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Molecular Biology Reports

, Volume 46, Issue 6, pp 6557–6563 | Cite as

CASP8 (rs3834129) and CASP3 (rs4647601) polymorphisms in oropharynx cancer risk, tumor cell differentiation, and prognosis in a cohort of the Brazilian population

  • Gabriela Arielo Tortorelli
  • Caroline Torricelli
  • Juliana Carron
  • Ericka Francislaine Dias Costa
  • Leisa Lopes-Aguiar
  • Bruna Fernandes Carvalho
  • José Augusto Rinck-Junior
  • Fernanda Viviane Mariano
  • Albina Messias Almeida Milani Altemani
  • Carmen Silvia Passos Lima
  • Gustavo Jacob LourençoEmail author
Short Communication
  • 69 Downloads

Abstract

The objective of this research was to assess the association of genetic polymorphisms related to intrinsic apoptosis pathway CASP8 rs3834129 and CASP3 rs4647601 with the risk, clinical and pathological aspects, and survival of oropharynx squamous cell carcinoma (OPSCC) patients that received cisplatin and radiotherapy. The genotypes were identified in 198 patients with OPSCC and 200 controls using polymerase chain reaction methods. Chi square or Fisher’s exact test and logistic regression were applied for the detection of differences between groups. Patients’ genotypes were statistically evaluated considering the event-free survival and overall analysis using Kaplan–Meier estimate and Cox regression. CASP3 rs4647601 GG genotype (44.4% vs. 30.0%, p = 0.03) and G allele (63.9% vs. 55.5%, p = 0.04) were more common in patients with OPSCC than in controls. Carriers of GG genotype and G allele were under 1.78-fold and 1.40-fold increased risk of OPSCC than others, respectively. The frequency of CASP8 rs3834129 DD genotype was higher in patients with OPSCC with poorly differentiated or undifferentiated tumors when compared to others (34.5% vs. 16.1%, p = 0.02). No influence of CASP8 and CASP3 polymorphisms on OPSCC patients’ survival was seen in this study. Our results indicate that inherited genetic variants in the intrinsic apoptosis pathway related to CASP3 rs4647601 and CASP8 rs3834129 polymorphisms may be an important determinant of OPSCC risk and tumor cell differentiation.

Keywords

Oropharynx cancer Apoptosis CASP8 CASP3 Polymorphism Risk Tumor cell differentiation Prognosis 

Notes

Acknowledgements

We would like to thank Dr. Tiago Chedraoui Silva for assistance with bioinformatics analysis. This study was funded by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (PIBIC 2014).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Supplementary material

11033_2019_5107_MOESM1_ESM.docx (40 kb)
Supplementary material 1 (DOCX 39 kb)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Gabriela Arielo Tortorelli
    • 1
  • Caroline Torricelli
    • 1
  • Juliana Carron
    • 1
  • Ericka Francislaine Dias Costa
    • 1
  • Leisa Lopes-Aguiar
    • 1
  • Bruna Fernandes Carvalho
    • 1
  • José Augusto Rinck-Junior
    • 2
  • Fernanda Viviane Mariano
    • 3
  • Albina Messias Almeida Milani Altemani
    • 3
  • Carmen Silvia Passos Lima
    • 1
    • 2
  • Gustavo Jacob Lourenço
    • 1
    Email author
  1. 1.Laboratory of Cancer Genetics, Faculty of Medical SciencesUniversity of CampinasCampinasBrazil
  2. 2.Department of Internal Medicine, Faculty of Medical SciencesUniversity of CampinasCampinasBrazil
  3. 3.Department of Pathology, Faculty of Medical SciencesUniversity of CampinasCampinasBrazil

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