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Sequence characteristics of Medicago truncatula cyclophilin family members and function analysis of MsCYP20-3B involved in axillary shoot development

  • Lingqiao Ge
  • Kun Zhang
  • Xiaohui Cao
  • Yinyin Weng
  • Bei Liu
  • Peisheng Mao
  • Xiqing MaEmail author
Original Article
  • 42 Downloads

Abstract

Cyclophilins (CYPs) belonging to the immunophilin family are present in all organisms and widely distributed in various cells associated with the activity of peptidyl-prolyl cis/trans isomerase. Plant CYPs are members of a multi-gene family and are involved in a series of biological processes. However, little is known about their structure, evolution, developmental expression and functional analysis in Medicago truncatula. In this study, a total of 33 CYP genes were identified and found to be unevenly distributed on eight chromosomes. Among them, 21 are single-domain and 12 are multi-domain proteins, and most were predicted to be localized in the cytosol, nucleus or chloroplast. Phylogenetic and gene structure analysis revealed seven segmental gene pairs, indicating that segmental duplication probably made a large contribution to the expansion of MtCYP gene family. Furthermore, gene expression analysis revealed that about 10 MtCYP genes (were) highly expressed involved in vegetative and reproduction tissues in M. truncatula, and MsCYP20-3B was mainly upregulated in stems, leaves and flower buds in alfalfa (Medicago sativa). Overexpression of MsCYP20-3B was shown to regulate axillary shoot development associated with higher jasmonic acid and abscisic acid contents in M. truncatula. Our study suggests the importance of the CYP genes family in development, reproduction and stress responses, and provides a reference for future studies and application of CYP genes for alfalfa genetic improvement.

Keywords

Cyclophilin genes Medicago truncatula Phylogenetic analysis Gene expression pattern Transgenic plants Alfalfa 

Notes

Acknowledgements

This work was supported by the Fundamental Research Funds for the Central Universities (NSFC, 2018QC144).

Author contributions

LG designed and performed the experiments; LG and KZ analyzed the data and wrote the manuscript; XM and PM designed and supervised the working and edited the manuscript. XC, YW and BL involved in performing the experiments. All authors read and approved the final manuscript.

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.

Supplementary material

11033_2019_5183_MOESM1_ESM.xlsx (19 kb)
Supplementary file 1 (XLSX 20 kb)
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Supplementary file 2 (XLSX 28 kb)
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Supplementary file 3 (DOCX 24 kb)
11033_2019_5183_MOESM4_ESM.jpg (2.9 mb)
Supplementary file 4 Fig. S1 The alignment of amino acid sequences of MtCYP20-3B, MsCYP20-3B and AtCYP20-3 using DNAman software (JPG 2999 kb)
11033_2019_5183_MOESM5_ESM.xlsx (10 kb)
Supplementary file 5 (XLSX 11 kb)

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© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.College of Grassland Science and TechnologyChina Agricultural UniversityBeijingChina
  2. 2.Key Laboratory of Pratacultural ScienceBeijingChina

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