Mycopathologia

, Volume 169, Issue 4, pp 287–295

In Vitro Investigation of Antifungal Activity of Allicin Alone and in Combination with Azoles Against Candida Species

  • Alireza Khodavandi
  • Fahimeh Alizadeh
  • Farzad Aala
  • Zamberi Sekawi
  • Pei Pei Chong
Article

Abstract

Candidiasis is a term describing infections by yeasts from the genus Candida, and the type of infection encompassed by candidiasis ranges from superficial to systemic. Treatment of such infections often requires antifungals such as the azoles, but increased use of these drugs has led to selection of yeasts with increased resistance to these drugs. In this study, we used allicin, an allyl sulfur derivative of garlic, to demonstrate both its intrinsic antifungal activity and its synergy with the azoles, in the treatment of these yeasts in vitro. In this study, the MIC50 and MIC90 of allicin alone against six Candida spp. ranged from 0.05 to 25 μg/ml. However, when allicin was used in combination with fluconazole or ketoconazole, the MICs were decreased in some isolates. Our results demonstrated the existing synergistic effect between allicin and azoles in some of the Candida spp. such as C. albicans, C. glabrata and C. tropicalis, but synergy was not demonstrated in the majority of Candida spp. tested. Nonetheless, In vivo testing needs to be performed to support these findings.

Keywords

Allicin Candida spp. Fluconazole Ketoconazole Antifungal activity 

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Alireza Khodavandi
    • 1
  • Fahimeh Alizadeh
    • 2
  • Farzad Aala
    • 3
  • Zamberi Sekawi
    • 4
  • Pei Pei Chong
    • 1
    • 5
  1. 1.Department of Biomedical Sciences, Faculty of Medicine and Health SciencesUniversiti Putra MalaysiaSerdangMalaysia
  2. 2.Institute of Tropical AgricultureUniversiti Putra MalaysiaSerdangMalaysia
  3. 3.Department of Biology, Faculty of ScienceUniversiti Putra MalaysiaSerdangMalaysia
  4. 4.Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health SciencesUniversiti Putra MalaysiaSerdangMalaysia
  5. 5.Institute of BioscienceUniversiti Putra MalaysiaSerdangMalaysia

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