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Development of microsatellite markers for the threatened species Coleocephalocereus purpureus (Cactaceae) using next-generation sequencing

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Abstract

Ten microsatellite loci were developed and validated for the endangered cactus species Coleocephalocereus purpureus. The markers were obtained from sequences generated by whole genome shotgun sequencing approaches. A testing group of 36 specimens of the main population were genotyped and all described markers presented suitable outcomes to population genetic studies, showing polymorphic status for C. purpureus testing group with clean and reproducible amplification. No evidence for scoring errors, null alleles or linkage disequilibrium was detected. Number of alleles per locus ranged from 3 to 6 and expected heterozygosity ranged from 0.78 to 0.99. These new microsatellite loci are suitable to be used in future diversity and structure population studies of C. purpureus.

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Acknowledgements

The authors thank the Program for Technological Development in Tools for Health-PDTIS FIOCRUZ for use of its facilities and Nativa Serviços Ambientais Ltda for logistic support during collections of biological material.

Funding

Funding was provided by Instituto Federal de Minas Gerais [Grant No. Edital de Pesquisa Aplicada (Edital 104-2016)] and FAPEMIG [Grant No. Processo: MPR-01008-16]

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Correspondence to Gustavo Augusto Lacorte.

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Fraga, D.A., de Carvalho, A.F., Santana, R.S. et al. Development of microsatellite markers for the threatened species Coleocephalocereus purpureus (Cactaceae) using next-generation sequencing. Mol Biol Rep 47, 1485–1489 (2020) doi:10.1007/s11033-019-05213-8

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Keywords

  • Microsatellites
  • Population genetics
  • Cactus
  • Endangered species