, Volume 162, Issue 3, pp 215–223 | Cite as

Ochratoxins: A global perspective

  • Paul BaymanEmail author
  • James L. Baker


Ochratoxins have been overshadowed by better-known mycotoxins, but they are gaining importance. Here we consider ochratoxins in the context of aflatoxins, which are better understood than ochratoxins on many levels. We review recent work on taxonomic distribution, contamination of commodities, biosynthesis, toxicity and regulatory aspects of ochratoxins. We focus on ochratoxins in coffee, since coffee is becoming a key commodity in ochratoxin research and regulation.


Aspergillus coffee food safety mycotoxin ochratoxin Penicillium 


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  1. 1.
    Størmer, FC 1992Ochratoxin A – a mycotoxin of concernBhatnagar, DLillehoj, EBArora, DK eds. Handbook of Applied Mycology, Vol. 5: Mycotoxins in Ecological SystemsMarcel Dekker, Inc New York403432Google Scholar
  2. 2.
    Hooper J. Polluted pasta causes toxin alarm in Italy. Guardian Unlimited 1/12/2006.,1684524,00.html
  3. 3.
    Matossian, MK 1984Mold poisoning and population growth in England and France, 1750–1850J Econ Hist44669686PubMedCrossRefGoogle Scholar
  4. 4.
    Di Paolo, N, Guarnieri, A, Loi, F, Sacchi, G, Mangiarotti, AM, Di Paolo, M 1993Acute renal failure from inhalation of mycotoxinsNephron64621625PubMedCrossRefGoogle Scholar
  5. 5.
    Merwe, KJ, Steyn, PS, Fourie, L 1965Ochratoxin A, a toxic metabolite produced by Aspergillus ochraceus WilhNature20511121113PubMedCrossRefGoogle Scholar
  6. 6.
    Bennett, JW, Klich, M 2003MycotoxinsClin Microbiol Rev16497516PubMedCrossRefGoogle Scholar
  7. 7.
    Lai, M, Semeniuk, G, Hesseltine, CW 1970Conditions for production of ochratoxin A by Aspergillus species in synthetic mediumAppl Microbiol19542544PubMedGoogle Scholar
  8. 8.
    Ciegler, A, Fennell, DI, Sansing, GA, Detroy, RW, Bennett, GA 1973Mycotoxin-producing strains of Penicillium viridicatum: Classification into subgroupsAppl Microbiol26271278PubMedGoogle Scholar
  9. 9.
    Hesseltine, CW, Vandegraft, EE, Fennell, DI, Smith, ML, Shotwell, OL 1972Aspergilli as ochratoxin producersMycologia64539550PubMedGoogle Scholar
  10. 10.
    Tsubouchi, H, Terada, H, Yamamoto, K, Hisada, K, Sakabe, Y 1995Caffeine degradation and increased ochratoxin production by toxigenic strains of Aspergillus ochraceus isolated from green coffee beansMycopathologia90181186CrossRefGoogle Scholar
  11. 11.
    Varga, J, Kevei, É, Rinyu, E, Téren, J, Kozakiewicz, Z 1996Ochratoxin production by Aspergillus speciesAppl Environ Microbiol6244614464PubMedGoogle Scholar
  12. 12.
    Frisvad, JC, Samson, RA 2000Neopetromyces gen. nov. and an overview of teleomorphs of Aspergillus subg. CircumdatiStud Mycol45201207Google Scholar
  13. 13.
    Baker JL, Bayman P, Mahoney NE, Klich MA, Palumbo JD, Campbell BC. Ochratoxigenic Aspergillus lanosus and A. alliaceus isolates from California tree nut orchards. Proceedings of the 3rd Fungal Genomics, 4th Fumonisin, and 16th Aflatoxin Elimination Workshops, Savannah, Georgia, 2003 [Poster]Google Scholar
  14. 14.
    Peterson, SW 2000Phylogenetic relationships in Aspergillus based on rDNA sequence analysisSamson, RAPitt, JI eds. Classification of Penicillium, Aspergillus: Integration of Modern Taxonomic MethodsHarwood Publishers Reading UK323356Google Scholar
  15. 15.
    Varga, J, Kevei, É, Tóth, B, Kozakiewicz, Z, Hoekstra, RF 2000Molecular analysis of variability within the toxigenic Aspergillus ochraceus speciesCan J Microbiol46593599PubMedCrossRefGoogle Scholar
  16. 16.
    Varga, J, Tóth, B, Rigó, K, Téren, J, Hoekstra, RF, Kozakiewicz, Z 2000Phylogenetic analysis of Aspergillus section Circumdati based on sequences of the internal transcribed spacer regions and the 5.8S rRNA geneFungal Genet Biol307180PubMedCrossRefGoogle Scholar
  17. 17.
    Frisvad, JC, Frank, JM, Houbraken, J, Kuijpers, AFA 2004New ochratoxin producing species of Aspergillus section CircumdatiStud Mycol502343Google Scholar
  18. 18.
    Abarca, ML, Bragulat, MR, Sastellá, G, Cabañes, FJ 1994Ochratoxin A production by strains of Aspergillus niger var. niger Appl Environ Microbiol6026502652PubMedGoogle Scholar
  19. 19.
    Téren, J, Varga, J, Hamari, Z, Rinyu, E, Kevei, F 1996Immunochemical detection of ochratoxin A in black Aspergillus strainsMycopathologia134171176PubMedCrossRefGoogle Scholar
  20. 20.
    Samson, RA, Houbraken, JAMP, Kuijpers, AFA, Frank, JM, Frisvad, JC 2004New ochratoxin A or sclerotium producing species in Aspergillus section Nigri Stud Mycol504561Google Scholar
  21. 21.
    Schuster, E, Dunn-Coleman, N, Frisvad, J, Dijck, P 2002On the safety of Aspergillus niger – a reviewAppl Microbiol Biotechnol59426435PubMedCrossRefGoogle Scholar
  22. 22.
    Blank, G,  Nwoko, U,  Frohlich, A,  Marquardt, R 1998Ochratoxin A production in relation to the growth morphology of Aspergillus alutaceus Lebensm Wiss Technol31210214CrossRefGoogle Scholar
  23. 23.
    Klich MA. Identification of Common Aspergillus Species. Utrecht, Netherlands: Centraalbureau voor Schimmelcultures, 2002Google Scholar
  24. 24.
    Abarca, ML, Accensi, F, Bragulat, MR, Cabañes, FJ 2001Current importance of ochratoxin A-producing Aspergillus sppJ Food Protect64903906Google Scholar
  25. 25.
    Pitt, JI 1987Penicillium viridicatum, Penicillium verrucosum, and production of ochratoxin AAppl Environ Microbiol53266269PubMedGoogle Scholar
  26. 26.
    Land, CJ, Hult, K 1987Mycotoxin production by some wood-associated Penicillium sppLett Appl Microbiol44144Google Scholar
  27. 27.
    Mills, JT, Abramson, D 1982Ochratoxigenic potential of Penicillium spp. isolated from stored rapeseed and cereals in western CanadaCan J Plant Pathol43741CrossRefGoogle Scholar
  28. 28.
    Frisvad, JC, Lund, F, Elmholt, S 2005Ochratoxin A producing Penicillium verrucosum isolates from cereals reveal large AFLP fingerprinting variabilityJ Appl Microbiol98684692PubMedCrossRefGoogle Scholar
  29. 29.
    Skaug, MA, Helland, I, Solvoll, K, Saugstad, OD 2001Presence of ochratoxin A in human milk in relation to dietary intakeFood Addit Contam18321327PubMedGoogle Scholar
  30. 30.
    Paster, N, Lisker, N, Chet, I 1985Accumulation of ochratoxin A in sclerotia of Aspergillus ochraceus Can J Bot63661662CrossRefGoogle Scholar
  31. 31.
    Bucheli, P, Kanchanomai, C, Meyer, I, Pittet, A 2000Development of ochratoxin A during robusta (Coffea canephora) coffee cherry dryingJ Agric Food Chem4813581362PubMedCrossRefGoogle Scholar
  32. 32.
    Vega, FE, Mercadier, G 1998Insects, coffee and ochratoxin AFla Entomol81543544Google Scholar
  33. 33.
    Shotwell, LL, Hesseltine, CW, Goulden, ML., Ochratoxin, A 1969occurrence as natural contaminant of a corn sampleAppl Microbiol17765766PubMedGoogle Scholar
  34. 34.
    Zimmerli, B, Dick, R 1996Ochratoxin A in table wine and grape-juice: Occurrence and risk assessmentFood Addit Contam13655668PubMedGoogle Scholar
  35. 35.
    Pitt, JI 2000Toxigenic fungi: Which are important?Med Mycol38S1722Google Scholar
  36. 36.
    Aziz, NH, Youssef, YA, El-Fouly, MZ, Moussa, LA 1998Contamination of some common medicinal plant samples and spices by fungi and their mycotoxinsBot Bull Acad Sinica (Taiwan)39279285Google Scholar
  37. 37.
    Jorgensen, K 1998Survey of pork, poultry, coffee, beer and pulses for ochratoxinFood Addit Contam15550554PubMedGoogle Scholar
  38. 38.
    Kuiper-Goodman, T, Scott, PM 1989Risk assessment of the mycotoxin ochratoxin ABiomed Environ Sci2179248PubMedGoogle Scholar
  39. 39.
    Olsen M, Jonsson N, Magan N, Banks J, Fanelli C, Rizzo A, Haikara A, Dobson A, Frisvad J, Holmes S, Pajunen E, Persson SJ, Jonsson A. Prevention of Ochratoxin A in Cereals
  40. 40.
    Campbell, BC, Molyneux, RJ, Schatzki, TF 2003Current research on reducing pre- and post-harvest aflatoxin contamination of U.S. tree nutsJ Toxicol Toxin Rev22225266CrossRefGoogle Scholar
  41. 41.
    Bayman, P, Baker, JL, Doster, MA, Michailides, TJ, Mahoney, NE 2002Ochratoxin production by the Aspergillus ochraceus group and A. alliaceus Appl Environ Microbiol6823262329PubMedCrossRefGoogle Scholar
  42. 42.
    Walker R. Mycotoxins of growing interest. Third Joint FAO/WHO/UNEP International Conference on Mycotoxins, Tunis, 1999. food/pdf/ mycotoxins_report_en.pdf
  43. 43.
    Levi, CP, Trenk, HL, Mohr, HK 1974Study of the occurrence of ochratoxin A in green coffee beansJ Assoc Off Anal Chem57866870PubMedGoogle Scholar
  44. 44.
    Romani, S, Sacchetti, G, Chaves López, C, Pinnavaia, GG, Dalla Rosa, M 2000Screening on the occurrence of ochratoxin A in green coffee beans of different origins and typesJ Agric Food Chem4836163619PubMedCrossRefGoogle Scholar
  45. 45.
    Urbano, GR, Taniwaki, MH, Leitão, MFF, Vicentini, MC 2001Occurrence of ochratoxin A-producing fungi in raw Brazilian coffeeJ Food Prot6412261230PubMedGoogle Scholar
  46. 46.
    Blanc, M, Pittet, A, Munoz-Box, R, Viani, R 1998Behavior of ochratoxin A during green coffee roasting and soluble coffee manufactureJ Ag Food Chem46673675CrossRefGoogle Scholar
  47. 47.
    International Coffee Organization (2005). OTA risk management: guidelines for green coffee buying.
  48. 48.
    Patel, S, Hazel, CM, Winterton, AGM, Gleadle, AE 1997Survey of ochratoxin A in UK retail coffeesFood Addit Contam14217222PubMedGoogle Scholar
  49. 49.
    Harris, JP, Mantle, PG 2001Biosynthesis of ochratoxins by Aspergillus ochraceus Phytochemistry58709716PubMedCrossRefGoogle Scholar
  50. 50.
    Lee SE, Bayman P, Baker JL, Campbell BC. Inhibition of ochratoxin biosynthesis by naturally occurring alkaloids. [in preparation]Google Scholar
  51. 51.
    O’Callaghan, J, Caddick, MX, Dobson, ADW 2003A polyketide synthase gene required for ochratoxin A biosynthesis in Aspergillus ochraceus Microbiology14934853491PubMedCrossRefGoogle Scholar
  52. 52.
    Yu, J, Chang, P, Ehrlich, K, Cary, JW, Bhatnagar, D, Cleveland, TE, Payne, GA, Linz, JE, Woloshuk, CP, Bennett, JW 2004The clustered pathway genes in aflatoxin biosynthesisAppl Environ Microbiol7012531262PubMedCrossRefGoogle Scholar
  53. 53.
    O’Brien, E, Dietrich, DR 2005Ochratoxin A: The continuing enigmaCrit Rev Toxicol353360PubMedCrossRefGoogle Scholar
  54. 54.
    Bennett, JW 1995From molecular genetics and secondary metabolism to molecular metabolites and secondary geneticsCan J Bot73S917S924Google Scholar
  55. 55.
    Dowd, PF 1989Toxicity of naturally occurring levels of the Penicillium mycotoxins citrinin, ochratoxin A, and penicillic acid to the corn earworm, Heliothis zea, and the fall army worm, Spodoptera frugiperda (Lepidoptera: Noctuidae)Environ Entomol182429Google Scholar
  56. 56.
    Breitholtz-Emanuelsson, A, Olsen, M, Oskarsson, A, Palminger, I, Hult, K 1993Ochratoxin A in cow’s milk and in human milk with corresponding human blood samplesJ Assoc Off Anal Chem76842846Google Scholar
  57. 57.
    Robens, J, Cardwell, K 2003The costs of mycotoxin management to the USA: Management of aflatoxins in the United StatesJ Toxicol Toxin Rev22139152CrossRefGoogle Scholar
  58. 58.
    Simon, P 1996Ochratoxin and kidney disease in the humanJ Toxicol Tox Rev15239249Google Scholar
  59. 59.
    Codex Alimentarius Commission. Position paper on ochratoxin A. Food and Agriculture Organization of the United Nations, Rome, Italy, 1998. Chemicalcontaminants /ochratoxinpp99_14.pdf
  60. 60.
    Hsieh, MF, Chiu, HY, Lin-Tan, DT, Lin, JL 2004Does human ochratoxin A aggravate proteinuria in patients with chronic renal disease?Ren Fail26311316PubMedCrossRefGoogle Scholar
  61. 61.
    Navas, SA, Sabino, M, Rodriguez-Amaya, DB 2005Aflatoxin M1 and ochratoxin A in a human milk bank in the city of São Paulo, BrazilFood Addit Contam22457462PubMedCrossRefGoogle Scholar
  62. 62.
    Henry, SH, Bosch, FX, Troxell, TC, Bolger, PM 1999Reducing liver cancer–global control of aflatoxinScience28624532454PubMedCrossRefGoogle Scholar
  63. 63.
    Creppy, EE, Baudrimont, I, Belmadani, A, Betbeder, AM 1996Aspartame as a preventive agent of chronic toxic effects of ochratoxin A in experimental animalsJ Toxicol Toxin Rev15207221Google Scholar
  64. 64.
    Schwartz, GG 2002Hypothesis: Does ochratoxin A cause testicular cancer?Cancer Causes Control1391100PubMedCrossRefGoogle Scholar
  65. 65.
    FAO. Worldwide regulations for mycotoxins in food and feed in 2003. 2004.
  66. 66.
    FAO. Reducing ochratoxin A in coffee. 2005. http://www.
  67. 67.
    Ehrlich, KC, Cotty, PJ 2004An isolate of Aspergillus flavus used to reduce aflatoxin contamination in cottonseed has a defective polyketide synthase geneAppl Microbiol Biotechnol65473478PubMedCrossRefGoogle Scholar
  68. 68.
    King, H, Aubert, RE, Herman, WH 1998Global burden of diabetes, 1995–2025Diabetes Care2114141431PubMedGoogle Scholar
  69. 69.
    Cardwell KF, Desjardins A, Henry SH, Munkvold G, Robens J. Mycotoxins: the cost of achieving food security and food quality. APSnet Feature Story, 2001.

Copyright information

© Springer 2006

Authors and Affiliations

  1. 1.Depto. de BiologíaUniversidad de Puerto Rico-Río PiedrasSan JuanUSA
  2. 2.USDA ARS Western Regional Research CenterAlbanyUSA

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