, Volume 168, Issue 4, pp 203–211 | Cite as

Development of Colletotrichum acutatum on Tolerant and Susceptible Olea europaea L. cultivars: A Microscopic Analysis

  • Sónia GomesEmail author
  • Pilar Prieto
  • Paula Martins-Lopes
  • Teresa Carvalho
  • Antonio Martin
  • Henrique Guedes-Pinto


Colletotrichum acutatum is a cosmopolitan and damaging plant pathogen of temperate, subtropical, and tropical fruits and causes anthracnose on olive (Olea europaea L.). Three olive cultivars showing a variable response to infection by C. acutatum were selected to a preliminary study of pathogen development. Fruit samples, from susceptible and tolerant cultivars, were taken at 0, 24, 48, 72, and 192 h after inoculation for a microscopic and histological study of the infection and colonization process. The aim of this study was to compare the infection process: conidial germination, germ tube and appressorium formation, hyphal growth, and mesocarp colonization in susceptible and tolerant olive cultivars as a condition for further exploration of disease development, which is required to develop cultivars with improved resistance to anthracnose. The rate of mesocarp colonization differed between the susceptible and tolerant cultivars, and both intracellular hemibiotrophy and subcuticular intramural necrotrophy were observed. Hemibiotrophic infection predominated in the moderately tolerant cultivar.


Host–pathogen interactions Infection processes Pathogen development 



This work was supported by Project ‘POCTI/AGR/57817/2004’ and a Ph.D. grant to Sónia Gomes, ‘SFRH/BD/25384/2005’ from the Fundação para a Ciência e Tecnologia, and by a ‘Proyecto de Excelencia- AGR649’ from the Junta de Andalucía.

Supplementary material

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Supplementary material (DOC 3597 kb)


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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Sónia Gomes
    • 1
    Email author
  • Pilar Prieto
    • 2
  • Paula Martins-Lopes
    • 1
  • Teresa Carvalho
    • 3
  • Antonio Martin
    • 2
  • Henrique Guedes-Pinto
    • 1
  1. 1.Institute of Biotechnology and BioengineeringCentre of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro (IBB/CGB-UTAD)Vila RealPortugal
  2. 2.Department of Mejora Genética VegetalInstituto de Agricultura Sostenible (C.S.I.C.)CordobaSpain
  3. 3.National Plant Breeding StationElvasPortugal

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