Mycopathologia

, 162:45

Species Distribution and Antifungal Susceptibility Profile of Oral Candida Isolates from HIV-infected Patients in the Antiretroviral Therapy Era

  • Carolina Rodrigues Costa
  • Janine Aquino de Lemos
  • Xisto Sena Passos
  • Crystiane Rodrigues de Araújo
  • Ana Joaquina Cohen
  • Lúcia Kioko Hasimoto e Souza
  • Maria do Rosário Rodrigues Silva
Article

Abstract

In this study, we investigated the yeasts colonization of genus Candida, including C. dubliniensis, isolated of HIV-infected patients oral cavities and we accessed in vitro susceptibility pattern of the Candida isolates to four antifungal agents. Out of 99 patients investigated, 62 (62.6%) were colonized with yeasts. C. albicans was the prevailing species (50%). C. dubliniensis isolates were not recovered in our study. We verified that 8.1% of the yeasts isolated were resistant to fluconazole, 8.1% to itraconazole and 3.2% to voriconazole. The isolates demonstrated very low voriconazole MICs, in which 79% (49/62) presented values of 0.015 μg/ml. All Candida isolates were susceptible to amphotericin B. The results reported here showed that although C. albicans continues to be present in one-half of oral Candida carriage of HIV-infected patients, Candida non-albicans species are increasing among these patients. Besides, the findings of resistant isolates endorse the role of antifungal susceptibility testing whenever antifungal treatment with azoles is planned.

Keywords

antifungal susceptibility asymptomatic oropharyngeal carriage Candida HIV-infected patients 

Abbreviations

DMSO

Dimetyl sulfoxide

HDT

Hospital de Doenças Tropicais

HIV

Human Immunodeficiency Virus

MICs

Minimal Inhibitory Concentrations

MOPS

Morpholinepropanesulfonic acid

NCCLS

National Committee for Clinical Laboratory Standards

OPC

Oropharyngeal candidiasis

SDA

Sabouraud Dextrose Agar

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Copyright information

© Springer 2006

Authors and Affiliations

  • Carolina Rodrigues Costa
    • 1
  • Janine Aquino de Lemos
    • 1
  • Xisto Sena Passos
    • 1
  • Crystiane Rodrigues de Araújo
    • 1
  • Ana Joaquina Cohen
    • 2
  • Lúcia Kioko Hasimoto e Souza
    • 1
  • Maria do Rosário Rodrigues Silva
    • 1
    • 3
  1. 1.Instituto de Patologia Tropical e Saúde Pública daUniversidade Federal de GoiásGoiásBrazil
  2. 2.Hospital de Doenças Tropicais de GoiásGoiásBrazil
  3. 3.Maria do Rosário Rodrigues SilvaGoianiaBrasil

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