, 162:369 | Cite as

Isolation of Paecilomyces lilacinus (Thom) Samson (Ascomycota: Hypocreales) from the Chagas disease vector, Triatoma infestans Klug (Hemiptera: Reduviidae) in an endemic area in Argentina

  • Gerardo A. Marti
  • Claudia C. López Lastra
  • Sebastian A. Pelizza
  • Juan J. García


A survey for entomopathogenic fungi of the Chagas disease vector Triatoma infestans was conducted in two provinces of Argentina from March–December 2003. Field-collected insects that died in the laboratory were individually maintained in moist chamber and incubated at 22 °C. Triatominae adults infected with the fungus Paecilomyces lilacinus were found at El Quebracho (27°34′S–64°31′W), Santiago del Estero province, Argentina, in December 2003. Paecilomyces lilacinus was cultured and isolated from infected insects in SDAY, PYG and MEA media. Pathogenicity tests were conducted and positive results were recorded. The median survival time (MST) of T. infestans exposed to a P. lilacinus conidial suspension was 12.8 days, and 100% mortality occurred at 30 days post-treatment. This is the first record of natural infection caused by P. lilacinus in T. infestans in the world.


entomopathogenic fungi Paecilomyces lilacinus Triatominae Triatoma infestan


  1. 1.
    Segura, EL, Cura, EN, Sosa Estan, SA, Andrade, J, Lansetti, JC, Rissio, AM, Campanini, A, Blanco, SB, Gurtler, RE, Alvarez, M 2000Long-term effects of a nationwide control program on the seropositivity for Trypanosoma cruzi infection in young men from ArgentinaAm J Trop Med Hyg62353362PubMedGoogle Scholar
  2. 2.
    Samson, RA, Evans, HC 1974Notes on entomogenous fungi f rom Ghana II. The genus Akanthomyces Acta Bot Neerl232835Google Scholar
  3. 3.
    Moncayo, A 1997Chagas’ disease: epidemiology and prospects for interruption of transmission in the AmericasWorld Health Stat Q45276279Google Scholar
  4. 4.
    Cerisola, JA, Prado, CE, Rohwedder, R, Bozzini, JP 1971 Blastocrithidia triatomae (n. sp.) found in Triatoma infestans from ArgentinaJ Protozool18503506PubMedGoogle Scholar
  5. 5.
    Muscio, OA, Torre, JL, Scodeller, EA 1987Small non-occluded viruses from triatomine bug Triatoma infestans (Hemiptera: Reduviidae)J Invertebr Pathol49218220CrossRefPubMedGoogle Scholar
  6. 6.
    Marti, GA, Scorsetti, AC, Siri, A, López Lastra, CC 2005Isolation of Beauveria bassiana (Bals.) Buill. (Deuteromycotina: Hy phomycetes) from the Chagas diseases vector Triatoma infestans (Hemiptera: Reduv iidae) in ArgentinaMycopathologia159389391CrossRefPubMedGoogle Scholar
  7. 7.
    Lent, H, Wygodzinsky, P 1979Revision of the Triatominae (Hemiptera, Reduviidae), and their significance as vectors of Chagas´ DiseasesBull Am Mus Nat Hist163123520Google Scholar
  8. 8.
    Lacey, LA 1997:Manual of techniques in insect pathologyAcademic PressLondon409Google Scholar
  9. 9.
    Humber, RA 1997Fungi: IdentificationLacey, L eds. Manual of Techniques in Insect PathologyAcademic PressLondon53185Google Scholar
  10. 10.
    Samson, RA 1974Paecilomyces and some allied hyphomycetesStud Mycol61119Google Scholar
  11. 11.
    Lane, BS, Humphreys, AM, Thompson, K, Trinci, APJ 1988ATP Content of stored spores of Paecilomyces farinosus and the use of ATP as criterion of spore viabilityTrans Brit Mycol Soc90109111CrossRefGoogle Scholar
  12. 12.
    Lecuona RE, Díaz BM. Susceptibilidad de Spodoptera frugiperda (J. E. Smith) a los hongos entomopatógenos Nomuraea rileyi, Metarhizium anisopliae y Beauveria bassiana. RIA 30 2001; (1): 25-2.Google Scholar
  13. 13.
    ViStat Program. Boyce Thompson Institute, Ithaca, New York, 1990Google Scholar
  14. 14.
    Parameswaran G, Sankaran T. Record of Beauveria bassiana (Bals.) Vuill. on Linshcosteus sp. (Hemiptera, Reduviidae, Triatominae) in India. J Entom Res 1977; (1): 113-14.Google Scholar
  15. 15.
    Pineda Gutiérrez, F, Saldarriaga Osorio, Y, Calle Osorno, J, Uribe Soto, S 2003Susceptibility of Rhodnius pallescens (Hemiptera: Reduviidae) of fifth instar nymph to the action of Beauveria sppEntomotropica18163168Google Scholar
  16. 16.
    Luz, C, Rocha, LFN, Humber, RA 2003Record of Evlachovaea sp (Hyphomycetes) on Triatoma sordida in the state of Goiás, Brazil, and its activity against Triatoma infestans (Reduviidae, Triatominae)J Med Entomol40451454CrossRefPubMedGoogle Scholar
  17. 17.
    Moraes, AML, Figueiredo, AR, Junqueira, ACV, Costa, GL, Aguiar, RK, Oliveira, PC 2001Fungal flora of the digestive tract of Panstrongylus megistus (Reduviidae) used for experimental xenodiagnosis of Trypanosoma (Schizotripanum) cruzi Chagas, 1909Rev Iberoam Micol187982Google Scholar
  18. 18.
    Moraes, AML, Junqueira, ACV, Costa, GL, Celano, V, Oliveira, PC, Coura, JR 2000Fungal flora of the digestive tract of 5 species of triatomines vectors of Trypanosoma cruzi, Chagas 1909Mycopathologia1514148CrossRefGoogle Scholar
  19. 19.
    Humber, RA 1998Collection of Entomopathogenic Fungal Cultures: Catalog of StrainsUSDA-ARSIthaca, NY, USASupplement to the 1992 catalogGoogle Scholar
  20. 20.
    Spegazzini, C 1911Mycetes ArgentinensesAn Mus Nac Hist Nat B Aires20329467Google Scholar
  21. 21.
    Sherlock, IA, Guitton, N 1982Observaçoes sobre a açao do fungo Metarhizium anisopliae Metschnikoff sobre algumas espécias de Triatominae (Hemiptera, Reduviidae)Revista do Instituto de Medicina Tropical, Sao Paulo24234239Google Scholar
  22. 22.
    Lecuona, RE, Edelstein, JD, Berreta, MF, Rossa, FR, Arcas, JA 2001Evaluation of Beauveria bassiana (Hyphomycetes) strains as potential agents for control of Triatoma infestans (Hemiptera: Reduviidae)J Med Entomol38172179CrossRefPubMedGoogle Scholar
  23. 23.
    Luz, C, Silva, IG, Cordeiro, CMT, Tigano, MS 1998 Beauveria bassiana (Hyphomycetes) as a possible agent for biological control of Chagas disease vectorsJ Med Entomol35977979PubMedGoogle Scholar
  24. 24.
    Sanmartino,  2000Conocimientos sobre la enfermedad de Chagas y factores de riesgo en comunidades epidemiológicamente diferentes de ArgentinaRev Panam Salud Publica/Pan Am J Public Health7173178Google Scholar

Copyright information

© Springer 2006

Authors and Affiliations

  • Gerardo A. Marti
    • 1
  • Claudia C. López Lastra
    • 2
  • Sebastian A. Pelizza
    • 2
  • Juan J. García
    • 2
  1. 1.Centro Regional de Investigaciones Científicas y Transferencias Tecnológicas (CRILAR) (CONICET)La RiojaArgentina
  2. 2.Centro de Estudios Parasitológicos y de Vectores (CEPAVE) (CONICET-UNLP)La PlataArgentina

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