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Molecular Biology Reports

, Volume 47, Issue 2, pp 1013–1020 | Cite as

Identification and characterization of microRNAs in phloem and xylem from ramie (Boehmeria nivea)

  • Fang Liu
  • Yinghong Tang
  • Qingquan Guo
  • Jianrong ChenEmail author
Original Article
  • 51 Downloads

Abstract

Ramie (Boehmeria nivea) is a widely cropped species in southern China due to its high economic value of natural fiber for industry. Development of phloem and xylem is key evidence for generating fiber. However, the MicroRNA (miRNA) profiles of phloem and xylem in ramie have not been reported yet. miRNA belong to a small RNA family which has been recognized as an important regulator for various biological processes. In the present study, we aimed to identify differently expressed miRNAs between phloem and xylem in adult ramie. The results showed that 137 and 122 unique conserved miRNAs were identified from phloem and xylem libraries, respectively. Meanwhile, 4 novel miRNAs were identified from ramie by miRDeep2. Of these miRNAs, 77 conserved miRNAs in ramie were differentially expressed. Among the differentially expressed miRNAs, 44 miRNAs and 33 miRNAs were up-regulated and down-regulated in phloem compared to that in xylem, respectively. The functions of differentially expressed miRNAs were associated with regulating the development and differentiation of phloem and xylem. The present study provides a glance of miRNA profiles for further understanding of miRNA role in ramie development.

Keywords

Boehmeria nivea Phloem Xylem microRNA Small RNA sequencing 

Notes

Funding

This research was supported by the National Natural Science Foundation of China (No. 31301374).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Research involving human and animal rights and informed consent

This article does not contain any studies with human subjects or animals performed by any of the authors.

Supplementary material

11033_2019_5193_MOESM1_ESM.xlsx (43 kb)
Supplementary material 1 (XLSX 42 kb)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Biotechnology and Environmental ScienceChangsha UniversityChangshaChina

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