In present study, forensic parameters based on the population data of nineteen autosomal short tandem repeats (STRs) loci were estimated in Chinese Han populations from three riverfront areas of Yangtze Basin in Eastern China. The combined power of discrimination (CPD) were 0.9999999999999999999999883, 0.9999999999999999999999846 and 0.9999999999999999999999863, respectively. The combined power of exclusion (CPE) were 0.999999992248957, 0.999999989800400 and 0.999999989799819, respectively. Furthermore, the genetic relationships among our studied populations and 32 previously investigated populations residing in other areas of China were also analyzed based on pairwise genetic distances (FST). The multidimensional scaling (MDS) plot and phylogenetic tree indicated that our studied populations were majorly clustered with the Han populations along the Yangtze Basin and populations to the north of it. In summary, our results supported that the 19 autosomal STR loci, which were polymorphic and informative in forensic identification, could also be applied in population genetics studies.
Forensic genetics Short tandem repeat Yangtze Basin Eastern Chinese Han Phylogenetic relationship
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This study was supported by grants from the National Key R&D Program of China (Grant No. 2016YFC0800703), Standard Program of Shanghai Municipality (Grant Nos. 16DZ0501600, 18DZ1200300), Public Interest Research Grant Programs of National Research Institutes (Grant No. GY2017D-2) and the National Natural Science Foundation (Grant Nos. 81625013, 81772028). The funders had no role in study design, data analysis, publishing decisions, or manuscript preparation.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
Fig. S1. A heatmap illustrating the allele distribution of our studied populations and other 22 previously reported Han populations. The color gradually turns from white to red as the allelic frequency increases. Hierarchical clustering based on the Euclidean distances was also performed on each shared locus—Supplementary material 2 (TIF 28658 KB)
Fig. S2. A map showing the geographical distribution of our studied populations and another 32 previously published Chinese populations applied in this comparison, including 22 Han populations and 10 ethnic minorities. The three studied populations were underlined—Supplementary material 3 (TIF 10113 KB)
Jobling MA, Gill P (2004) Encoded evidence: DNA in forensic analysis. Nat Rev Genet 5(10):739–751CrossRefGoogle Scholar
Kayser M, de Knijff P (2011) Improving human forensics through advances in genetics, genomics and molecular biology. Nat Rev Genet 12(3):179–192CrossRefGoogle Scholar
Walsh PS, Metzger DA, Higuchi R (2013) Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 54(3):134–139CrossRefGoogle Scholar
Excoffier L, Lischer HE (2010) Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Resour 10(3):564–567CrossRefGoogle Scholar
Zhao F, Wu X, Cai G, Xu C (2003) The application of Modified-Powerstates software in forensic biostatistics (in Chinese). Chin J Forensic Med 18(5):297–298Google Scholar
Hansen J (2000) Using SPSS for windows and Macintosh: analyzing and understanding data. Am Stat 59(1):113CrossRefGoogle Scholar
Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33(7):1870–1874CrossRefGoogle Scholar
Yuan J, Wang X, Shen C, Liu W, Yan J, Wang H et al (2014) Genetic profile characterization and population study of 21 autosomal STR in Chinese Kazak ethnic minority group. Electrophoresis 35(4):503–510CrossRefGoogle Scholar
Sokal RR, Michener CD (1958) A statistical method for evaluating systematic relationships. Univ Kansas Sci Bull 38:1409–1437Google Scholar
Xu S, Yin X, Li S, Jin W, Lou H, Yang L et al (2009) Genomic dissection of population substructure of Han Chinese and its implication in association studies. Am J Hum Genet 85(6):762–774CrossRefGoogle Scholar
He G, Wang Z, Wang M, Hou Y (2017) Genetic diversity and phylogenetic differentiation of southwestern Chinese Han: a comprehensive and comparative analysis on 21 non-CODIS STRs. Sci Rep 7(1):13730CrossRefGoogle Scholar
Zou X, Li Y, Li P, Nie Q, Wang T, Hu Y et al (2017) Genetic polymorphisms for 19 autosomal STR loci of Chongqing Han ethnicity and phylogenetic structure exploration among 28 Chinese populations. Int J Legal Med 131(6):1539–1542CrossRefGoogle Scholar
Wang M, Wang Z, Zhang Y, He G, Liu J, Hou Y (2017) Forensic characteristics and phylogenetic analysis of two Han populations from the southern coastal regions of China using 27 Y-STR loci. Forensic Sci. Int Genet 31:e17–e23CrossRefGoogle Scholar
Nothnagel M, Fan G, Guo F, He Y, Hou Y, Hu S et al (2017) Revisiting the male genetic landscape of China: a multi-center study of almost 38,000 Y–STR haplotypes. Hum Genet 136(5):485–497CrossRefGoogle Scholar
Zhang S, Bian Y, Li L, Sun K, Wang Z, Zhao Q et al (2015) Population genetic study of 34 X-chromosome markers in 5 main ethnic groups of China. Sci Rep 5:17711CrossRefGoogle Scholar