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Molecular Biology Reports

, Volume 46, Issue 4, pp 4483–4500 | Cite as

Positive impact of dynamic seeding of mesenchymal stem cells on bone-like biodegradable scaffolds with increased content of calcium phosphate nanoparticles

  • Pavla Sauerova
  • Tomas Suchy
  • Monika Supova
  • Martin Bartos
  • Jiri Klima
  • Jana Juhasova
  • Stefan Juhas
  • Tereza Kubikova
  • Zbynek Tonar
  • Radek Sedlacek
  • Marco Piola
  • Gianfranco Beniamino Fiore
  • Monica Soncini
  • Marie Hubalek KalbacovaEmail author
Original Article
  • 195 Downloads

Abstract

One of the main aims of bone tissue engineering, regenerative medicine and cell therapy is development of an optimal artificial environment (scaffold) that can trigger a favorable response within the host tissue, it is well colonized by resident cells of organism and ideally, it can be in vitro pre-colonized by cells of interest to intensify the process of tissue regeneration. The aim of this study was to develop an effective tool for regenerative medicine, which combines the optimal bone-like scaffold and colonization technique suitable for cell application. Accordingly, this study includes material (physical, chemical and structural) and in vitro biological evaluation of scaffolds prior to in vivo study. Thus, porosity, permeability or elasticity of two types of bone-like scaffolds differing in the ratio of collagen type I and natural calcium phosphate nanoparticles (bCaP) were determined, then analyzes of scaffold interaction with mesenchymal stem cells (MSCs) were performed. Simultaneously, dynamic seeding using a perfusion bioreactor followed by static cultivation was compared with standard static cultivation for the whole period of cultivation. In summary, cell colonization ability was estimated by determination of cell distribution within the scaffold (number, depth and homogeneity), matrix metalloproteinase activity and gene expression analysis of signaling molecules and differentiation markers. Results showed, the used dynamic colonization technique together with the newly-developed collagen-based scaffold with high content of bCaP to be an effective combined tool for producing bone grafts for bone implantology and regenerative medicine.

Keywords

Mesenchymal stem cells Collagen scaffolds Dynamic seeding Static cultivation Bone tissue engineering 

Notes

Acknowledgements

This study was supported by project 15-25813A awarded by the Ministry of Health of the Czech Republic and PROGRES Q26 provided by Charles University, Czech Republic. Special thanks go to Blanka Bilkova for her technical assistance.

Compliance with ethical standards

Conflict of interest

The authors declare no conflicts of interest.

Ethical approval

All experiments were carried out according to the guidelines for the care and use of experimental animals and approved by the Resort Professional Commission of the Czech Academy of Sciences for Approval of Projects of Experiments on Animals (Approved protocol No 32/2015 and 53/2015).

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Pavla Sauerova
    • 1
    • 2
  • Tomas Suchy
    • 4
    • 5
  • Monika Supova
    • 4
  • Martin Bartos
    • 1
    • 6
  • Jiri Klima
    • 7
  • Jana Juhasova
    • 7
  • Stefan Juhas
    • 7
  • Tereza Kubikova
    • 2
    • 3
  • Zbynek Tonar
    • 2
    • 3
  • Radek Sedlacek
    • 5
  • Marco Piola
    • 8
  • Gianfranco Beniamino Fiore
    • 8
  • Monica Soncini
    • 8
  • Marie Hubalek Kalbacova
    • 1
    • 2
    Email author
  1. 1.Institute of Pathological Physiology, 1st Faculty of MedicineCharles UniversityPragueCzech Republic
  2. 2.Biomedical Centre, Faculty of Medicine in PilsenCharles UniversityPilsenCzech Republic
  3. 3.Department of Histology and Embryology, Faculty of Medicine in PilsenCharles UniversityPlzeňCzech Republic
  4. 4.Department of Composites and Carbon Materials, Institute of Rock Structure and MechanicsAcademy of Sciences of the Czech RepublicPragueCzech Republic
  5. 5.Laboratory of Biomechanics, Department of Mechanics, Biomechanics and Mechatronics, Faculty of Mechanical EngineeringCzech Technical University in PraguePragueCzech Republic
  6. 6.Institute of Dental Medicine, 1st Faculty of MedicineCharles University and General University Hospital in PraguePragueCzech Republic
  7. 7.Laboratory of Cell Regeneration and Plasticity, Institute of Animal Physiology and GeneticsAcademy of Sciences of the Czech RepublicLibechovCzech Republic
  8. 8.Dipartimento di Elettronica, Informazione e BioingegneriaPolitecnico di MilanoMilanItaly

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