Mycopathologia

, 168:227

Enhancement of Candida albicans Virulence After Exposition to Cigarette Mainstream Smoke

  • Fernanda Brasil Baboni
  • Dayton Barp
  • Ana Claudia Santos de Azevedo Izidoro
  • Lakshman Perera Samaranayake
  • Edvaldo Antonio Ribeiro Rosa
Article

Abstract

The habit of cigarette smoking is associated with higher oral candidal carriage and possible predisposition to oral candidosis. The effects of exposure to smoke on the virulence properties of oral yeasts remain obscure. Hence, we showed in vitro the effect of cigarette smoke condensate (CSC) on ten clinical isolates of Candida albicans obtained from nonsmoking volunteers, as well the type-strain CBS562. CSC was generated by complete burn of five commercial cigarettes in an in-house smoking machine and used to prepare the culture broth in which the strains were grown. In 24-h intervals (T24, T48, and T72), the cells were harvested, washed, subcultured, and the resultant growth were evaluated for possible variations for secreted aspartyl protease, phospholipase, chondroitinase, and hemolysins, adhesion to acrylic and cell surface hydrophobicity (CSH). The results indicated a temporal increase in the secretion rates of enzymes, particularly when yeast cells were exposed to CSC for 48–72 h (P < 0.05). Similarly, adhesion to acrylic and CSH increased with exposure period (P < 0.05). Based on foregoing, we concluded that CSC may promote significant enhance in the secretion of candidal histolytic enzymes and adherence to denture surfaces, thereby promoting oral yeast carriage and possible infection.

Keywords

Candida albicans Cigarette smoke Virulence 

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Fernanda Brasil Baboni
    • 1
  • Dayton Barp
    • 1
  • Ana Claudia Santos de Azevedo Izidoro
    • 1
    • 2
  • Lakshman Perera Samaranayake
    • 3
  • Edvaldo Antonio Ribeiro Rosa
    • 1
    • 3
  1. 1.Laboratory of Stomatology, CCBSPontifical Catholic University of ParanáCuritibaBrazil
  2. 2.Dental ClinicsGeneral Hospital of CuritibaCuritibaBrazil
  3. 3.Oral Biosciences Unity, Prince Philip Dental HospitalUniversity of Hong KongHong Kong SARChina

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