Mycopathologia

, 163:21

Ochratoxin A and citrinin nephrotoxicity in New Zealand White rabbits: an ultrastructural assessment

  • Manoj Kumar
  • Prabhaker Dwivedi
  • Anil K. Sharma
  • Nittin Dev Singh
  • Rajendra D. Patil
Article

DOI: 10.1007/s11046-006-0079-9

Cite this article as:
Kumar, M., Dwivedi, P., Sharma, A.K. et al. Mycopathologia (2007) 163: 21. doi:10.1007/s11046-006-0079-9

Abstract

In the present investigation, ochratoxin A (OTA) (0.75 mg/kg feed) and citrinin (CIT) (15 mg/kg feed) were fed alone and in combination to young growing New Zealand White rabbits for 60 days to evaluate renal ultrastructural alterations. The severity and intensity of renal ultrastructural changes varied with the type of the treatment, and predominant and consistent lesions were recorded in the proximal convoluted tubule (PCT) lining cells. The significant changes in mitochondria, the most affected cell organelle in all the treatment groups, included mitochondrial disintegration and distortion, pleomorphism, cluster formation and misshapen appearance such as signet ring, dumbbell, cup and U shapes. Intra-cisternal sequestrations of involuting mitochondria, and thickening of basal layer of PCT epithelial cells with partial detachment, were the characteristic features observed in OTA and combination treatments. CIT treatment revealed crenated nucleus, loss of nucleolus, depletion of cytoplasmic organelles, mitochondrial pleomorphism, nuclear fragmentation, uniform folding of cell membrane and cytoplasmic vacuolations in the PCTs. Focal thickening of the glomerular basement membrane and degeneration of endothelial cells were the prominent alterations in the glomeruli in OTA and combination treatments. Distal convoluted tubules were unaffected in CIT treatment, however, mild to moderate lesions were observed in OTA and combination treated rabbits. It may be concluded that on simultaneous exposure, CIT potentiated the toxic effects of OTA on renal ultrastructure.

Keywords

citrinin mitochondria ochratoxin A proximal convoluted tubule rabbits ultrastructural changes 

Abbreviations

BM

basement membrane

CIT

citrinin

DCT

distal convoluted tubule

FP

foot processes of podocytes

ICS

intra-cisternal sequestration

IS

interstitial space

Lu

lumen

M

mitochondria

Mv

microvilli

N

nucleus

OTA

ochratoxin A

PCT

proximal convoluted tubule

RER

rough endoplasmic reticulum

US

urinary space

Copyright information

© Springer 2007

Authors and Affiliations

  • Manoj Kumar
    • 1
  • Prabhaker Dwivedi
    • 1
  • Anil K. Sharma
    • 1
  • Nittin Dev Singh
    • 1
  • Rajendra D. Patil
    • 1
  1. 1.Mycotic and Mycotoxic Disease Laboratory, Division of PathologyIndian Veterinary Research InstituteIzatnagar, BareillyIndia

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