A combination of Citrus reticulata peel and black tea inhibits migration and invasion of liver cancer via PI3K/AKT and MMPs signaling pathway

  • Shuai Wen
  • Lingli Sun
  • Ran An
  • Wenji Zhang
  • Limin Xiang
  • Qiuhua Li
  • Xingfei Lai
  • Mengen Huo
  • Dongli LiEmail author
  • Shili SunEmail author
Original Article


Liver cancer, one of the most common malignancies, is the second leading cause of cancer death in the world. The citrus reticulate peel and black tea have been studied for their beneficial health effects. In spite of the many studies have been reported, the underlying molecular mechanisms underlying its health benefits are still not fully understood. In present study, we developed a unique citrus reticulate peel black tea (CRPBT) by combined citrus reticulate peel and black tea and assessed its active ingredients, anti-oxidant and anti-liver cancer effects in vitro. The results suggested that CRPBT exhibited antioxidant capacity and effectively inhibited proliferation and migration of liver cancer cells in a dose- and time- dependent manner. Mechanistically, CRPBT significantly down-regulated phosphorylation of PI3K and AKT, and up-regulated the ratio of Bax/Bcl-2, and suppressed the expression of MMP2/9, N-cadherin and Vimetin proteins in liver cancer cells. Taken together, CRPBT has good effect on inhibiting migration, invasion, proliferation, and inducing apoptosis in liver cancer cells.


Citrus reticulate peel black tea Liver cancer Migration Invasion Signaling pathway 



This work was supported by the National Natural Science Foundation of China (Nos. 81803236, 31800295, 81903319). Guangdong Science and Technology program (Nos. 2017A070702004, 2016B090918118, 2017A020224015, 2018KJYZ002), Natural Science Foundation of Guangdong Province (Nos. 2017A030310504 and 2016A030313006), ShaoGuan Science and Technology Program (No. 2018CS11902), Science and Technology Board of Yingde (NO. JHXM2018029), the Foundation for Department of Education of Guangdong Province (Nos. 2016KCXTD005 and 2017KSYS010), the Youth Foundation of Wuyi University (No. 2017td01), the President Foundation of Guangdong Academy of Agricultural Sciences (Nos. 201534 and 201720).

Author contributions

SS and DL conceived and designed the experiments. SW performed the experiments. SW and LS analyzed the data. LX, WZ, XL, QL, RA and MH contributed reagents. SW wrote the paper, and LS and SS critically revised the manuscript. All authors approved the final version of the manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11033_2019_5157_MOESM1_ESM.docx (851 kb)
Supplementary material 1 (DOCX 850 kb)


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.School of Biotechnology and Health SciencesWuyi UniversityJiangmenPeople’s Republic of China
  2. 2.Tea Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of Tea Resources Innovation & UtilizationGuangzhouPeople’s Republic of China
  3. 3.International Healthcare Innovation Institute (Jiangmen)JiangmenPeople’s Republic of China

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