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Polymorphisms of LAP3 gene and their association with the growth traits in the razor clam Sinonovacula constricta

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Abstract

Leucine aminopeptidase 3 (LAP3) is an important proteolytic enzyme that catalyzes the hydrolysis of leucine residues from the amino termini of protein or peptide substrates and plays a critical role in protein metabolism and growth. In the present study, we investigated the full-length cDNA sequence of the LAP3 gene in Sinonovacula constricta (ScLAP3) using expressed sequence tags and rapid amplification of cDNA ends. The full-length ScLAP3 cDNA was 2885 bp, with a 1560 bp open reading frame encoding 519 amino acids. Sequence analysis revealed that ScLAP3 shared 70.9% identity with LAP3 from the blood clam Tegillarca granosa and 62.0–68.0% with other species. ScLAP3 was expressed in all six tested tissues, with significantly higher expression levels in the foot compared with mantle, adductor muscle, liver, gills, and siphon tissues in adults (P < 0.01). In the eight developmental stages, ScLAP3 expression gradually increased, with significantly higher levels in D-shaped larvae compared with other developmental stages (P < 0.01), suggesting that it may be involved in the formation of certain organs during early development. Association analysis identified three shared single nucleotide polymorphisms (SNPs), c.1073A > G, c.1139C > T and c.1154A > G in exons of ScLAP3 gene from 177 individuals of two groups, one selective strain and one wild population, which had significant effects on growth traits of S. constricta. The results provided candidate genetic markers to assist selective breeding of razor clams toward improved growth.

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Acknowledgements

This work was financially supported by the National Key Research and Development Program of China (2018YFD0901405), Zhejiang Major Program of Science and Technology (2016C02055-9), Modern Agro-industry Technology Research System (CARS-49), Ningbo Natural Science Foundation (2016A610230) and Zhejiang Provincial Top Key Discipline of Biological Engineering (1740004014).

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Correspondence to Yinghui Dong.

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Yao, H., Liu, C., Lin, D. et al. Polymorphisms of LAP3 gene and their association with the growth traits in the razor clam Sinonovacula constricta. Mol Biol Rep 47, 1257–1264 (2020). https://doi.org/10.1007/s11033-019-05231-6

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Keywords

  • Leucine aminopeptidase 3
  • SNP
  • Sinonovacula constricta
  • Growth traits