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Synergistic effect of granzyme B-azurin fusion protein on breast cancer cells

  • Nafiseh Paydarnia
  • Shahryar Khoshtinat Nikkhoi
  • Azita Fakhravar
  • Mohsen Mehdiabdol
  • Hedieh Heydarzadeh
  • Saeed RanjbarEmail author
Original Article

Abstract

As one of the most prevalent malignancies, breast cancer still remains a significant risk for public health. Common therapeutic strategies include invasive surgery, chemotherapy and anti-herceptin antibodies. Adverse effects, drug resistance and low efficacy of current therapies necessitates the emergence of more effective platforms. Naturally released by the immune system, granzyme B activates multiple pro-apoptotic pathways by cleaving critical substrates. Bacterial cupredoxin, azurin, selectively targets cancer cells via a p53-dependent pathway. Fused by a linker, GrB-Azurin fusion protein was overexpressed in HEK293T cells, and purified by metal chromatography. SDS–PAGE, Western blotting and ELISA were performed to confirm successful expression, purification and analyze binding properties of the fusion protein. After treatment of various breast cancer cell lines with increasing concentrations of GrB-Azurin, quantitative real-time RT-PCR was used to measure relative expression of p21, Fas and DR5 pro-apoptotic genes. The results of DNA fragmentation and WST-1 cell viability assays indicated significant apoptosis induction in MDA-MB-231, MCF7 and SK-BR-3 cells, while insignificant cytotoxicity was detected on MCF 10A normal breast cells. Herein, we report the development of a novel biotherapeutic against breast cancer. Selective effectiveness of GrB-Azurin fusion protein on different breast cancer cells highlighted the potential of the designed construct as a candidate anti-cancer biodrug.

Keywords

Granzyme B Azurin Breast cancer Fusion protein Targeting 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interest regarding the publication of this article.

Supplementary material

11033_2019_4767_MOESM1_ESM.sbd (67 kb)
Supplementary material 1 (SBD 67 KB)
11033_2019_4767_MOESM2_ESM.sbd (67 kb)
Supplementary material 2 (SBD 66 KB)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Immunology Research CenterTabriz University of Medical SciencesTabrizIran
  2. 2.Department of Pharmaceutics, RutgersThe State University of New JerseyPiscatawayUSA
  3. 3.Department of Biology, Central Tehran BranchIslamic Azad UniversityTehranIran
  4. 4.Department of Medical Bacteriology, Faculty of Medical SciencesTarbiat Modares UniversityTehranIran
  5. 5.Department of Medical Biotechnology, Faculty of Medical SciencesTarbiat Modares UniversityTehranIran

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