, Volume 162, Issue 1, pp 33–37

Detection of IgG and IgM in Sera from Canines with Blastomycosis using Eight Blastomyces dermatitidis Yeast Phase Lysate Antigens



The objective of this study was to compare the efficacy of eight Blastomyces dermatitidis yeast phase lysate antigens (T-58: dog, Tennessee; T-27: polar bear, Tennessee; ERC-2: dog, Wisconsin; B5894: human, Minnesota; SOIL: soil, Canada; B5896: human, Minnesota; 48089: human, Zaire; 48938: bat, India) in the detection of the immunoglobulins IgG and IgM in serum specimens from canines with blastomycosis. An indirect enzyme-linked immunosorbent assay (ELISA, peroxidase system) was used to analyze sera collected during four different intervals post-infection. The yeast lysate antigen 48938 was a reactive antigen for the detection of both IgG (mean absorbance value range: 1.198–2.934) and IgM (mean absorbance value range: 0.505–0.845). For the same sera, antigen T-27 was also effective in the detection of IgG (mean absorbance value range: 0.904–3.356) and antigen 48089 was useful for the detection of IgM (mean absorbance value range: 0.377–0.554). The yeast lysate antigen B5894 proved to be a poor antigen for the detection of both IgG and IgM (mean absorbance value ranges: 0.310–0.744 for IgG, 0.025–0.069 for IgM). Inherent variations in yeast lysate antigens such as these may be utilized to develop improved immunoassay procedures for the specific detection of IgG or IgM in cases of blastomycosis.


Blastomyces dermatitidis ELISA IgG/IgM immunodiagnosis pathogenic fungi 


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  1. 1.
    Bradsher, R 1992BlastomycosisClin Infect Dis14S82S90PubMedGoogle Scholar
  2. 2.
    Murphy, P 1989BlastomycosisJ Am Med26131593161CrossRefGoogle Scholar
  3. 3.
    Causey, WA, Campbell, GD 1992

    Clinical aspects of blastomycosis

    Al-Doory, YDiSalvo, AF eds. BlastomycosisPlenum Medical Book CoNew York165188
    Google Scholar
  4. 4.
    Axtell, RC, Scalarone, GM 2002Serological differences in three Blastomyces dermatitidis strainsMycoses45437442PubMedGoogle Scholar
  5. 5.
    Johnson, SM, Scalarone, GM 1988Preparation and ELISA evaluation of Blastomyces dermatitidis yeast phase lysate antigensDiagn Microbiol Infect Dis118186PubMedCrossRefGoogle Scholar
  6. 6.
    Turner, S, Kaufman, L 1986Immunodiagnosis of blastomycosisSemin Respir Infec12228Google Scholar
  7. 7.
    Wakamoto, A, Fryer, BM, Fisher, MA,  et al. 1997Detection of antibodies and delayed dermal hypersensitivity with different lots of Blastomyces dermatitidis yeast lysate antigen: stability and specificity evaluationsMycoses40303308PubMedCrossRefGoogle Scholar
  8. 8.
    Seawell, BW, Legendre, AM, Scalarone, GM 1990Enzyme immunoassay detection of antibodies in canine blastomycosis using Blastomyces dermatitidis lysate antigensMycoses33483489PubMedGoogle Scholar
  9. 9.
    Axtell, RC, Scalarone, GM 2001Serological differences in two Blastomyces dermatitidis isolates from different geographical regions of North AmericaMycopathologia153141144CrossRefGoogle Scholar
  10. 10.
    Chester, EM, Axtell, RC, Scalarone, GM 2003Blastomyces dermatitidis lysate antigens: antibody detection in serial serum specimens from dogs with blastomycosisMycopathologia156289294PubMedCrossRefGoogle Scholar
  11. 11.
    Green, JH, Harrell, WK, Johnson, JE,  et al. 1980Isolation of an antigen from Blastomyces dermatitidis that is specific for the diagnosis of blastomycosisCurr Microbiol4293296Google Scholar
  12. 12.
    Klein, BJ, Kuritsky, JN, Chappel, WA,  et al. 1986Comparison of the enzyme immunoassay, immunodiffusion, and complement fixation tests in detecting antibody in human sera to the A antigen of Blastomyces dermatitidisAm Rev Respir Dis133144148PubMedGoogle Scholar
  13. 13.
    Turner, SH, Kaufman, L, Jalbert, M 1986Diagnostic assessment of an enzyme-linked immunosorbent assay for human and canine blastomycosisJ Clin Microbiol23294297PubMedGoogle Scholar
  14. 14.
    Klein, BS, Vergeront, JM, Kaufman, L,  et al. 1987Serological tests for blastomycosis: assessments during a large point-source outbreak in WisconsinJ Infect Dis155262268PubMedGoogle Scholar
  15. 15.
    Kaufman, L. Immunodiagnosis of blastomycosis. In: Al-Doory Y, DiSalvo AF, eds. Blastomycosis, New York: Plenum Medical Book Co., 1992: 123–132Google Scholar
  16. 16.
    Abuodeh, RO, Chester, EM, Scalarone, GM 2004Comparative serological evaluation of 10 Blastomyces dermatitidis yeast phase lysate antigens from different sourcesMycoses47143149PubMedCrossRefGoogle Scholar
  17. 17.
    Klein, BS, Squires, RA, Lloyd, JK, Ruge, DR, Legendre, AM 2000Canine antibody response to Blastomyces dermatitidis WI-1 antigenAm J Vet Res61554558PubMedCrossRefGoogle Scholar
  18. 18.
    Levine, HB, Scalarone, GM, Chaparas, SD 1977Preparation of fungal antigens and vaccines: studies on Coccidioides immitis and Histoplasma capsulatumContrib Microbiol Immunol3106125Google Scholar
  19. 19.
    Scalarone, GM, Levine, HB, Chaparas, SD 1978Delayed hypersensitivity responses of experimental animals to histoplasmin from the yeast and mycelial phases of Histoplasma capsulatumInfect Immun21705713PubMedGoogle Scholar
  20. 20.
    Levine, HB, Scalarone, GM, Campbell, GD, Kelly, PC, Chaparas, SD 1979Histoplasmin-CYL, a yeast phase reagent in skin test studies in humansAm Rev Respir Dis119629636PubMedGoogle Scholar
  21. 21.
    Hurst, SF, Kaufman, L 1992Western immunoblot analysis and serologic characterization of Blastomyces dermatitidis yeast form extracellular antigensJ Clin Microbiol3030433049PubMedGoogle Scholar

Copyright information

© Springer 2006

Authors and Affiliations

  1. 1.Department of Biological SciencesIdaho State UniversityPocatelloUSA

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