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Genome-wide identification and characterization of Hsp70 gene family in Nicotiana tabacum

  • Zhaopeng Song
  • Feilong Pan
  • Xiaoping Lou
  • Daibin Wang
  • Chao Yang
  • Baoquan Zhang
  • Hongying ZhangEmail author
Original Article
  • 25 Downloads

Abstract

Heat shock proteins 70 (Hsp70) constitute a highly conserved protein family of cellular chaperones widely distributed in plants, where they play a fundamental role in response to biotic and abiotic stress. Until now, genome-wide analyses of the Hsp70 gene family have been conducted for some species. However, reports about Hsp70 genes in Nicotiana tabacum are scarce. In this study, we systematically conducted genome-wide identification and expression analysis of the Hsp70 gene family in tobacco, including gene structure, classification, evolutionary relationships, promoters, and transcript levels in response to abiotic stress treatments. In all, 61 Hsp70 members were identified and classified into six groups that were mapped onto 18 chromosomes, where most were distributed on both ends of the chromosome. The conserved structures and motifs of NtHsp70 proteins in the same subfamily were highly consistent. At least 15 pairs of NtHsp70 genes underwent gene duplication by segment and tandem duplications. Most NtHsp70 proteins contained N-terminal hexokinase conserved motifs. Phylogenetic analysis showed that most species expanded according to their own species-specific approach during the evolution of Hsp70s. Tissue-specific expression analysis indicated that all NtHsp70 genes were involved in at least one or more abiotic stress responses, highlighting the wide participation of NtHsp70 genes in environmental adaptation. This is the first genome-wide analysis of Hsp70 in N. tabacum. These results indicate that each NtHsp70 member fulfilled distinct functions in response to various abiotic stresses.

Keywords

Nicotiana tabacum Heat shock protein 70 Genome-wide analysis Synteny analysis Phylogenetic analysis Expression profile analysis 

Abbreviations

Hsp70

Heat shock protein 70

NtHsp70

Gene in Nicotiana tabacum

ABA

Abscisic acid

PEG

Polyethylene glycol

qRT-PCR

Quantitative real-time PCR

Chlo

Chloroplast

Cyto

Cytosol

Endo

Endoplasmic reticulum

Mito

Mitochondria

Notes

Acknowledgements

We thank China National Tobacco Corp., Chongqing Division and Henan Agricultural University for financial support and technical assistance. We also appreciate the reviewers and editors for the patience to the work.

Authors’ contributions

ZPS and FLP conceived and designed the experiments. XPL and DBW performed the experiments and participated to the data analysis. CY and BQZ performed the qRT-PCR experiments. HYZ revised the manuscript.

Funding

This work was supported by China National Tobacco Corp., Yunnan Division (2016YN10, 2017YN19) and China National Tobacco Corp., Chongqing Division (NY20180601070002).

Compliance with ethical standards

Conflicts of interest

The authors declare that they have no conflicts of interest.

Supplementary material

11033_2019_4644_MOESM1_ESM.docx (3.3 mb)
Supplementary material 1 (DOCX 3388 KB)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Key Laboratory for Cultivation of Tobacco Industry, College of Tobacco ScienceHenan Agricultural UniversityZhengzhouChina
  2. 2.China Tobacco Zhejiang Industrial Co., LtdHangzhouChina
  3. 3.Chongqing Tobacco Science Research InstituteChongqingChina

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