Mycopathologia

, Volume 168, Issue 2, pp 73–77

Disruption of the GPI Protein-Encoding Gene IFF4 of Candida albicans Results in Decreased Adherence and Virulence

  • Marie Kempf
  • Jane Cottin
  • Patricia Licznar
  • Corinne Lefrançois
  • Raymond Robert
  • Véronique Apaire-Marchais
Article

Abstract

Candida albicans is the most important cause of systemic fungal infection in immunocompromised humans. Candidiasis is often initiated by the adherence and the colonization of inert surfaces such as peripheral venous catheters, central catheters, prosthetic cardiac valves, and other prostheses. We have studied the early stage of adherence and have shown that the disruption of C. albicansIFF4 gene encoding a GPI-anchor protein, led to a decrease of adherence of the germ tubes to plastic. Here, we demonstrated the role of the IFF4 gene in adherence to silicone catheter, as well as in virulence using a murine model of disseminated candidiasis. The iff4 Δ null mutant showed both a decrease of adherence to silicone catheter and a reduction of virulence. This work presents evidence for the importance of the IFF4 gene in host-fungal interaction.

Keywords

Candida albicans IFF4 gene Adherence Catheter Virulence 

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Marie Kempf
    • 1
    • 2
  • Jane Cottin
    • 1
    • 2
  • Patricia Licznar
    • 1
  • Corinne Lefrançois
    • 1
  • Raymond Robert
    • 1
  • Véronique Apaire-Marchais
    • 1
  1. 1.Groupe d’Etude des Interactions Hôte-PathogèneUPRES EA 3142, UFR des Sciences Pharmaceutiques et d’Ingénierie de la SantéAngers CedexFrance
  2. 2.Laboratoire de BactériologieCentre Hospitalier Universitaire d’AngersAngers Cedex 9France

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