Article  Google Scholar 

Download references

Acknowledgments

The authors would like to thank Mrs. Nicoletta Pucci (CRA-PAV, Rome) for her assistance related to molecular analyses. This work was partially financially supported by the Ministry of Science, Education and Sports of the Republic of Croatia, Project No. 079-0790570-2995.

Author information

Authors and Affiliations

  1. Faculty of Agriculture in Osijek, Trg sv. Trojstva 3, 31000, Osijek, Croatia

    Karolina Vrandecic, Drazenka Jurkovic & Jasenka Cosic

  2. CRA-Centro di ricerca per la patologia vegetale, Via C. G. Bertero 22, 00156, Rome, Italy

    Luca Riccioni

  3. Agriculture Institute Osijek, Juzno predgradje 17, 31000, Osijek, Croatia

    Tomislav Duvnjak

Authors
  1. Karolina Vrandecic
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Drazenka Jurkovic
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Luca Riccioni
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jasenka Cosic
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Tomislav Duvnjak
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Karolina Vrandecic.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Vrandecic, K., Jurkovic, D., Riccioni, L. et al. Xanthium italicum, Xanthium strumarium and Arctium lappa as new hosts for Diaporthe helianthi. Mycopathologia 170, 51–60 (2010). https://doi.org/10.1007/s11046-010-9289-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11046-010-9289-2

Keywords

Search

Navigation