Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

First genomic microsatellite markers developed for Platonia insignis (Clusiaceae), a Brazilian fruit tree


Platonia insignis is a fruit tree native of Brazil with allogamous and asexual reproduction. The production of fruits is mainly obtained by exploitation of natural populations and the impact of genetic structuring on plant production may be evaluated. For this purpose, codominant and multiallelic markers such as microsatellite are the most suitable, but they need to be developed for this species. Thus, the aim of this work was to develop and validate microsatellite markers for P. insignis. We used Roche 454 GS FLX sequencing platform of a single P. insignis genotype and 1702 microsatellite sequences were identified. Based on some pre-requisites, we could develop 50 primer pairs to be tested. Twenty-two primer pairs successfully amplified fragments and they were tested in 31 genotypes of P. insignis that belong to a germplasm bank and were sampled in the northeast of Pará State, Brazil. Thirteen primers were polymorphic and the number of alleles per loci varied from 5 (PI18 and PI27) to 2 (PI08, PI25, PI31, PI33 and PI 37). Expected heterozygosity (HE) varied from 0.74 (PI27) to 0.12 (PI31) and observed heterozygosity (HO) varied from 1.00 (PI25) to 0.00 (PI08, PI31, PI33 and PI37). Principal coordinates could separate the genotypes of P. insignis in clusters and we can conclude that the primers can estimate the genetic diversity of P. insignis populations.

This is a preview of subscription content, log in to check access.

Fig. 1


  1. 1.

    Ferreira MSG, Medina G (2004) Bacuri: gold in the market, delicious in the mouth. In: Alexiades M, Shanley P (eds) Productos forestales, medios de subsistencia y conservácion: estudios de caso sobre sistemas de manejo de productos forestales no moderables. CIFOR, Bogor, pp 5–8

  2. 2.

    Hammer O, Harper DAT, Ryan PD (2001) PAST: paleontological statistics software package for education and data analysis. Palaeontol Electron 4:1–9

  3. 3.

    Homma AKO, de Menezes AJE, de Carvalho JEU, de Matos GB (2018) Manejo e plantio de bacurizeiros (Platonia insignis Mart.): a experiência no manejo e domesticação de um recurso da biodiversidade amazônica. Inc Soc 12:48–57

  4. 4.

    Kalia RK, Raí MK, Kalia S, Rohtas S, Dhawan AK (2011) Microsatellite markers: an overview of the recent progress in plants. Euphytica 177:309–334

  5. 5.

    Maués MM, Venturieri GC (1996) Ecologia da polinização do bacurizeiro (Platonia insignis Mart.) Clusiaceae. Boletim de pesquisa 170, Embrapa Amazônia Oriental, Belém, Pará. 24p.

  6. 6.

    Paetkau D, Strobeck C (1994) Microsatellite analysis of genetic variation in black bear populations. Mol Ecol 3:489–495

  7. 7.

    Peakall R, Smouse PE (2012) GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research-an update. Bioinformatics 28:2537–2539

  8. 8.

    Pontes LCG, Moura EF, Moura MF, Rodrigues SM, Oliveira MSP, Carvalho JEU, Therrier J (2017) Molecular characterization of progenies of bacurizeiro (Platonia insignis) from Marajó Island, northeastern Amazon. Acta Amaz 47:293–300. https://doi.org/10.1590/1809-4392201701302

  9. 9.

    Rogez H, Buxant R, Mignolet E, Souza JNS, Silva EM, Larondelle Y (2004) Chemical composition of the pulp of three typical Amazonian fruits: araça-boi (Eugenia stipitata), bacuri (Platonia insignis) and cupuaçu (Theobroma grandiflorum). Eur Food Res Technol 218:380–384

  10. 10.

    Rosen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. Methods Mol Biol 132:365–386

Download references


The authors thank Brazilian Agricultural Research Corporation (Embrapa), Process No. for financial support and the Coordination for the Improvement of Higher Education Personnel (CAPES) for scholarship grants for the first and second authors.

Author information

Correspondence to Elisa Ferreira Moura.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest directly or indirectly and informed consent to publish this study and that the manuscript complies with the ethical standards of the journal.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Paraense, L.C.R., Pena, D.N., Darnet, S.H. et al. First genomic microsatellite markers developed for Platonia insignis (Clusiaceae), a Brazilian fruit tree. Mol Biol Rep (2020). https://doi.org/10.1007/s11033-020-05301-0

Download citation


  • Simple sequence repeats
  • Genetic diversity
  • Oligonucleotides
  • Next generation sequencing