Identification of ribosomal sites and karyotype analysis in Festuca ulochaeta Steud. and Festuca fimbriata Ness., grasses native to Brazil

  • Marco Túlio Mendes Ferreira
  • Ana Luisa Arantes Chaves
  • Laiane Corsini Rocha
  • Leonardo Nogueira da Silva
  • Eliane Kalthuk-Santos
  • Vania Helena TechioEmail author
Original Article


Festuca L. has more than 600 perennial species described, which makes it the largest genus within the family Poaceae. In Brazil, only two native species of Festuca have been described, for which cytogenetic studies need to be strengthened: Festuca ulochaeta and Festuca fimbriata. The aim of this study was to characterize the karyotypes of F. ulochaeta and F. fimbriata based on the mapping of rDNA sites. The FISH was performed with 35S and 5S rDNA probes. Both species have 42 chromosomes, of which 36 were metacentric and six were submetacentric. Festuca fimbriata has two pairs of 35S rDNA sites, one located on the metacentric pair 4, in an interstitial position, and one at the submetacentric pair 14 in the proximal position. Festuca ulochaeta has one pair of 35S rDNA in interstitial-proximal position in the metacentric pair 3. Both species showed 5S rDNA sites only on chromosome pair 21 in the terminal position of the short arm. The analysis of the chromosomal characteristics indicates that these species have a symmetrical karyotype and allopolyploid origin.


Poaceae Polyploidy FISH Ribosomal DNA 



The authors thank the National Council for Scientific and Technological Development (CNPq), the Foundation for Research Support of the State of Minas Gerais (FAPEMIG) and the Coordination for the Improvement of Higher Education Personnel (CAPES) for financial support.

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interest.


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of BiologyFederal University of Lavras - UFLALavrasBrazil
  2. 2.Department of BiologyFederal University of Rio Grande do Sul - UFRGSPorto AlegreBrazil

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