Possible synergistic effect of apoE and LRP1 genotypes on metabolic syndrome development in Serbian patients
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The modern way of life contributes to the higher frequency of a complex state medically called metabolic syndrome (MetS), which is an inevitable consequence of several most common diseases of modern civilization. Patients with MetS have three times higher risk of experiencing a heart attack or a stroke and twice higher possibility to die from them. Serbia holds the infamous third place in Europe in mortality from heart disease, just behind Russia and Ukraine. The study explores the correlation of every combination of genotypes of apoE (apolipoprotein E) and LRP1 (low density receptor- related protein 1) genes with presence of MetS, and the connection with each anthropometric and biochemical parameter in both tested groups. Study demonstrates the impact of genotype combinations on the emergence and development of the MetS in Serbia. 63 patients and 30 controls were included in the study, aged from 19 to 65. Each person genotype was determined by the polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) profile. Odds ratio (OR) values showed that the presence of apoE e3e4/LRP1 CC genotype combination of genotypes in patients multiplies the chance (7.6 times) for the occurrence of the MetS in comparison to the presence of other genotype combinations. Determining the genetic basis of MetS is one of the necessary steps in the prevention of disease, saving the cost of treatment, and in the design of targeted therapies.
KeywordsMetabolic syndrome LRP1 gene variants apoE gene variants
All the study participants made possible completion of this study. The study was supported by the Ministry of Education, Science and Technological Development, Republic of Serbia, Grant No. 179006.
Study conception and design: NV, ES, MD, KS. Data analysis and interpretation: NV. Drafting the article: ID, DO, IB. Critical revision of the article: ES, MD, IB, KS, LS, DO. Performed analysis on all samples, interpreted data, wrote manuscript, revised the article and acted as corresponding author: NV.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
Ethical board of Clinical Center Vojvodina, Serbia and Ethical board of Medical Faculty Novi Sad, University of Novi Sad, Serbia approved this study. The study was performed according to the Declaration of Helsinki.
Signed informed consent was obtained from all individual participants included in the study.
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