Investigation of the phytochemical composition and antioxidant properties of chinar (Platanus orientalis L.) leaf infusion against ethanol-induced oxidative stress in rats

  • Abdulahad DoganEmail author
  • Ozgur Ozan Anuk
Original Article


Chinar (Platanus orientalis L.) is used in folk medicine against tooth and knee pain, wounds, inflammation, and stomach discomfort; however, the effects of P. orientalis leaf (PO-leaf) infusion on the liver and kidney are unknown. The aim of this study was to investigate the phytochemical composition and antioxidant properties of an infusion obtained from dried P. orientalis leaves against ethanol-induced oxidative stress (OS) in rats. After a toxicity test, thirty male Wistar rats were divided into five groups: Control, Ethanol 20%, Ethanol 20% + Silymarin (10 mg/kg), Ethanol 20% + PO-20 mg/mL infusion, and Ethanol 20% + PO-60 mg/mL infusion. The PO-leaf infusion doses were given ad libitum during 28 days to test the biochemical and antioxidant enzyme levels. According to the results, the PO-leaf contained rich compounds such as benzaldehyde, palmitic acid, 2,4-ditert-butylphenol, stearic acid, octadecanoic acid, linoleic acid, linolenic acid, kaempferol, and kaempferol derivatives. In the Ethanol group, AST, ALT, LDH, GGT, UA, and urea in the serum and GST and malondialdehyde (MDA) in the liver and erythrocyte tissues showed a significant increase compared to the Control group. AST, LDH, GGT, UA, and LDL-C levels in the serum and MDA (all tissues) significantly decreased in the Ethanol + PO-60 mg/mL group compared to the Ethanol group. SOD, GPx, and CAT activities in the kidney tissue of the Ethanol group showed a significant decrease compared to the Control group, whereas the GPx activity in kidney tissue in all of the treatment groups increased significantly compared to the Ethanol group. These findings suggest that the administration of the determined PO-leaf infusion doses might have a protective role against ethanol-induced liver and kidney damage in rats.

Graphical abstract


Chinar (Platanus orientalis L.) Ethanol Silymarin Antioxidant enzymes and oxidative stress Bioactive compounds Rat 



Antioxidant defence systems


Alkalane phosphatase


Alanine aminotransferase


Aspartate aminotransferase








Gas chromatography mass spectrometry


Gama glutamyl transferase




Glutathione peroxidase


Glutathione reductase


Reduced glutathine


Glutathione S-transferase


High density lipoprotein-cholesterol


High performance liquid chromatography


Lactate dehydrogenase


Low density lipoprotein-cholesterol




Oxidative stress

PO-leaf infusion

Platanus orientalis leaf infusion


Reactive oxygen species


Superoxide dismutase


Total bilirubin


Total triglyceride


Uric acid



This work was financially supported by the Van Yuzuncu Yil University Scientific Research Project Commission (Grant No: TYL-2018-6774). The authors are grateful to Dr. Abdullah Dalar for his invaluable contributions to the determination of the active substance, and to Van Yuzuncu Yil University for providing financial assistance for this study. A. Dogan was the main moderator of the study. O.O. Anuk performed the biochemical investigation and treatment in this study.

Compliance with ethical standards

Conflict of interest

On behalf of the authors, the corresponding author states that there are no conflicts of interest.

Research involving human and animal participants

Rodents were used in this study. Male Wistar albino rats of approximately 2 months of age and an average weight of 200 g were provided by the Experimental Animal Research Center, Van Yuzuncu Yil University (Van, Turkey). They were divided into five groups, with each group containing six rats. The animals were housed at 25 ± 2 °C at a light/dark photoperiod of 10:14. All of animals were given a wheat-soybean-based diet and water ad libitum in stainless steel cages, and received humane care according to the criteria outlined in the ‘Guide for the Care and Use of Laboratory Animals’ prepared by the National Academy of Science and published by the National Institute of Health. The ethic regulations followed were in accordance with national and institutional guidelines for the protection of animal welfare during experiments. This study was approved by the Ethic Committee of Van Yuzuncu Yil University (Protocol number: 27552122-604.01.02-E.70881). No human participants were involved in the present research.


  1. 1.
    Khan AS (2017) Woody Plants with Possible Anti-HIV Activity. In: Khan AS (ed) Medicinally important trees. Springer International Publishing, Switzerland, pp 109–131Google Scholar
  2. 2.
    Carpenter RJ, Hill RS, Jordan GJ (2005) Leaf cuticular morphology links Platanaceae and Proteaceae. Int J Plant Sci 166(5):843–855Google Scholar
  3. 3.
    Haider S, Nazreen S, Alam MM et al (2012) Anti-inflammatory and anti-nociceptive activities of Platanus orientalis Linn. and its ulcerogenic risk evaluation. J Ethnopharmacol 143(1):236–240Google Scholar
  4. 4.
    Khosropour E, Attarod P, Shirvany A et al (2017) Response of Platanus orientalis leaves to urban pollution by heavy metals. J For Res 1–9Google Scholar
  5. 5.
    Janković B, Dodevski V, Stojmenović M et al. (2018) Characterization analysis of raw and pyrolyzed plane tree seed (Platanus orientalis L.) samples for its application in carbon capture and storage (CCS) technology. J Therm Anal Calorim 133(1):465–480Google Scholar
  6. 6.
    Mitrokotsa D, Mitaku S, Demetzos C et al. (1993) Bioactive compounds from the buds of Platanus orientalis and isolation of a new kaempferol glycoside. Planta Med 59(06):517–520Google Scholar
  7. 7.
    Dimas K, Demetzos C, Mitaku S et al. (2000) Cytotoxic activity of kaempferol glycosides against human leukaemic cell lines in vitro. Pharmacol Res 41(1):83–86Google Scholar
  8. 8.
    El-Alfy TS, El-Gohary HMA, Sokkar NM et al. (2008) Two novel acylated flavonol glycosides from Platanus orientalis L. leaves. Nat Prod Commun 3:1899–1902Google Scholar
  9. 9.
    Tantry MA, Akbar S, Dar JA et al. (2012) Acylated flavonol glycoside from Platanus orientalis. Fitoterapia 83(2):281–285Google Scholar
  10. 10.
    Nishanbaev SZ, Kuliev ZA, Khidyrova NK et al. (2010) New oligomeric proanthocyanidin glycosides Platanoside A and Platanoside B from Platanus orientalis trunk bark. Chem Nat Compd 46:357–362Google Scholar
  11. 11.
    Khidyrova NK, Rashkes YV, Rashkes AM et al. (1995) Shed plane leaves as a source of α-tocopherol. Chem Nat Compd 31:312–314Google Scholar
  12. 12.
    Abdullaev UA, Rashkes YV, Khidyrova NK et al. (1994) Mass-spectrometric analysis of phytol derivatives from the leaves of Platanus orientalis. Chem Nat Compd 30(3):332–338Google Scholar
  13. 13.
    Bastos DZ, Pimentel IC, de Jesus DA et al. (2007) Biotransformation of betulinic and betulonic acids by fungi. Phytochemistry 68(6):834–839Google Scholar
  14. 14.
    Asadbeigi M, Mohammadi T, Rafieian-Kopaei M et al. (2014) Traditional effects of medicinal plants in the treatment of respiratory diseases and disorders: an ethnobotanical study in the Urmia. Asian Pac J Trop Med 7:364–368Google Scholar
  15. 15.
    Shende S, Joshi KA, Kulkarni AS et al (2018) Platanus orientalis Leaf Mediated Rapid Synthesis of Catalytic Gold and Silver Nanoparticles. J Nanomed Nanotechnol 9(494):2Google Scholar
  16. 16.
    Cederbaum AI, Lu Y, Wu D (2009) Role of oxidative stress in alcohol-induced liver injury. Arch Toxicol 83(6):519–548Google Scholar
  17. 17.
    Gülçin I (2012) Antioxidant activity of food constituents: an overview. Arch toxicol 86(3):345–391Google Scholar
  18. 18.
    Dogan A, Dalar A, Sadullahoglu C et al (2018) Investigation of the protective effects of horse mushroom (Agaricus arvensis Schaeff.) against carbon tetrachloride-induced oxidative stress in rats. Mol Biol Rep 45:787–797Google Scholar
  19. 19.
    Nwozo SO, Ajagbe AA, Oyinloye BE (2012) Hepatoprotective effect of Piper guineense aqueous extract against ethanol-induced toxicity in male rats. J Exp Integr Med 2(1):71–76Google Scholar
  20. 20.
    Ntchapda F, Abakar D, Kom B et al (2014) Acute and sub-chronic oral toxicity assessment of the aqueous extract leaves of Ficus glumosa Del. (Moraceae) in rodents. J Intercult Ethnopharmacol 3(4):206–213Google Scholar
  21. 21.
    Dogan A, Celik I, Kaya MS (2015) Antidiabetic properties of lyophilized extract of acorn (Quercus brantii Lindl.) on experimentally STZ-induced diabetic rats. J ethnopharmacol 176:243–251Google Scholar
  22. 22.
    Nath P, Yadav AK (2015) Acute and sub-acute oral toxicity assessment of the methanolic extract from leaves of Hibiscus rosa-sinensis L. in mice. J Intercult 4(1):70–73Google Scholar
  23. 23.
    Wu T, Tang Q, Yu Z et al (2014) Inhibitory effects of sweet cherry anthocyanins on the obesity development in C57BL/6 mice. Int J Food Sci Nutr 65(3):351–359Google Scholar
  24. 24.
    Wu T, Qi X, Liu Y et al (2013) Dietary supplementation with purified mulberry (Morus australis Poir) anthocyanins suppresses body weight gain in high-fat diet fed C57BL/6 mice. Food Chem 141(1):482–487Google Scholar
  25. 25.
    Abenavoli L, Capasso R, Milic N et al (2010) Milk thistle in liver diseases: past, present, future. Phytother Res 24:1423–1432Google Scholar
  26. 26.
    Ding TM, Tian SJ, Zhang ZX et al (2001) Determination of active component in silymarin by RP-LC and LC/MS. J Pharmacol Biomed Anal 26(1):155–161Google Scholar
  27. 27.
    Kocaman N, DÖ D (2015) Hepatoprotektif bir ajan: silymarin. Firat Med J 20(3):128–132Google Scholar
  28. 28.
    Yayalacı Y, Celik I, Batı B (2014) Hepatoprotective and antioxidant activity of linden (Tilia platyphyllos L.) infusion against ethanol-induced oxidative stress in rats. J Membr Boil 247(2):181–188Google Scholar
  29. 29.
    Dogan A, Celik I (2012) Hepatoprotective and antioxidant activities of grapeseeds against ethanol-induced oxidative stress in rats. Br J Nutr 107(1):45–51Google Scholar
  30. 30.
    Mannervik B, Guthenberg C (1981) Glutathione S-transferase (human placenta). Methods Enzymol 77:231–235Google Scholar
  31. 31.
    Paglia DE, Valentine WN (1967) Studies on quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70:158–169Google Scholar
  32. 32.
    McCord JM, Fridovich I (1969) Superoxide dismutase, an enzymatic function for erythrocuprein (hemocuprein). J Biol Chem 244:6049–6053Google Scholar
  33. 33.
    Aebi H (1974) Catalase. In: Bergmeyer HU (ed) Methods of enzymatic analysis. Academic Press, New York-London, pp 673–684Google Scholar
  34. 34.
    Jain SK, McVie R, Duett J et al (1989) Erythrocyte membrane lipid peroxidation and glycolylated hemoglobin in diabetes. Diabetes 38:1539–1543Google Scholar
  35. 35.
    Beutler E, Dubon OB, Kelly M (1963) Improved method for the determination of blood glutathione. J Lab Clin Med 61:882–888Google Scholar
  36. 36.
    Ebn-e-Sina A (1988) Ghanoon Dar Teb, vol 2. Soroosh Press, Tehran, pp 119–120Google Scholar
  37. 37.
    Azevedo LF, da Silva SM, Navarro LB et al (2016) Evidence of anti-inflammatory and antinociceptive activities of Plinia edulis leaf infusion. J ethnopharmacol 192:178–182Google Scholar
  38. 38.
    Oyeleke SA, Ajayi AM, Umukoro S et al (2018) Anti-inflammatory activity of Theobroma cacao L. stem bark ethanol extract and its fractions in experimental models. J ethnopharmacol 222:239–248Google Scholar
  39. 39.
    Pari L, Suresh A (2008) Effect of grape (Vitis vinifera L.) leaf extract on alcohol induced oxidative stress in rats. Food Chem Toxicol 46(5):1627–1634Google Scholar
  40. 40.
    Halliwell B, Gutteridge JM (2015) Free radicals in biology and medicine. Oxford University Press, USAGoogle Scholar
  41. 41.
    Dalar A, Dogan A, Bengu AS et al (2018) Screening in vivo antioxidant and haematological properties of sumac and acorn bioactive rich extracts. Ind Crops Prod 124:20–27Google Scholar
  42. 42.
    Zakaria NNA, Okello EJ, Howes MJ et al (2018) In vitro protective effects of an aqueous extract of Clitoria ternatea L. flower against hydrogen peroxide-induced cytotoxicity and UV-induced mtDNA damage in human keratinocytes. Phytother Res 32(6):1064–1072Google Scholar
  43. 43.
    Chang CJ, Tzeng TF, Liou SS et al (2011) Kaempferol regulates the lipid-profile in high-fat diet-fed rats through an increase in hepatic PPARα levels. Planta med 77(17):1876–1882Google Scholar
  44. 44.
    Zang Y, Zhang L, Igarashi K et al (2015) The anti-obesity and anti-diabetic effects of kaempferol glycosides from unripe soybean leaves in high-fat-diet mice. Food Func 6(3):834–841Google Scholar
  45. 45.
    Abliz A, Aji Q, Abdusalam E et al (2014) Effect of Cydonia oblonga Mill. leaf extract on serum lipids and liver function in a rat model of hyperlipidaemia. J Ethnopharmacol 151(2):970–974Google Scholar
  46. 46.
    Abarikwu SO, Njoku RC, Lawrence CJ et al (2017) Rutin ameliorates oxidative stress and preserves hepatic and renal functions following exposure to cadmium and ethanol. Pharm Biol 55(1):2161–2169Google Scholar
  47. 47.
    Choi SH, Lee AY, Park CH et al (2018) Protective effect of Carthamus tinctorius L. seed on oxidative stress and cognitive impairment induced by chronic alcohol consumption in mice. Food Sci Biotechnol 27(5):1475–1484Google Scholar
  48. 48.
    Zhang H, Ma Z, Luo X et al (2018) Effects of mulberry fruit (Morus alba L.) consumption on health outcomes: a mini-review. Antioxidants 7(5):69Google Scholar
  49. 49.
    Bati B, Celik I, Dogan A (2015) Determination of hepatoprotective and antioxidant role of walnuts against ethanol-induced oxidative stress in rats. Cell Biochem Biophys 71(2):1191–1198Google Scholar
  50. 50.
    Turan A, Celik I (2016) Antioxidant and hepatoprotective properties of dried fig against oxidative stress and hepatotoxicity in rats. Int J Biol Macromol 91:554–559Google Scholar
  51. 51.
    Das M, Basu S, Banerjee B et al (2018) Hepatoprotective effects of green Capsicum annum against ethanol induced oxidative stress, inflammation and apoptosis in rats. J Ethnopharmacol 227:69–81Google Scholar
  52. 52.
    Cao YW, Jiang Y, Zhang DY et al (2015) Protective effects of Penthorum chinense Pursh against chronic ethanol-induced liver injury in mice. J Ethnopharmacol 161:92–98Google Scholar
  53. 53.
    Elkomy NM, Ibrahim IAH, Elshazly SM et al (2018) Ameliorative effects of clonidine on ethanol induced kidney injury in rats: Potential role for imidazoline-1 receptor. Eur J Pharmacol 824:148–156Google Scholar
  54. 54.
    Chen X, Ying X, Sun W et al (2018) The therapeutic effect of fraxetin on ethanol-induced hepatic fibrosis by enhancing ethanol metabolism, inhibiting oxidative stress and modulating inflammatory mediators in rats. Int Immunopharmacol 56:98–104Google Scholar
  55. 55.
    Hamid A, Ibrahim FW, Ming TH et al (2018) Zingiber zerumbet L.(Smith) extract alleviates the ethanol-induced brain damage via its antioxidant activity. BMC Complement Altern Med 18(1):101Google Scholar
  56. 56.
    Panigrahi GK, Yadav A, Yadav A et al (2014) Hepatic transcriptional analysis in rats treated with Cassia occidentalis seed: Involvement of oxidative stress and impairment in xenobiotic metabolism as a putative mechanism of toxicity. Toxicol Lett 229(1):273–283Google Scholar
  57. 57.
    Pan PH, Lin SY, Ou YC et al (2010) Stearic acid attenuates cholestasis-induced liver injury. Biochem Biophys Res Commun 391(3):1537–1542Google Scholar
  58. 58.
    Vieira AED, Araujo GL, Galassi CM et al (2013) Toxicological, toxicokinetic and gastroprotective evaluation of the benzaldehyde semicarbazone. Food Chem Toxicol 55:434–443Google Scholar
  59. 59.
    Langeswaran K, Selvaraj J, Ponnulakshmi R et al (2018) Protective Effect of Kaempferol on Biochemical and Histopathological Changes in Mercuric Chloride Induced Nephrotoxicity in Experimental Rats. J Biol Act Prod Nat 8(2):125–136Google Scholar
  60. 60.
    Mota NS, Kviecinski MR, Zeferino RC et al (2018) In vivo antitumor activity of by-products of Passiflora edulis f. flavicarpa Deg. Rich in medium and long chain fatty acids evaluated through oxidative stress markers, cell cycle arrest and apoptosis induction. Food ChemToxicol 118:557–565Google Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Biochemistry, Faculty of PharmacyVan Yuzuncu Yil UniversityVanTurkey

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