Fractionated whole body gamma irradiation modulates the hepatic response in type II diabetes of high fat diet model rats
HFD animals were exposed to a low rate of different fractionated whole body gamma irradiation doses (0.5, 1 and 2 Gy, three fractions per week for two consecutive months) and the expression of certain genes involved in type 2 diabetes mellitus (T2DM) in livers and brains of HFD Wistar rats was investigated. Additionally, levels of diabetes-related proteins encoded by the studied genes were analyzed. Results indicated that mRNA level of incretin glucagon like peptite-1 receptor (GLP-1R) was augmented in livers and brains exposed to 1 and 2 Gy doses. Moreover, the mitochondrial uncoupling proteins 2 and 3 (UCP2/3) expressions in animals fed on HFD compared to those fed on normal chow diet were significantly increased at all applied doses. GLP-1R and UCP3 protein levels were up regulated in livers. Total protein content increased at 0.5 and 1 Gy gamma irradiation exposure and returned to its normal level at 2 Gy dose. Results could be an indicator of type 2 diabetes delayed development during irradiation exposure and support the importance of GLP-1R as a target gene in radiotherapy against T2DM and its chronic complications. A new hypothesis of brain-liver and intestine interface is speculated by which an increase in the hepatic GLP-1R is influenced by the effect of fractionated whole body gamma irradiation.
KeywordsFractionated whole body gamma irradiation (FWBGI) Liver Type 2 diabetes GLP-1 GLP-1R HFD
The authors wish to express their deep appreciation to Prof. Ibrahim Othman, the director general of the Atomic Energy Commission of Syria (AECS). Thanks are also extended to Dr. M. A. Bakir, the head of the radiation medicine department for his help and support throughout the period of this research and to Dr. K. Aljoumaa for his appreciated scientific advices.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no competing interests.
All institutional and national guidelines for the care and use of laboratory animals were obeyed by the Local Scientific and Ethical Committee of the Atomic Energy Commission of Syria (AECS), Damascus, Syria (permit number is 2-28/10/2018). This article does not contain any studies with human participants performed by any of the authors.
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