, Volume 160, Issue 3, pp 227–234 | Cite as

Detection of Candidadubliniensis in Venezuela

  • Claudia Hartung de  Capriles
  • Sofía Mata-Essayag
  • Celina Pérez
  • Maria Teresa Colella
  • Arantza Roselló
  • Carolina Olaizola
  • Sylvia Magaldi Teresa Abate


Over the past decades there has been a significant increase in fungal infections caused by Candida species, and continues to be common in immunocompromised individuals infected with the human immunodeficiency virus (HIV). Although Candida albicans remains the fungal species most frequently isolated as an opportunistic oral pathogen, other non-albicans are often identified in this cohort of patients, including C. dubliniensis. This yeast is closely related to and shares many phenotypic characteristics with C. albicans. Colonies of these two species appear morphologically identical when not grown on special media. The shared phenotypic characteristics of C. dubliniensis and C. albicans suggest that many C. dubliniensis isolates may have been misidentified as C. albicans in the past. The present studies aim is to recover and identify C. dubliniensis, and presumptive clinical C. albicans, from the oral cavities of HIV-seropositive individuals, comparing conventional media to obtain a simple, low-cost and reliable identification system for C. dubliniensis. A total of 16 isolates (3,98%) had been obtained from 402 HIV infected individuals with recurrent oropharyngitis and were identified as C. dubliniensis. Out of these C. dubliniensis isolates 19% were resistant, with MICs above 64 μg/ml to fluconazole. This constitutes, to the authors knowledge the first recovery of this organism in Venezuela.

Key words

C. dubliniensis phenotypic genotypic HIV Venezuela 


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  1. 1.
    Coleman, DC, Bennett, DE, Sullivan, DJ 1993Candida in HIV infection AIDS. New preventive/new approachesCrit Rev Microbiol196182PubMedGoogle Scholar
  2. 2.
    Fridkin, SK, Jarvis, WR 1996Epidemiology of nosocomial fungal infectionsClin Microbiol Rev9499511PubMedGoogle Scholar
  3. 3.
    Sullivan, DJ, Henman, MC, Moran, GP 1996Molecular genetic approaches to identification, epidemiology and taxonomy of non-albicans Candida speciesJ Med Microbiol44399408PubMedGoogle Scholar
  4. 4.
    Pfaller, MA, Jones, RN, Doern, GV 1999International surveillance of blood stream infections due to Candida species in the European SENTRY program: species distribution and antifungal susceptibility including the investigational triazole and echinocandin agents; SENTRY Participant Group (Europe)Diagn Microbiol Infect Dis351925CrossRefPubMedGoogle Scholar
  5. 5.
    Rangel, F, Wiblin, T, Blumberg, H, Saiman, L, Patterson, J, Rinaldi, M 1999col. National Epidemiology of Mycoses Survey (NEMIS): Variations in Rates of bloodstream infections due to Candida species in seven surgical intensive care units and six neonatal care unitsClin. Inf. Dis29253258Google Scholar
  6. 6.
    Pfaller, M.A, Jones, RN, Doern, GV 2000col. Bloodstream infections due to Candida species: SENTRY antimicrobial surveillance program in North America and Latin America, 1997–1998Antimicrob Agents Chemother4747751Google Scholar
  7. 7.
    Kremery, V, Barnes, AJ 2002Non-albicans Candida spp causing fungaemia pathogenicity, antifungal resistanceJ. Hosp. Infect50242260Google Scholar
  8. 8.
    Mühlschlegel, F, Frosch, M 1999The relevance of Candida species other than Candida albicans as opportunistic pathogensMycoses42194Google Scholar
  9. 9.
    Sullivan, DJ, Moran, GP, Pinjon, E, Al-Mosaid, A, Stokes, C, Vaughan, C, Coleman, DC 2004Comparison of the epidemiology, drug resistance mechanisms and virulence of Candida dubliniensis and Candida albicansFEMS Yeast Research4369376CrossRefPubMedGoogle Scholar
  10. 10.
    Sullivan, D, Coleman, D 1998Candida dubliniensis: Characteristics and IdentificationJ. Clin. Microbiol.36329334PubMedGoogle Scholar
  11. 11.
    Pinjon, E, Sullivan, D, Salkin, I, Shanley, D, Coleman, D 1998Simple, inexpensive, reliable method for differentiation of Candida dubliniensis from Candida albicansJ Clin. Micobiol. Microbiol3620932095Google Scholar
  12. 12.
    Feo, M 1977The nascent culture and its useMycopathologia631856CrossRefGoogle Scholar
  13. 13.
    Odds, FC, Bernearts, R 1994CHROMagar Candida new, differential isolation medium for the presumptive identification of clinically important Candida speciesJ. Clin. Microbiol321923229PubMedGoogle Scholar
  14. 14.
    Staib, P, Morschhäuser, J 1999Chlamydospore formation on Staib agar as a species specific characteristic of Candida dubliniensisMycoses42521524CrossRefPubMedGoogle Scholar
  15. 15.
    Schoofs, A, Odds, FC, Colebunders, R, Ieven, M, Goossens, H 1997Recognition and identification of Candida dubliniensis isolates from HIV patients: use of specialized isolation mediaEur. J. Clin. Micobiol. Infect. Dis16296300CrossRefGoogle Scholar
  16. 16.
    Kirkpatrick, WR, Revankar, SG, McAfee, RX, Fothergill, AW, McCarthy, DI, Sanchez, SE, Cantu, RA, Rinaldi, MG, Patterson, TF 1998Detection of Candida dubliniensis in oropharyngeal samples from human immunodeficiency virus-infected patient in North America by primary CHROagar Candida screening and susceptibility testing of isolatesJ. Clin. Microbiol3630073010PubMedGoogle Scholar
  17. 17.
    Bautista Muñoz, C, Boldo, XM, Villa-Tanaca, L, Hernández-Rodríguez, C 2003Identification of Candida spp. by randomly amplified Polymorphic DNA Analysis and Differentiation between Candida albicans and Candida dubliniensis by direct PCR MethodsJ. Clin Microbiol4141420CrossRefPubMedGoogle Scholar
  18. 18.
    Soll, DR 2000The Ins and Outs of DNA Fingerprinting the Infectious FungiClin. Microbiol. Rev13332370CrossRefPubMedGoogle Scholar
  19. 19.
    Lasker BA., Page LS., Lott TJ. Nucleotide sequence of a repetitive DNA from the opportunistic yeast, Candida albicans. Unpublished. Submitted to Center for Disease Control, Division of Bacterial & Mycotic Disease, BLDG. 5 B/12G11, 1600 Clifton RD. Atlanta, GA 30333, USAGoogle Scholar
  20. 20.
    National Committee for Clinical Laboratory Standards. Reference method for broth dilution antifungal susceptibility testing of yeasts. Approved standard M27-A2. 2002. Wayne, Pa, National Committee for Clinical Laboratory Standards. 2002Google Scholar
  21. 21.
    Magaldi, S, Mata, S, Hartung, C, Verde, G, Deibis, L, Roldán, Y, Marcano, C 2000In vitro susceptibility of 137 Candida sp. isolates from HIV positive patients to several antifungal drugsMycopath1496368CrossRefGoogle Scholar
  22. 22.
    Magaldi, S, Rios, A, Hartung, C, Verde, G, Spencer, L, Mata, S 2001In vitro susceptibility to fluconazole of Candida spp. isolates comparing three different methodsJ. Mycol. Med.11123126Google Scholar
  23. 23.
    Magaldi, S, Mata-Essayag, S, Hartung Capriles, C, Perez, C, Colella, MT, Olaizola, C, Ontiveros, Y 2004Well diffusion for antifungal susceptibility testingI. J. I. D83945Google Scholar
  24. 24.
    Harbarth, S, Samore, MH, Lichtenberg, D 2000col. Prolonged antibiotic prophylaxis after cardiovascular surgery and its effect on surgical site infections and antimicrobial resistanceCirculation10129162921PubMedGoogle Scholar
  25. 25.
    Pfaller, MA 1995Epidemiology of candidiasisJ. Hosp. Infect30329338CrossRefPubMedGoogle Scholar
  26. 26.
    Pfaller, MA, Diekema, DJ 2002Role of sentinel surveillance of candidemia: trends in species distribution and antifungal susceptibilityJ. Clin. Microbiol.4035517CrossRefPubMedGoogle Scholar
  27. 27.
    Pfaller, MA, Diekema, DJ, Messer, SA, Boyken, L, Hollis, RJ 2003Activities of Fluconazole and Voriconazole against 1.586 Recent Clinical Isolates of Candida Species Determined by Broth Microdilution, Disk Diffusion, and E-test Methods: Report from The ARTEMIS Global Antifungal Susceptibility Program, 2001J. Clin. Microbiol414401446CrossRefGoogle Scholar
  28. 28.
    Tintelnot, K, Haase, G, Seibold, M, Bergmann, F, Staemmler, FT, Naumann, D 2000Evaluation of phenotypic makers for selection and identification of Candida dubliniensisJ. Clin. Microbiol3815991608PubMedGoogle Scholar
  29. 29.
    Ponton, J, Ruchel, R, Clemons, KV, Coleman, DC, Grillot, R, Guarro, J, Aldebert, D, Ambroise-Thomas, P, Cano, J, Carrillo-Muñoz, AJ, Gene, J, Pinel, C, Stevens, DA, Sullivan, DJ 2000Emerging pathogensMed. Mycol3815991608Google Scholar
  30. 30.
    Lasker, BA, Elie, CM, Lott, TJ, Espinel-Ingroff, A, Gallagher, L, Kuykendall, RJ, Keilum, ME, Pruitt, WR, Warnok, DW, Rimland, D, McNeil, MM, Reiss, E 2001Molecular epidemiology of Candida albicans strains isolated from the oropharynx of HIV-positive patients at successive clinic visitsMed. Mycol39341352PubMedGoogle Scholar
  31. 31.
    Prasad, R, Panwar, SL 2002Smriti. Drug resistance in yeasts-an emerging scenarioAdv. Microb Physiol46155201CrossRefPubMedGoogle Scholar
  32. 32.
    Borg-von Zepelin, M, Niedrhaus, T, Gross, U, Seibold, M, Monod, M, Tintelnot, K 2002Adherence of different Candida dubliniensis isolates in the presence of fluconazoleAIDS612371244CrossRefGoogle Scholar
  33. 33.
    Pfaller, MS, Messer, SA, Gee, S, Joly, S, Pujol, C, Sullivan, DJ, Coleman, DC, Soll, DR 1999In vitro susceptibilities of Candida dubliniensis isolates tested against the new triazoles and echinocandin antifungal agentsJ. Clin. Microbiol37870872PubMedGoogle Scholar
  34. 34.
    Martinez, M, Lopez-Ribot, JL, Kirkpatrick, WR, Coco, BJ, Bachmann, SP, Patterson, TF 2002Replacement of Candida albicans with C. dubliniensis in human immunodeficiency virus.-infected patients with oropharyngeal candidiasis treated with fluconazoleJ. Clin. Microbiol.4031353139CrossRefPubMedGoogle Scholar
  35. 35.
    Moran, GP, Sullivan, DJ, Henman, MC, McCreary, CE, Harrington, BJ, Shanley, DB, Coleman, DC 1997Antifungal drug susceptibilities of oral Candida dubliniensis isolates form human immunodeficiency virus (HIV)-infected and non-HIV-infected subjects and generation of stable fluconazole-resistant derivatives in vitroAntimicrob. Agents. Chemther.41617623Google Scholar
  36. 36.
    Jabra-Rizk, MA, Falkler, WA, Merz, WG 1999coaggregation of C.dubliniensis with Fusobacterium nucleosumJ. Clin. Microbiol3714641468PubMedGoogle Scholar
  37. 37.
    Sullivan, D, Haynes, K, Billi, J, Boerlin, P, Rodero, L, Lloyd, S, Henman, M, Coleman, D 1997Widespread geographic distribution of oral Candida dubliniensis strains in human immunodeficiency virus-infected individualsJ. Clin. Microbiol.35960964PubMedGoogle Scholar
  38. 38.
    Jabra-Rizk, MA, Falkler, WA, Merz, WG, Baqui, AM, Kelley, JI, Meiller, TF 2000Retrospective identification, characterization of Candida dubliniensis isolates among Candida albicans clinical laboratory isolates from Human Immunodeficiency Virus (HIV)-infected, non-HIV-infected individualsJ. Clin. Microbiol3824232426PubMedGoogle Scholar
  39. 39.
    Martinez, M, López-Ribot, L, Kirkpatrick, W, Brent, J, Coco, BS, Bachmann, S, Patterson, T 2002Replacement of Candida albicans with C.dubliniensis in Human Immunodeficiency Virus-Infected Patients with Oropharyngeal Candidiasis Treated with FluconazoleJ. Clin. Microbiol4031353139CrossRefPubMedGoogle Scholar
  40. 40.
    Jabra-Rizk, MA, Baqui, AM, Kelly, J, Falker, WA, Merz, WG,Jr., Meiller, TF 1999Identification of C.dubliniensis in a prospective study of patients in United StatesJ. Clin. Microbiol37321326PubMedGoogle Scholar
  41. 41.
    Odds, F, Nuffel, L, Dams, G 1998Prevalence of Candida dubliniensis isolates in a yeast stock collectionJ. Clin. Microbiol3628692873PubMedGoogle Scholar
  42. 42.
    Kurzai, O, Korting, H, Harmsen, D, Bautsch, W, Monitor, M, Frosch, M, Mühlschlegel, F 2000Molecular and phenotypic identification of the yeast pathogen Candida dubliniensisJ. Mol. Med78521529CrossRefPubMedGoogle Scholar
  43. 43.
    Pincus, DH, Coleman, DC, Pruit, WR, Padhye, AA, Salkin, IF, Geimer, M, Bassel, A, Sullivan, DJ, Clarke, M, Hearn, V 1999Rapid Identification of Candida dubliniensis with Commercial yeast Identification SystemsJ. Clin. Microbiol.3735333539PubMedGoogle Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  • Claudia Hartung de  Capriles
    • 1
  • Sofía Mata-Essayag
    • 1
  • Celina Pérez
    • 1
  • Maria Teresa Colella
    • 1
  • Arantza Roselló
    • 1
  • Carolina Olaizola
    • 1
  • Sylvia Magaldi Teresa Abate
    • 2
  1. 1.Sección de Micología MédicaInstituto de Medicina Tropical, Universidad Central deVenezuela
  2. 2.Sección de Biología MolecularInstituto de Medicina Tropical, Universidad Central deVenezuela
  3. 3.Sección de Micología Médica, Instituto de Medicina TropicalCiudad Universitaria, Los ChaguaramosCaracasVenezuela

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