Isoelectric focusing and ELISA evaluation of a Blastomyces dermatitidis human isolate
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Blastomyces dermatitidis is a dimorphic fungal organism and the causative agent of blastomycosis. This organism is endemic east of the Mississippi river as is the fungal organism Histoplasma capsulatum. This study was performed to determine if sensitive and specific antigens from the B. dermatitidis yeast phase lysate (human isolate 592) could be separated using isoelectric focusing (IEF) to eliminate antigens that are cross-reactive with H. capsulatum. Indirect enzyme linked immunosorbent assays were performed to test for reactivity and cross-reactivity and indicate that certain fractions (4–6) were highly reactive. Fraction 16 exhibited a high degree of cross-reactivity with H. capsulatum. This study indicates that IEF may be a useful method for the separation of B. dermatitidis proteins.
KeywordsBlastomycosis Cross-reactivity ELISA Isoelectric focusing Protein
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We would like to thank Dr. A.F. DiSalvo for providing the B. dermatitidis isolate 592. This research was funded in part by a grant from the Department of Biological Sciences, and from the Graduate Student Research and Scholarship Committee (S05-111), Idaho State University, Pocatello, Idaho.
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