Advertisement

Polyclonal predominance of concurrently producing OXA-23 and OXA-58 carbapenem-resistant Acinetobacter baumannii strains in a pediatric intensive care unit

  • Theodoros Karampatakis
  • Katerina Tsergouli
  • Lida Politi
  • Georgia Diamantopoulou
  • Elias Iosifidis
  • Charalampos Antachopoulos
  • Aggeliki Karyoti
  • Maria Sdougka
  • Athanassios Tsakris
  • Emmanuel RoilidesEmail author
Short Communication

Abstract

We report a predominance (64.7%) of polyclonal carbapenem-resistant Acinetobacter baumannii (CRAB) strains concurrently producing OXA-23 and OXA-58 carbapenemases in a pediatric intensive care unit in an endemic area. This is the first report of emergence of such double-OXA CRAB strains in a single unit worldwide.

Keywords

Acinetobacter baumannii Carbapenems Microbial resistance Pediatrics Critical care 

Notes

Acknowledgements

The authors would like to thank Vassiliki Pentsioglou for epidemiological data handling and her valuable collaboration during the study.

Funding

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.

References

  1. 1.
    Henig O, Weber G, Hoshen MB, Paul M, German L, Neuberger A, Gluzman I, Berlin A, Shapira C, Balicer RD (2015) Risk factors for and impact of carbapenem-resistant Acinetobacter baumannii colonization and infection: matched case-control study. Eur J Clin Microbiol Infect Dis 34(10):2063–2068.  https://doi.org/10.1007/s10096-015-2452-4 CrossRefGoogle Scholar
  2. 2.
    Russo A, Giuliano S, Ceccarelli G, Alessandri F, Giordano A, Brunetti G, Venditti M (2018) Comparison of septic shock due to multidrug-resistant Acinetobacter baumannii or Klebsiella pneumoniae carbapenemase-producing K. pneumoniae in intensive care unit patients. Antimicrob Agents Chemother.  https://doi.org/10.1128/AAC.02562-17 Google Scholar
  3. 3.
    Karampatakis T, Antachopoulos C, Tsakris A, Roilides E (2017) Molecular epidemiology of carbapenem-resistant Acinetobacter baumannii in Greece: an extended review (2000–2015). Future Microbiol 12:801–815.  https://doi.org/10.2217/fmb-2016-0200 CrossRefGoogle Scholar
  4. 4.
    Tsiatsiou O, Iosifidis E, Katragkou A, Dimou V, Sarafidis K, Karampatakis T, Antachopoulos C, Orfanou A, Tsakris A, Drossou-Agakidou V, Roilides E (2015) Successful management of an outbreak due to carbapenem-resistant Acinetobacter baumannii in a neonatal intensive care unit. Eur J Pediatr 174(1):65–74.  https://doi.org/10.1007/s00431-014-2365-8 CrossRefGoogle Scholar
  5. 5.
    Villalon P, Valdezate S, Medina-Pascual MJ, Carrasco G, Vindel A, Saez-Nieto JA (2013) Epidemiology of the Acinetobacter-derived cephalosporinase, carbapenem-hydrolysing oxacillinase and metallo-beta-lactamase genes, and of common insertion sequences, in epidemic clones of Acinetobacter baumannii from Spain. J Antimicrob Chemother 68(3):550–553.  https://doi.org/10.1093/jac/dks448 CrossRefGoogle Scholar
  6. 6.
    Karampatakis T, Geladari A, Politi L, Antachopoulos C, Iosifidis E, Tsiatsiou O, Karyoti A, Papanikolaou V, Tsakris A, Roilides E (2017) Cluster-distinguishing genotypic and phenotypic diversity of carbapenem-resistant Gram-negative bacteria in solid-organ transplantation patients: a comparative study. J Med Microbiol.  https://doi.org/10.1099/jmm.0.000541 Google Scholar
  7. 7.
    Tenover FC, Arbeit RD, Goering RV, Mickelsen PA, Murray BE, Persing DH, Swaminathan B (1995) Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 33(9):2233–2239Google Scholar
  8. 8.
    Pournaras S, Dafopoulou K, Del Franco M, Zarkotou O, Dimitroulia E, Protonotariou E, Poulou A, Zarrilli R, Tsakris A, Greek Study Group on Acinetobacter Antimicrobial R (2017) Predominance of international clone 2 OXA-23-producing-Acinetobacter baumannii clinical isolates in Greece, 2015: results of a nationwide study. Int J Antimicrob Agents 49(6):749–753.  https://doi.org/10.1016/j.ijantimicag.2017.01.028 CrossRefGoogle Scholar
  9. 9.
    Mathlouthi N, Ben Lamine Y, Somai R, Bouhalila-Besbes S, Bakour S, Rolain JM, Chouchani C (2018) Incidence of OXA-23 and OXA-58 carbapenemases coexpressed in clinical isolates of Acinetobacter baumannii in Tunisia. Microb Drug Resist 24(2):136–141.  https://doi.org/10.1089/mdr.2016.0306 CrossRefGoogle Scholar
  10. 10.
    D’Arezzo S, Principe L, Capone A, Petrosillo N, Petrucca A, Visca P (2011) Changing carbapenemase gene pattern in an epidemic multidrug-resistant Acinetobacter baumannii lineage causing multiple outbreaks in central Italy. J Antimicrob Chemother 66(1):54–61.  https://doi.org/10.1093/jac/dkq407 CrossRefGoogle Scholar
  11. 11.
    Zingg W, Holmes A, Dettenkofer M, Goetting T, Secci F, Clack L, Allegranzi B, Magiorakos AP, Pittet D, systematic review and evidence-based guidance on organization of hospital infection control programmes (SIGHT) study group (2015) Hospital organisation, management, and structure for prevention of health-care-associated infection: a systematic review and expert consensus. Lancet Infect Dis 15(2):212–224.  https://doi.org/10.1016/S1473-3099(14)70854-0 CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Theodoros Karampatakis
    • 1
    • 2
  • Katerina Tsergouli
    • 2
  • Lida Politi
    • 3
  • Georgia Diamantopoulou
    • 3
  • Elias Iosifidis
    • 1
    • 4
  • Charalampos Antachopoulos
    • 1
    • 4
  • Aggeliki Karyoti
    • 2
    • 4
  • Maria Sdougka
    • 5
  • Athanassios Tsakris
    • 3
  • Emmanuel Roilides
    • 1
    • 4
    Email author
  1. 1.Infectious Disease Unit, 3rd Department of Pediatrics, Medical FacultyAristotle University School of Health Sciences, Hippokration General HospitalThessalonikiGreece
  2. 2.Microbiology DepartmentHippokration General HospitalThessalonikiGreece
  3. 3.Microbiology DepartmentNational and Kapodistrian University School of MedicineAthensGreece
  4. 4.Infection Control CommitteeHippokration General HospitalThessalonikiGreece
  5. 5.Pediatric Intensive Care UnitHippokration General HospitalThessalonikiGreece

Personalised recommendations