Mycopathologia

, Volume 160, Issue 4, pp 303–314

Differential Expression of Chitin Synthase (CHS) and Glucan Synthase (FKS) Genes Correlates with the Formation of a Modified, Thinner Cell Wall in in vivo-produced Beauveria bassiana Cells

  • Aurélien Tartar
  • Alexandra M. Shapiro
  • Dancia W. Scharf
  • Drion G. Boucias
Article

Abstract

During infection (in vivo), the entomopathogenic fungus Beauveria bassiana produces yeast-like cells that are surrounded by modified cell walls. These modifications have been related to the fungus ability to limit recognition by the host defense system. The composition of the in vivo cell wall was analyzed using a combination of cytochemical and molecular techniques. The in vivo cell walls still contained both chitin and 1,3-β-glucan, but they were significantly thinner than in vitro cell walls (50–60 nm versus 100–160 nm, respectively). The difference in cell wall thickness was correlated with transcriptional regulation of cell wall-related genes: quantitative RT-PCR reactions demonstrated that B. bassiana chitin synthase (CHS) and glucan synthase (FKS) genes are down regulated in vivo. These analyses indicate that in vivo-triggered phenotypic modifications, including cell wall adjustments, are controlled by molecular mechanisms that include regulation of gene expression at the transcriptional level.

Keywords

AY743592 AY743593 CF350282 dimorphism fungal cell wall host-pathogen interactions 

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Copyright information

© Springer 2005

Authors and Affiliations

  • Aurélien Tartar
    • 1
  • Alexandra M. Shapiro
    • 2
  • Dancia W. Scharf
    • 3
  • Drion G. Boucias
    • 3
  1. 1.Department of BiologyClark UniversityWorcesterUSA
  2. 2.Center for Medical, Agricultural and Veterinary EntomologyUSDA, ARSGainesville
  3. 3.Department of Entomology and NematologyUniversity of FloridaGainesvilleUSA

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