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Quantitative expression of microRNAs in Brassica oleracea infected with Xanthomonas campestris pv. campestris

  • Lucas Souza Santos
  • Mariana Rocha Maximiano
  • Esaú Megias
  • Marília Pappas
  • Simone Graça Ribeiro
  • Angela MehtaEmail author
Short Communication

Abstract

Brassica oleracea var. capitata (cabbage) is an economically important crop affected by black rot disease caused by Xanthomonas campestris pv. campestris (Xcc). MicroRNAs (miRNAs) play an important role in plant defense modulation and therefore the analysis of these molecules can help better understand plant-pathogen interactions. In this study, we report the differential expression of four miRNAs that seem to participate in the plant response to Xcc infection. Northern Blot and RT-qPCR techniques were used to measure miRNA expression in resistant (União) and susceptible (Kenzan) cultivars. From 6 miRNAs analyzed, 4 were detected and differentially expressed, showing a down- and upregulated expression profile in susceptible and resistant cultivars, respectively. These results suggest that miR156, miR167, miR169, and miR390 could play a role in B. oleracea resistance enhancement against Xcc and could be explored as potential resistance markers in B. oleracea-Xcc interaction.

Keywords

Black rot disease MicroRNAs RT-qPCR Northern blot Biomarkers 

Notes

Acknowledgements

This research was sponsored by Empresa Brasileira de Pesquisa Agropecuária (Embrapa), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Fundação de Apoio à Pesquisa do Distrito Federal (FAPDF).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Embrapa Recursos Genéticos e BiotecnologiaParque Estação Biológica, PqEBBrasíliaBrazil
  2. 2.Centro Universitário do Distrito FederalBrasíliaBrazil
  3. 3.Universidade Federal de Juiz de ForaJuiz de ForaBrazil
  4. 4.Universidad de CádizCádizSpain

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