Genome-wide identification and characterization of Hsp70 gene family in Nicotiana tabacum
- 25 Downloads
Heat shock proteins 70 (Hsp70) constitute a highly conserved protein family of cellular chaperones widely distributed in plants, where they play a fundamental role in response to biotic and abiotic stress. Until now, genome-wide analyses of the Hsp70 gene family have been conducted for some species. However, reports about Hsp70 genes in Nicotiana tabacum are scarce. In this study, we systematically conducted genome-wide identification and expression analysis of the Hsp70 gene family in tobacco, including gene structure, classification, evolutionary relationships, promoters, and transcript levels in response to abiotic stress treatments. In all, 61 Hsp70 members were identified and classified into six groups that were mapped onto 18 chromosomes, where most were distributed on both ends of the chromosome. The conserved structures and motifs of NtHsp70 proteins in the same subfamily were highly consistent. At least 15 pairs of NtHsp70 genes underwent gene duplication by segment and tandem duplications. Most NtHsp70 proteins contained N-terminal hexokinase conserved motifs. Phylogenetic analysis showed that most species expanded according to their own species-specific approach during the evolution of Hsp70s. Tissue-specific expression analysis indicated that all NtHsp70 genes were involved in at least one or more abiotic stress responses, highlighting the wide participation of NtHsp70 genes in environmental adaptation. This is the first genome-wide analysis of Hsp70 in N. tabacum. These results indicate that each NtHsp70 member fulfilled distinct functions in response to various abiotic stresses.
KeywordsNicotiana tabacum Heat shock protein 70 Genome-wide analysis Synteny analysis Phylogenetic analysis Expression profile analysis
Heat shock protein 70
Gene in Nicotiana tabacum
Quantitative real-time PCR
We thank China National Tobacco Corp., Chongqing Division and Henan Agricultural University for financial support and technical assistance. We also appreciate the reviewers and editors for the patience to the work.
ZPS and FLP conceived and designed the experiments. XPL and DBW performed the experiments and participated to the data analysis. CY and BQZ performed the qRT-PCR experiments. HYZ revised the manuscript.
This work was supported by China National Tobacco Corp., Yunnan Division (2016YN10, 2017YN19) and China National Tobacco Corp., Chongqing Division (NY20180601070002).
Compliance with ethical standards
Conflicts of interest
The authors declare that they have no conflicts of interest.
- 7.Eckl JM, Richter K (2013) Functions of the Hsp90 chaperone system: lifting client proteins to new heights. Int J Biochem Mol Biol 4:157Google Scholar
- 16.Zhang Y, Wang M, Chen J, Rong J, Ding M (2014) Genome-wide analysis of HSP70 superfamily in Gossypium raimondii and the expression of orthologs in Gossypium hirsutum. Hereditas 36:921–933Google Scholar
- 21.Cuimin L, Felix W, Golecki JR, Sabrina C, Barbara H, Christine M, Michael S (2010) The chloroplast HSP70B-CDJ2-CGE1 chaperones catalyse assembly and disassembly of VIPP1 oligomers in Chlamydomonas. Plant J Cell Mol Biol 50:265–277Google Scholar
- 37.Bing L, Liu H, Sun D, Zhou R (2002) Signal transduction mechanism of plant heat shock response. Acta Photophysiol Sin 28:1–10Google Scholar
- 43.Anderson JV, Guy CL (1995) Spinach leaf 70-kilodalton heat-shock cognate stabilizes bovine adrenal glucose-6-phosphate dehydrogenase in vitro without apparent stable binding. Planta 196:303–310Google Scholar
- 47.Fujita Y, Fujita M, Satoh R, Maruyama K, Parvez MM, Seki M, Hiratsu K, Ohme-Takagi M, Shinozaki K, Yamaguchi-Shinozaki K (2005) AREB1 is a transcription activator of novel ABRE-dependent ABA signaling that enhances drought stress tolerance in Arabidopsis. Plant Cell 17:3470–3488CrossRefGoogle Scholar