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Investigation of the phytochemical composition and antioxidant properties of chinar (Platanus orientalis L.) leaf infusion against ethanol-induced oxidative stress in rats

  • Abdulahad DoganEmail author
  • Ozgur Ozan Anuk
Original Article
  • 37 Downloads

Abstract

Chinar (Platanus orientalis L.) is used in folk medicine against tooth and knee pain, wounds, inflammation, and stomach discomfort; however, the effects of P. orientalis leaf (PO-leaf) infusion on the liver and kidney are unknown. The aim of this study was to investigate the phytochemical composition and antioxidant properties of an infusion obtained from dried P. orientalis leaves against ethanol-induced oxidative stress (OS) in rats. After a toxicity test, thirty male Wistar rats were divided into five groups: Control, Ethanol 20%, Ethanol 20% + Silymarin (10 mg/kg), Ethanol 20% + PO-20 mg/mL infusion, and Ethanol 20% + PO-60 mg/mL infusion. The PO-leaf infusion doses were given ad libitum during 28 days to test the biochemical and antioxidant enzyme levels. According to the results, the PO-leaf contained rich compounds such as benzaldehyde, palmitic acid, 2,4-ditert-butylphenol, stearic acid, octadecanoic acid, linoleic acid, linolenic acid, kaempferol, and kaempferol derivatives. In the Ethanol group, AST, ALT, LDH, GGT, UA, and urea in the serum and GST and malondialdehyde (MDA) in the liver and erythrocyte tissues showed a significant increase compared to the Control group. AST, LDH, GGT, UA, and LDL-C levels in the serum and MDA (all tissues) significantly decreased in the Ethanol + PO-60 mg/mL group compared to the Ethanol group. SOD, GPx, and CAT activities in the kidney tissue of the Ethanol group showed a significant decrease compared to the Control group, whereas the GPx activity in kidney tissue in all of the treatment groups increased significantly compared to the Ethanol group. These findings suggest that the administration of the determined PO-leaf infusion doses might have a protective role against ethanol-induced liver and kidney damage in rats.

Graphical abstract

Keywords

Chinar (Platanus orientalis L.) Ethanol Silymarin Antioxidant enzymes and oxidative stress Bioactive compounds Rat 

Abbreviations

ADS

Antioxidant defence systems

ALP

Alkalane phosphatase

ALT

Alanine aminotransferase

AST

Aspartate aminotransferase

CAT

Catalase

CHOL

Cholesterol

CREA

Creatinine

GC-MS

Gas chromatography mass spectrometry

GGT

Gama glutamyl transferase

Glu

Glucose

GPx

Glutathione peroxidase

GR

Glutathione reductase

GSH

Reduced glutathine

GST

Glutathione S-transferase

HDL-C

High density lipoprotein-cholesterol

HPLC

High performance liquid chromatography

LDH

Lactate dehydrogenase

LDL-C

Low density lipoprotein-cholesterol

MDA

Malondialdehyde

OS

Oxidative stress

PO-leaf infusion

Platanus orientalis leaf infusion

ROS

Reactive oxygen species

SOD

Superoxide dismutase

TB

Total bilirubin

TRIG

Total triglyceride

UA

Uric acid

Notes

Acknowledgements

This work was financially supported by the Van Yuzuncu Yil University Scientific Research Project Commission (Grant No: TYL-2018-6774). The authors are grateful to Dr. Abdullah Dalar for his invaluable contributions to the determination of the active substance, and to Van Yuzuncu Yil University for providing financial assistance for this study. A. Dogan was the main moderator of the study. O.O. Anuk performed the biochemical investigation and treatment in this study.

Compliance with ethical standards

Conflict of interest

On behalf of the authors, the corresponding author states that there are no conflicts of interest.

Research involving human and animal participants

Rodents were used in this study. Male Wistar albino rats of approximately 2 months of age and an average weight of 200 g were provided by the Experimental Animal Research Center, Van Yuzuncu Yil University (Van, Turkey). They were divided into five groups, with each group containing six rats. The animals were housed at 25 ± 2 °C at a light/dark photoperiod of 10:14. All of animals were given a wheat-soybean-based diet and water ad libitum in stainless steel cages, and received humane care according to the criteria outlined in the ‘Guide for the Care and Use of Laboratory Animals’ prepared by the National Academy of Science and published by the National Institute of Health. The ethic regulations followed were in accordance with national and institutional guidelines for the protection of animal welfare during experiments. This study was approved by the Ethic Committee of Van Yuzuncu Yil University (Protocol number: 27552122-604.01.02-E.70881). No human participants were involved in the present research.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Department of Biochemistry, Faculty of PharmacyVan Yuzuncu Yil UniversityVanTurkey

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