Proteomic analysis of the effects of antler extract on chondrocyte proliferation, differentiation and apoptosis
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Deer antlers are unique cranial appendages capable of regeneration and rapid growth. In addition, deer antlers have been widely used in traditional Chinese medicine to promote the function of the kidneys, reproductive system, bones and nervous system. It has been shown that water-soluble substances are the major bioactive components within the deer antlers. In this study, we prepared aqueous extracts from deer antlers during a rapid growth stage. We investigated the effects of antler extracts on primary chondrocytes by analyzing their protein expression patterns using isobaric tags for relative and absolute quantitation technology. We demonstrated that antler extracts promote chondrocyte proliferation and prevent chondrocyte differentiation and apoptosis by controlling multiple cellular processes involved in genomic stability, epigenetic alterations, ribosome biogenesis, protein synthesis and cytoskeletal reorganization. Antler extracts significantly increased the expression levels of proliferation markers Mki67 and Stmn1 and differentiation inhibitor Acp5 as well as cellular apoptosis inhibitors Ndufa4l2 and Rcn1. Thus, this study has greatly expanded our current knowledge of the molecular effects of antler extracts on chondrocytes. It has also shed new light on possible strategies to prevent damage to and to treat cartilage and its related diseases by using aqueous extracts from growing Sika deer antlers.
KeywordsDeer antler extracts Chondrocytes Proteomics Proliferation Differentiation Apoptosis
This work was supported by the National Key Research and Development Program of China (Grant No. 2018YFC1706605), the TCM Clinical Research Center for Bone diseases of Jilin Province (Grant No. 20180623048TC), the Science and Technology Development Project of Jilin Province (Grant No. 20170520044JH), the Science and Technology Project of Jilin Provincial Education Department (Grant No. JJKH20170721KJ), and the National Natural Science Foundation of China (Grant No. 81702136).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
Ethical approval was granted by the ethics committee for the study.
- 6.Chen J, Yang Y, Abbasi S, Hajinezhad D, Kontulainen S, Honaramooz A (2015) Evid Based Complement Altern Med 2015:819520Google Scholar
- 7.Sánchez-Vidaña DI, Rajwani R, Wong MS (2017) The use of omic technologies applied to traditional chinese medicine research. Evid Based Complement Altern Med 2017:6359730Google Scholar
- 8.Ji Q, Zhu F, Liu X, Li Q, Su SB (2015) Recent advance in applications of proteomics technologies on traditional chinese medicine research. Evid Based Complement Altern Med 2015: 983139Google Scholar
- 20.Hamaguchi I, Flygare J, Nishiura H, Brun AC, Ooka A, Kiefer T, Ma Z, Dahl N, Richter J, Karlsson S (2003) Proliferation deficiency of multipotent hematopoietic progenitors in ribosomal protein S19 (RPS19)-deficient diamond-Blackfan anemia improves following RPS19 gene transfer. Mol Ther 7(5):613–622CrossRefGoogle Scholar
- 23.Sobecki M, Mrouj K, Camasses A, Parisis N, Nicolas E, Llères D, Gerbe F, Prieto S, Krasinska L, David A, Eguren M, Birling MC, Urbach S, Hem S, Déjardin J, Malumbres M, Jay P, Dulic V, Lafontaine DLj, Feil R, Fisher D (2016) The cell proliferation antigen Ki-67 organises heterochromatin. Elife 5:e13722CrossRefGoogle Scholar
- 26.Rinaldi L, Sepe M, Delle Donne R, Conte K, Arcella A, Borzacchiello D, Amente S, De Vita F, Porpora M, Garbi C, Oliva MA, Procaccini C, Faicchia D, Matarese G, Zito Marino F, Rocco G, Pignatiello S, Franco R, Insabato L, Majello B, Feliciello A (2017) Mitochondrial AKAP1 supports mTOR pathway and tumor growth. Cell Death Dis 8(6):e2842CrossRefGoogle Scholar
- 29.Vohhodina J, Barros EM, Savage AL, Liberante FG, Manti L, Bankhead P, Cosgrove N, Madden AF, Harkin DP, Savage KI (2017) The RNA processing factors THRAP3 and BCLAF1 promote the DNA damage response through selective mRNA splicing and nuclear export. Nucleic Acids Res 45(22):12816–12833CrossRefGoogle Scholar
- 30.Gu L, Frommel SC, Oakes CC, Simon R, Grupp K, Gerig CY, Bär D, Robinson MD, Baer C, Weiss M, Gu Z, Schapira M, Kuner R, Sültmann H, Provenzano M, Yaspo ML, Brors B, Korbel J, Schlomm T, Sauter G, Eils R, Plass C, Santoro R (2015) BAZ2A (TIP5) is involved in epigenetic alterations in prostate cancer and its overexpression predicts disease recurrence. Nat Genet 47(1):22–30CrossRefGoogle Scholar
- 38.Zhang X, Liu K, Zhang T, Wang Z, Qin X, Jing X, Wu H, Ji X, He Y, Zhao R (2017) Cortactin promotes colorectal cancer cell proliferation by activating the EGFR-MAPK pathway. Oncotarget 8(1):1541–1554Google Scholar
- 42.Pimiento JM, Chen DT, Centeno BA, Davis-Yadley AH, Husain K, Fulp WJ, Wang C, Zhang A, Malafa MP (2015) Annexin A8 Is a prognostic marker and potential therapeutic target for pancreatic cancer. Oncogenesis 6(3):e304Google Scholar