Development and characterization of eleven microsatellite loci for the tropical understory tree Paypayrola blanchetiana Tul. (Violaceae)
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Microsatellites markers were developed for Paypayrola blanchetiana (Violaceae), a near-dispersing forest tree forming aggregated populations, to investigate genetic diversity and gene flow among subpopulations in a fragmented environment. Next generation sequencing (Illumina platform) was used to develop ten nuclear microsatellite loci and one plastid microsatellite locus that amplify in P. blanchetiana. Polymorphism was tested in two subpopulations separated by a distance of approximately 11 km. The identified loci contained between two and five alleles per locus. Observed heterozygosity ranged between 0.063 and 0.563 in both subpopulations, while expected heterozygosity ranged from 0.063 to 0.567 in the first, and 0.063–0.627 in the second subpopulation. The microsatellites are among the first in the family Violaceae and will be useful for population genetic studies in this species. Amplification was successful in one further Paypayrola species from Amazonia, which suggest a wider usefulness of the present markers.
KeywordsCross-amplification Next generation sequencing Polymorphism Primer design SSR
The authors thank Usina São José S.A., municipality of Igarassú (Pernambuco) for facilitating collection of leaf material, and Dr. Theresa Liberal, Bruno Sampaio and the Laboratory of Bioinformatics and Evolutionary Biology (Federal University of Pernambuco, Recife) for providing services of the ABI-3500 sequencing platform. The study was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (Grant No. 300532/2016-4) and Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (Grant No. APQ-0207-2.03/13).
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Conflict of interest
The authors declare that they have no conflict of interest.
The authors hereby declare that (a) the present manuscript has not been submitted to any other journal simultaneously; (b) the present manuscript has not been published previously; (c) the present manuscript covers a completed study (i.e., microsatellite marker development in the plant species Paypayrola blanchetiana); (d) the authors have not fabricated nor manipulated any data presented in the manuscript; (e) no data, text or theories by others are presented by the authors as their own. All references to other authors´ works are duly marked in the text. No humans nor animals were subject to the present study.
All authors consent to the submission of this manuscript to the journal Molecular Biology Reports.
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