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Molecular Biology Reports

, Volume 46, Issue 4, pp 4085–4094 | Cite as

Myositis in Lewis rats induced by the superantigen Staphylococcal enterotoxin A

  • Alexander EmmerEmail author
  • Abimbola Abobarin-Adeagbo
  • Andreas Posa
  • Berit Jordan
  • Karl-Stefan Delank
  • Martin Sebastian Staege
  • Alexander Surov
  • Stephan Zierz
  • Malte Erich Kornhuber
Original Article
  • 95 Downloads

Abstract

The aetiology of inflammatory myopathies is not clearly known. A predominance of activated Cd8+ T lymphocytes in inflammatory infiltrates has already been detected. Superantigens activate lymphocytes in an oligoclonal manner. In the present investigation, we investigated local effects after injection of the superantigen (Sag) Staphylococcus enterotoxin A (SEA) in the quadriceps femoris muscle of Lewis rats. Histopathology and gene expression profiling was performed after injection of SEA or saline (control group) after one, three and 10 days. Histology revealed focal myositis predominated by Cd8+ T lymphocytes with a perimysial, endomysial and perivascular distribution, peaking 3 days after SEA injection. Using DNA microarray analysis (Affymetrix Rat Genome 230 2.0) genes that were differentially over-expressed at least 15 times at days one, three or ten after SEA injection were further analysed. One day after SEA injection over-expressed genes were related to the immune response (e.g. Fcnb, CD8a) but also to cell proliferation, differentiation and migration (e.g. Mpp2). Three days after SEA injection, differentially overexpressed genes were mainly related to the immune reaction with a clear signature for a Cd8+ T lymphocyte response (e.g. Cd3d, Cd8, Prf1, Gzmb). Ten days after SEA injection, the differentially overexpressed genes were again associated with the immune reaction (e.g. Cd3d, Il2) but also with regenerative processes and wound healing (e.g. Tgfa, Tpm1, Ripply1). The inflammatory response induced by SEA in Lewis rats shares histological and molecular similarities to polymyositis in humans. Therefore, SEA induced myositis can be taken as a new and apt model for polymyositis.

Keywords

Myositis Superantigen Staphylococcal enterotoxin A (SEA) Animal model CD8+ T-lymphocytes Microarray analysis 

Notes

Acknowledgements

We acknowledge the support by Novartis Pharma GmbH and by the research Grant (Grant No. FKZ 28/45) of the Wilhelm-Roux program of the Martin Luther University Halle-Wittenberg. We also acknowledge the excellent technical support by Angela Rosemeier.

Author contributions

AE and MEK designed the study AA, AP, BJ, AS, and MSS performed experiments, all authors analyzed the data and wrote the paper.

Compliance with ethical standards

Conflict of interest

The authors report no conflicts of interest in this work.

Supplementary material

11033_2019_4858_MOESM1_ESM.docx (39 kb)
Supplementary material 1 (DOCX 39 kb)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Alexander Emmer
    • 1
    Email author
  • Abimbola Abobarin-Adeagbo
    • 1
  • Andreas Posa
    • 1
  • Berit Jordan
    • 1
  • Karl-Stefan Delank
    • 2
  • Martin Sebastian Staege
    • 3
  • Alexander Surov
    • 4
  • Stephan Zierz
    • 1
  • Malte Erich Kornhuber
    • 1
  1. 1.Department of NeurologyMartin Luther University Halle-WittenbergHalle (Saale)Germany
  2. 2.Department of Orthopaedic SurgeryMartin Luther University Halle-WittenbergHalle (Saale)Germany
  3. 3.Department of Surgical and Conservative Paediatrics and Adolescent MedicineMartin Luther University Halle-WittenbergHalle (Saale)Germany
  4. 4.Department of Diagnostic and Interventional RadiologyUniversity of LeipzigLeipzigGermany

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