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Molecular Biology Reports

, Volume 47, Issue 2, pp 1361–1369 | Cite as

Hsa-miR-584-5p as a novel candidate biomarker in Turkish men with severe coronary artery disease

  • Neslihan CobanEmail author
  • Dilek Pirim
  • Aycan Fahri Erkan
  • Berkcan Dogan
  • Berkay Ekici
Original Article
  • 64 Downloads

Abstract

Coronary artery disease (CAD) is still the preliminary cause of mortality and morbidity in the developed world. Identification of novel predictive and therapeutic biomarkers is crucial for accurate diagnosis, prognosis and treatment of the CAD. The aim of this study was to detect novel candidate miRNA biomarker that may be used in the management of CAD. We performed miRNA profiling in whole blood samples of angiographically confirmed Turkish men with CAD and non-CAD controls with insignificant coronary stenosis. Validation of microarray results was performed by qRT-PCR in a larger cohort of 62 samples. We subsequently assessed the diagnostic value of the miRNA and correlations of miRNA with clinical parameters. miRNA-target identification and network analyses were conducted by Ingenuity Pathway Analysis (IPA) software. Hsa-miR-584-5p was one of the top significantly dysregulated miRNA observed in miRNA microarray. Men-specific down-regulation (p = 0.040) of hsa-miR-584-5p was confirmed by qRT-PCR. ROC curve analysis highlighted the potential diagnostic value of hsa-miR-584-5p with a power area under the curve (AUC) of 0.714 and 0.643 in men and in total sample, respectively. The expression levels of hsa-miR-584-5p showed inverse correlation with stenosis and Gensini scores. IPA revealed CDH13 as the only CAD related predicted target for the miRNA with biological evidence of its involvement in CAD. This study suggests that hsa-miR-584-5p, known to be tumor suppressor miRNA, as a candidate biomarker for CAD and highlighted its putative role in the CAD pathogenesis. The validation of results in larger samples incorporating functional studies warrant further research.

Keywords

Atherosclerosis miRNA Microarray IPA T-Cadherin Stenosis 

Notes

Acknowledgements

This study was supported by The Research Support Unit of Istanbul University as the Project Numbers: 51865 and 47372.

Author contributions

NC, DP designed and conducted the experiments. AFE. and BE contributed materials. NC, DP, BD contributed to data analysis and wrote the manuscript. NC, DP, BD, AFE and BE interpreted the results. All authors reviewed the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that there are no conflicts of interest.

Supplementary material

11033_2019_5235_MOESM1_ESM.docx (34 kb)
Supplementary file1—Supplementary Tables 1 to 5 (DOCX 34 kb)
11033_2019_5235_MOESM2_ESM.tif (57.4 mb)
Supplementary file2—Supplementary Figure 1 (TIF 58.818 kb)
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Supplementary file3—Supplementary Figure 2 (TIF 96,362 kb)
11033_2019_5235_MOESM4_ESM.tif (92.3 mb)
Supplementary file4—Supplementary Figure 3 (TIF 94,488 kb)
11033_2019_5235_MOESM5_ESM.tif (292.5 mb)
Supplementary file5—Supplementary Figure 4 (TIF 299,530 kb)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Neslihan Coban
    • 1
    Email author
  • Dilek Pirim
    • 2
  • Aycan Fahri Erkan
    • 3
  • Berkcan Dogan
    • 4
    • 5
  • Berkay Ekici
    • 3
  1. 1.Department of Genetics, Aziz Sancar Institute for Experimental MedicineIstanbul UniversityIstanbulTurkey
  2. 2.Faculty of Arts & Science, Department of Molecular Biology and GeneticsBursa Uludag UniversityBursaTurkey
  3. 3.Faculty of Medicine, Department of CardiologyUfuk UniversityAnkaraTurkey
  4. 4.Institute of Graduate Studies in Sciences, Department of Molecular Biology and GeneticsIstanbul UniversityIstanbulTurkey
  5. 5.Department of Medical GeneticsBursa Uludag UniversityBursaTurkey

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