CCL5 protein level: influence on breast cancer staging and lymph nodes commitment
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Many tumor cells express chemokines and chemokine receptors, and these molecules can contribute to distinct modes of metastasis processes. It is known that they play a crucial role in breast cancer (BC) tumorigenesis and progression. Considering this, it was investigated a possible role for C–Chemokine receptor type 5(CCR5) polymorphism (rs333/delta32) by conventional polymerase chain reaction (PCR) and CCL5 (C–C motif chemokine ligand 5) protein level by immunosorbent assay (ELISA) in 47 BC patients (resulting in 47 tumoral tissue samples and 47 adjacent normal tissue samples). There was a significant difference between CCL5 level in tumoral and adjacent normal tissues for the same BC patients (p < 0.0001). A significant association was also found for CCL5 level in relation to lymph nodes commitment (p = 0.03). Likewise, there was a significant difference in CCL5 level from tumor tissue of stage III in relation to stage I (p < 0.02). On the other hand, it was verified that CCR5-delta32 polymorphism presented no significant association in relation to CCL5 protein level. Considering the present findings, we suggest that CCL5 may be involved in BC staging and metastasis process.
KeywordsCCL5 ELISA Tumoral stages Breast cancer CCR5 Polymorphism
The authors would like to acknowledge the volunteers who made this study possible and the Londrina Cancer Hospital, Londrina, PR, Brazil. This study was suported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação Araucária, Secretaria da Ciência, Tecnologia e Ensino Superior (SETI), and Pró-Reitoria de Pesquisa e Pós-Graduação da Universidade Estadual de Londrina (PROPPG-UEL).
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Conflict of interest
The authors declare that they have no conflict of interests.
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